LOCUS LT727274 5633 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGSmIL1B, complete sequence.
ACCESSION LT727274
VERSION LT727274.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGSmIL1B
ORGANISM Mammalian expression vector pCAGGSmIL1B
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5633)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5633
/organism="Mammalian expression vector pCAGGSmIL1B"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3853."
/db_xref="taxon:1944889"
CDS 76..885
/transl_table=11
/note="mIL1B"
/protein_id="SJL87963.1"
/translation="MATVPELNCEMPPFDSDENDLFFEVDGPQKMKGCFQTFDLGCPD
ESIQLQISQQHINKSFRQAVSLIVAVEKLWQLPVSFPWTFQDEDMSTFFSFIFEEEPI
LCDSWDDDDNLLVCDVPIRQLHYRLRDEQLKSLVLSDPYELKALHLNGQNINQQVIFS
MSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVF
NKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS"
regulatory 923..1014
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1015..1371
/note="3' FR of beta-globin"
regulatory complement(1449..1531)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1673..1748
/note="SV40 ori"
regulatory 1779..1853
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1779..1853)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1968..2369)
/note="pMB1 ori"
CDS complement(2910..3770)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87964.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3906..4283
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4289..5630
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4658..5582
/note="intron AG promoter"
BASE COUNT 1243 a 1481 c 1529 g 1380 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gatcagcgct ccaccatggc aactgttcct gaactcaact gtgaaatgcc accttttgac
121 agtgatgaga atgacctgtt ctttgaagtt gacggacccc aaaagatgaa gggctgcttc
181 caaacctttg acctgggctg tcctgatgag agcatccagc ttcaaatctc gcagcagcac
241 atcaacaaga gcttcaggca ggcagtatca ctcattgtgg ctgtggagaa gctgtggcag
301 ctacctgtgt ctttcccgtg gaccttccag gatgaggaca tgagcacctt cttttccttc
361 atctttgaag aagagcccat cctctgtgac tcatgggatg atgatgataa cctgctggtg
421 tgtgacgttc ccattagaca actgcactac aggctccgag atgaacaact aaaaagcctc
481 gtgctgtcgg acccatatga gctgaaagct ctccacctca atggacagaa tatcaaccaa
541 caagtgatat tctccatgag ctttgtacaa ggagaaccaa gcaacgacaa aatacctgtg
601 gccttgggcc tcaaaggaaa gaatctatac ctgtcctgtg taatgaaaga cggcacaccc
661 accctgcagc tggagagtgt ggatcccaag caatacccaa agaagaagat ggaaaaacgg
721 tttgtcttca acaagataga agtcaagagc aaagtggagt ttgagtctgc agagttcccc
781 aactggtaca tcagcacctc acaagcagag cacaagcctg tcttcctggg aaacaacagt
841 ggtcaggaca taattgactt caccatggaa tctgtgtctt cctaagcgct gatccaatgc
901 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
961 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
1021 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
1081 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
1141 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
1201 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
1261 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
1321 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
1381 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
1441 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg
1501 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
1561 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
1621 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
1681 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
1741 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
1801 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
1861 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
1921 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
1981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
2041 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca
2101 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
2161 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
2221 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2281 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
2341 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
2401 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
2461 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
2521 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
2581 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
2641 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
2701 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
2761 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
2821 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
2881 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
2941 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
3001 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
3061 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
3121 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
3181 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
3241 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
3301 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
3361 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
3421 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
3481 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
3541 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
3601 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
3661 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
3721 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
3781 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
3841 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
3901 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
3961 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
4021 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
4081 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
4141 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
4201 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
4261 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
4321 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
4381 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
4441 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
4501 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
4561 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
4621 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
4681 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
4741 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
4801 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
4861 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
4921 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
4981 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
5041 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
5101 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
5161 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
5221 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
5281 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
5341 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
5401 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
5461 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
5521 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
5581 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa
//