LOCUS LT727280 6625 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMxCAThTNFR55i, complete sequence.
ACCESSION LT727280
VERSION LT727280.1
KEYWORDS .
SOURCE Vector pMxCAThTNFR55i
ORGANISM Vector pMxCAThTNFR55i
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6625)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6625
/organism="Vector pMxCAThTNFR55i"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3905."
/db_xref="taxon:1945357"
regulatory 13..458
/note="Mx"
/regulatory_class="promoter"
CDS 515..>1171
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87977.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
misc_feature 1172..1183
/note="GGSG"
CDS 1184..1864
/transl_table=11
/note="TNFRi"
/protein_id="SJL87978.1"
/translation="MGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSF
SPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALAS
DPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLE
LQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAAL
PPAPSLLR"
misc_feature 1865..2016
/note="3' UTR of TNFRn"
CDS <2071..2145
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87979.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 2146..2212
/note="intron of SV40 small t-antigen"
misc_feature 2213..2764
/note="3' UTR of SV40 small t-antigen"
regulatory 2765..2861
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2765..2861)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2862..3674)
/note="SV40 VP1 with internal deletion"
regulatory complement(3730..3813)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(3910..4310)
/note="pMB1 ori"
CDS complement(4848..5708)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87980.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 6603..6619
/note="SP6"
/regulatory_class="promoter"
BASE COUNT 1689 a 1656 c 1598 g 1682 t
ORIGIN
1 aagcttgggc tgcagtagca gtgctcagac ctctaaacct gtagtcacaa gggacccaga
61 agggctggca gggctcctca gtgattctgg tatggtttgt ctctgaccct tcctagtgca
121 gctggaagaa tccagtgaaa agagaaagtt agtctaggtt gtgttcaact gagaggaggt
181 caggctgaag gctcagcaca gagctggaag ggtcctggga aagcaggcag ccatctgaac
241 tggtgtccat actgcatgtg tcttggtggg ctgagcctac aatatcgatt tcccaacctc
301 agtaccaagc caaggcccct cagggtgagt ttcgtttctg agctcctttc atatgtggtg
361 ggggtgtggg cacagcaagc tcaggctttt tcacggtttc aattctcctc tggaaggggc
421 ggagccagca ctatttaaag aggtctgagc cctggcagga ctctagagga tctcgaggag
481 cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
541 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
601 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
661 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
721 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
781 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
841 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
901 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
961 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
1021 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
1081 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
1141 acagtactgc gatgagtggc agggcggggc gggcggatcc ggcatgggtt taatgtatcg
1201 ctaccaacgg tggaagtcca agctctactc cattgtttgt gggaaatcga cacctgaaaa
1261 agagggggag cttgaaggaa ctactactaa gcccctggcc ccaaacccaa gcttcagtcc
1321 cactccaggc ttcaccccca ccctgggctt cagtcccgtg cccagttcca ccttcacctc
1381 cagctccacc tatacccccg gtgactgtcc caactttgcg gctccccgca gagaggtggc
1441 accaccctat cagggggctg accccatcct tgcgacagcc ctcgcctccg accccatccc
1501 caaccccctt cagaagtggg aggacagcgc ccacaagcca cagagcctag acactgatga
1561 ccccgcgacg ctgtacgccg tggtggagaa cgtgcccccg ttgcgctgga aggaattcgt
1621 gcggcgccta gggctgagcg accacgagat cgatcggctg gagctgcaga acgggcgctg
1681 cctgcgcgag gcgcaataca gcatgctggc gacctggagg cggcgcacgc cgcggcgcga
1741 ggccacgctg gagctgctgg gacgcgtgct ccgcgacatg gacctgctgg gctgcctgga
1801 ggacatcgag gaggcgcttt gcggccccgc cgccctcccg cccgcgccca gtcttctcag
1861 atgaggctgc gcccctgcgg gcagctctaa ggaccgtcct gcgagatcgc cttccaaccc
1921 cacttttttc tggaaaggag gggtcctgca ggggcaagca ggagctagca gccgcctact
1981 tggtgctaac ccctcgatgt acatagcttt tctcaggggg atccccccta ggcttttgca
2041 aaaagctcaa gcttggctct agagtcgacg gatctttgtg aaggaacctt acttctgtgg
2101 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
2161 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
2221 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
2281 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
2341 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
2401 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
2461 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
2521 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
2581 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
2641 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
2701 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
2761 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
2821 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
2881 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccccag gaagctcctc
2941 tgtgtcctca taaaccctaa cctcctctac ttgagaggac attccaatca taggctgccc
3001 atccaccctc tgtgtcctcc tgttaattag gtcacttaac aaaaaggaaa ttgggtaggg
3061 gtttttcaca gaccgctttc taagggtaat tttaaaatat ctgggaagtc ccttccactg
3121 ctgtgttcca gaagtgttgg taaacagccc acaaatgtca acagcagaaa catacaagct
3181 gtcagctttg cacaagggcc caacaccctg ctcatcaaga agcactgtgg ttgctgtgtt
3241 agtaatgtgc aaaacaggag gcacattttc cccacctgtg taggttccaa aatatctagt
3301 gttttcattt ttacttggat caggaaccca gcactccact ggataagcat tatccttatc
3361 caaaacagcc ttgtggtcag tgttcatctg ctgactgtca actgtagcat tttttggggt
3421 tacagtttga gcaggatatt tggtcctgta gtttgctaac acaccctgca gctccaaagg
3481 ttccccacca acagcaaaaa aatgaaaatt tgacccttga atgggttttc cagcaccatt
3541 ttcatgagtt ttttgtgtcc ctgaatgcaa gtttaacata gcagttaccc caataacctc
3601 agttttaaca gtaacagctt cccacatcaa aatatttcca caggttaagt cctcatttaa
3661 attaggcaaa ggaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
3721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
3781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
3841 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
3901 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
3961 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
4021 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
4081 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
4141 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
4201 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
4261 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
4321 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
4381 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
4441 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
4501 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
4561 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
4621 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
4681 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
4741 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
4801 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
4861 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
4921 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
4981 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
5041 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
5101 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
5161 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
5221 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
5281 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
5341 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
5401 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
5461 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
5521 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
5581 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
5641 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
5701 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
5761 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
5821 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
5881 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
5941 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
6001 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
6061 gcatcagagc agattgtact gagagtgcac catatcgacg ctctccctta tgcgactcct
6121 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
6181 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
6241 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
6301 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
6361 cggcgtagag gatctggcta gcgatgaccc tgctgattgg ttcgctgacc atttccgggg
6421 tgcggaacgg cgttaccaga aactcagaag gttcgtccaa ccaaaccgac tctgacggca
6481 gtttacgaga gagatgatag ggtctgcttc agtaagccag atgctacaca attaggcttg
6541 tacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
6601 cgatttaggt gacactatag aatac
//