LOCUS LT727275 7171 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-12a, complete sequence.
ACCESSION LT727275
VERSION LT727275.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-12a
ORGANISM Mammalian expression vector pCAGGS-mCASP-12a
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7171)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7171
/organism="Mammalian expression vector pCAGGS-mCASP-12a"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3856."
/db_xref="taxon:1944878"
misc_feature complement(182..1115)
/note="3UTR"
CDS complement(1116..2375)
/transl_table=11
/note="CASP12"
/protein_id="SJL87965.1"
/translation="MAARRTHERDPIYKIKGLAKDMLDGVFDDLVEKNVLNGDELLKI
GESASFILNKAENLVENFLEKTDMAGKIFAGHIANSQEQLSLQFSNDEDDGPQKICTP
SSPSESKRKVEDDEMEVNAGLAHESHLMLTAPHGLQSSEVQDTLKLCPRDQFCKIKTE
RAKEIYPVMEKEGRTRLALIICNKKFDYLFDRDNADTDILNMQELLENLGYSVVLKEN
LTAQEMETELMQFAGRPEHQSSDSTFLVFMSHGILEGICGVKHRNKKPDVLHDDTIFK
IFNNSNCRSLRNKPKILIMQACRGRYNGTIWVSTNKGIATADTDEERVLSCKWNNSIT
KAHVETDFIAFKSSTPHNISWKVGKTGSLFISKLIDCFKKYCWCYHLEEIFRKVQHSF
EVPGELTQMPTIERVSMTRYFYLFPGN"
misc_feature complement(2376..2408)
/note="5UTR"
regulatory 2461..2552
/note="polyA"
/regulatory_class="terminator"
misc_feature 2553..2909
/note="3FR"
regulatory complement(2987..3069)
/note="lac"
/regulatory_class="promoter"
rep_origin 3211..3286
/note="SV40 ori"
regulatory 3317..3391
/note="polyA"
/regulatory_class="terminator"
regulatory complement(3317..3391)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(3506..3907)
/note="pMB1 ori"
CDS complement(4448..5308)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87966.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5444..5821
/note="hCMV"
/regulatory_class="promoter"
regulatory 5827..7168
/note="AG"
/regulatory_class="promoter"
misc_feature 6196..7120
/note="intron"
BASE COUNT 1697 a 1737 c 1763 g 1974 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gcgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat gtatataact
121 atctattcga tgatgaagat accccaccaa acccaaaaaa agagatcgaa ttcggcacga
181 gttttttttt tttttttttt atagaacaaa gaataaacaa agattttaat ttgagctcag
241 ttaagtaaaa ataatgatat aacatcctca agatgacaaa aatagtggta ggtgggcttc
301 atcacatact tgtagaagaa ttcaattttg catagaattt catagaatat tttgtctcaa
361 aattgtcttt aatgctttaa acttttaatt tttgtttcta ggatttattt caataacact
421 aatagacaga atcttaaatg tgtatgtgtg agtatatctt attacaaagg catttaagag
481 aaaaattata aacatagcat aaatttgtca attatatcct aagaaattga ttgaaaggaa
541 tggtaggtag ccactaggca tgaaaaccat gtatgccaga gacaaacagt ggacataaac
601 ctctttatct tattcctaaa agatgattag ttttacctac atatgcaaca gtttcatttc
661 tcagctacat tgtagataag atcttccttg tgagtaaatt cttactacta atttataggt
721 gaaagtggtt catcaaaagg tgtatcatct ttgttcttta gtgtcttttt cctttattca
781 ttttacaaag aagaagttcc aggatatgtt cagacatgtt ctttagtgtc tttttccttt
841 attcatttta caaaaatgtg caaatataaa tttaataaaa taaagtaaaa gatgagcaga
901 tttggaagga aggaacgaat ctatactttt catgcaaaat tttacgtatc aggtttcccc
961 taaaaataac ctatggaaat tatctagcct tatgatattg tataaaatat ctaactgaaa
1021 agttttacac atttaaaaat cctgggatct tggaattttc ttttcttctc agctacagca
1081 atacttgact gggaactgca tgagagttgc ctgtgctaat tcccgggaaa aaggtagaaa
1141 tagcgtgtca tggatactct ctcaatagtg ggcatctggg tcagttcacc tgggacctca
1201 aatgagtgtt gaacctttcg aaaaatttcc tccaaatgat aacaccaaca gtactttttg
1261 aagcagtcaa tgagtttgga aatgaagagg gaaccagtct tgcctacctt ccaagaaata
1321 ttatgtgggg tagaagattt gaaagcaatg aaatctgtct ccacatgggc ctttgttata
1381 ctattattcc atttacagct caacacacgt tcctcatctg tatcagcagt ggctatccct
1441 ttgtttgtgg atacccaaat agttccatta tatctgcctc tgcaggcctg catgatgaga
1501 atcttgggtt tgtttctcag actccgacag ttagagttgt tgaaaatttt gaagatagtg
1561 tcatcatgaa gaacatctgg ctttttgttt cggtgcttca ccccacagat tccttccagg
1621 atgccatggg acataaacac caggaatgtg ctgtctgagg actggtgctc tggacggcca
1681 gcaaactgca ttaactctgt ctccatttcc tgagctgtaa ggttttcctt taacaccaca
1741 gagtatccaa gattttcaag tagttcttgc atgttcaaaa tgtcagtatc agcattatct
1801 ctatcaaaaa ggtagtcaaa ctttttgttg cagatgatga gagccagacg tgttcgtccc
1861 tccttctcca tcactggata tatctctttt gccctttctg tctttatctt acaaaactga
1921 tcacgtggac aaagcttcag tgtatcttgg acttctgagc tctggagtcc atgaggagct
1981 gtcagcatta gatgtgattc atgggccaat ccagcattta cctccatttc atcatcctct
2041 acttttctct tggattctga tggagaagaa ggtgtacata tcttctgagg tccatcatcc
2101 tcatcattag aaaattgtaa actcagctgt tcctgggaat tggcaatgtg gccagcaaat
2161 atttttcctg ccatgtctgt tttctctaag aagttctcaa ccagattctc agccttgttc
2221 aggatgaaac tcgcactttc ccctattttg agtaactcat ctccatttaa aacattcttc
2281 tccaccaggt catcaaaaac cccatccagc atgtccttgg ccaaaccttt gatcttgtag
2341 attggatctc tttcatgtgt cctcctggcc gccatggctg tgctcgtgcc gctcgtgccg
2401 ctcgtgccct cgtgccgaat tcccggggat cccaatgccc tggctcacaa ataccactga
2461 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
2521 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
2581 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
2641 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
2701 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
2761 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
2821 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
2881 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
2941 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
3001 agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact cacattaatt
3061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
3121 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
3181 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
3241 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
3301 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
3361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
3421 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
3481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
3541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
3601 acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
3661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
3721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
3781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
3841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
3901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
3961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
4021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
4081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
4141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
4201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
4261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
4321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
4381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
4441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
4501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
4561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
4621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
4681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
4741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
4801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
4861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
4921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
4981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
5041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
5101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
5161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
5221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
5281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
5341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
5401 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact
5461 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
5521 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
5581 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
5641 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
5701 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
5761 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
5821 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
5881 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
5941 ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa
6001 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
6061 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc
6121 cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
6181 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
6241 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
6301 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
6361 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
6421 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
6481 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
6541 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
6601 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
6661 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
6721 gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
6781 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
6841 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
6901 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
6961 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
7021 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
7081 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
7141 tattgtgctg tctcatcatt ttggcaaaga a
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