LOCUS LT727272 10145 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pGAD-mCASP-2, complete sequence.
ACCESSION LT727272
VERSION LT727272.1
KEYWORDS .
SOURCE Yeast expression vector pGAD-mCASP-2
ORGANISM Yeast expression vector pGAD-mCASP-2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 10145)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..10145
/organism="Yeast expression vector pGAD-mCASP-2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3850."
/db_xref="taxon:1945477"
regulatory 8..411
/note="ADH1"
/regulatory_class="promoter"
CDS 422..>829
/transl_table=11
/note="GALad"
/protein_id="SJL87957.1"
/translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE"
misc_feature complement(847..2865)
/note="3UTR"
CDS complement(2866..4224)
/transl_table=11
/note="mCASP2"
/protein_id="SJL87958.1"
/translation="MAAPSGRSQSSLHRKGLMAADRRSRILAVCGMHPDHQETLKKNR
VVLAKQLLLSELLEHLLEKDIITLEMRELIQAKGGSFSQNVELLNLLPKRGPQAFDAF
CEALRETRQGHLEDLLLTTLSDIQHVLPPLSCDYDTSLPFSVCESCPPHKQLRLSTDA
TEHSLDNGDGPPCLLVKPCTPEFYQAHYQLAYRLQSQPRGLALVLSNVHFTGEKDLEF
RSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLS
HGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDG
KNHTQSPGCEESDAGKEELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQ
VFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLFPGYP
PT"
misc_feature complement(4225..4309)
/note="5UTR"
regulatory 4727..4828
/note="ADH1T"
/regulatory_class="terminator"
CDS complement(5032..6126)
/transl_table=11
/note="LEU2"
/protein_id="SJL87959.1"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
AGIRTGDLGGSNSTTEVGDAVAEEVKKILA"
rep_origin complement(6791..7193)
/note="pMB1 ori"
CDS complement(7734..8594)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87960.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 9165..10145
/note="2mu ori"
BASE COUNT 2958 a 2324 c 2196 g 2667 t
ORIGIN
1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
841 cacgagatca acatttattt ggcacctgat ggcaatacaa aatcttggca gtgggaagtg
901 tgaagattct accacttatg catcctatac tgacccaaaa ggcagaattt ggcagcaagt
961 cttcaaacca cttggttcat acaacaaaca ttagttgagc acctcaacta ccataggcct
1021 caaatttgct gggcactatg gagatgtgaa agaagttgtc tcacacgtga tatgtagaaa
1081 atataaaaaa acaacaacaa aacagtggat aaaaataaaa cacctggaaa aaaaaaccca
1141 tatactaatt gaactgtaca ctataatagc aatttagaaa ctgcaaaagg taggctctaa
1201 ctaaggttag ctgctggtct ccttccttta ctcaggaaac attcatgaag acctagtaag
1261 tgccagttac tggatatcag taagtaagaa gcacagttcc cactctcttg gagcattcag
1321 cctcagaggg aacaagtgaa acaagcaaaa gtgccattac agcctgcagt aagcacaaca
1381 gagaccaaca tgatgccctc ataaggaggc acagtagaca gcatgggcag catggtcaag
1441 aggcctccgc cagactcaaa aatcagagac tagccaaagg agagttcaga acacctgcaa
1501 gacggagatg aggacctaag aggggctgga gcatagtgag aaatcaccat ttaatgagat
1561 ccaaagggaa ggtatgagcc agatctcgta agctgggtgt aagaatttgc attttagatg
1621 aagcgtaaca ggaagctagt gttctaagca agagaatgac atcaccagat attttaaaaa
1681 ccaaactact ctgttacatg gagaataaat tggaagagag gaaaaaatgg aaatgcagaa
1741 ggttttcaag aataagcaag tttcataaga gcagagacaa gtgcttctag gttatatcac
1801 atatggtact atccccacta aaacacagag aaacaatgag aagggagacg ctgccacaga
1861 aaaccaaaga agcctcacgc tgaagcagaa ggcagaaaga ataagcaact acagtacagc
1921 gtaagtggca agaaacatcc tacaaaggac ggcttcacag acttggcaga agcagcgaat
1981 ggtgaaggta aagaggggaa ccagtacaaa gcaagagaga ccctggaggc cagccgtcaa
2041 gtacagtgta agtgcatgca gaagccacag taaggaagga gagacatcct gatgacgggt
2101 gatagtgtga gacacagcac actcagctgt cactgcattt tcatgtagtt atgcccgata
2161 aggttcattc agtgacagtt caaacaaagc atgaccaggg aaatttctat gacttccagt
2221 gtcatggcat acagacccta ggctctgttc aaaggctggg tgcactgaac acaaaggaac
2281 caatggaaag gctgagggac tgtccttcac ctagggcctg aacttaaagg tatgcataga
2341 aacttcgcaa gtagtaggat ctcatctgtc ccttcatgac agatgaaaac acctacatta
2401 aattgggaca tcttccaaat ctccactgtg ggaaaaatga ggttgtagat atggaaaaaa
2461 aaggagatga tctctgagac tccttaactg atttaggaaa aaaaaaaaaa gctatgatcc
2521 aagtactcct aagatgcctt gggaacaaaa atatcacaaa cattgagaaa tccaaatgcc
2581 ctcttcccac aataatactg tgttcacaat gtcattttct aggctgtagc taaaatgctt
2641 ctggtaacac atccattcag agctggctct ataggtagag aacacgacac cggaggagcc
2701 atccttatgt ttcataccca aagacagaga tggctgacag gaaacaagcc tgggagaatc
2761 tcatatccct gagagggtag acagaacaaa aaccattctg aagagaagtc tccattgtgc
2821 ccccagtggt ccagtggcct ccaacagcag gaatagcagg cggcatcacg tgggtgggta
2881 gcctgggaac aggtagagtt gctggcacag agtactacag tactcagaca tctccttgca
2941 tcggtggaat tctgtgccag gggcatagcc ttcacgctcc ttgataaggg cattcacctt
3001 aacaagcatg tcggccacgt gcatgtcaca agctctttca gagaacacct gagtgagggc
3061 ctcaatgtac caggaacccc gtttggtgtt ccgcatggca gcattacctt taaggcaagc
3121 atagccacat atcatgtctg agcgagtagg cagtctcatc ttcatcaact cctctttgcc
3181 agcatcactc tcctcacatc caggggattg tgtgtggttc tttccatctt gctggtcgac
3241 acctctatct gtctcatctc cacgacatgc ttggatgaag aacatttttg gcttgttctg
3301 tagacttgga cagttagcat tgtcaaaaag tcgaaaaacc tcttggagct gaagcagttt
3361 gccatctaca ccatagatgc caccttccac accatgtgag aggagtgcca ctacgcagga
3421 gtctgtgacc cggtgtgcag gtaactgtgc aaaattctga agtttctctt gcatttcctg
3481 tgcggtctgg tcatgtagca catggacatt gtagcccaaa agcttgaaga gggtgactag
3541 agtagtgtgg tccacatccc ctccagagcg gaattccagg tctttctctc cagtgaagtg
3601 cacattgctc agcaccagtg ccaagccacg gggctgagat tgcaacctat aggccagctg
3661 gtagtgtgcc tggtaaaact caggagtgca tggcttcacc agaagacagg gaggaccatc
3721 accattatct aaggagtgtt ccgtagcatc tgtggatagg cggagctgct tgtgaggagg
3781 gcaggactca cacaccgaga aagggagact tgtgtcatag tcacagctca acggtgggag
3841 tacgtgctga atatctgaga gggttgtgag cagtaagtct tccaagtgac cctgcctggt
3901 ctcccgcagg gcttcacaga aggcatcaaa agcctggggt cctctctttg gcagcaggtt
3961 gaggagttcc acattctggc tgaaactgcc ccctttggcc tggatgagct ccctcatttc
4021 caaagtgata atgtccttct ctaggaggtg ttctaacagc tcgctcagca gcagctgctt
4081 ggccagcacc actcgattct ttttcagtgt ttcctggtgg tcaggatgca ttccacacac
4141 tgccaaaatc ctgctcctcc tgtcagccgc catcagcccc ttcctgtgca gggaggactg
4201 cgacctcccg ctcggcgccg ccatttccag cttttcccgg gctgcacacg caccacccac
4261 aaaacaaaca aacgctgggc cgcgccagcc cccgcatccc tcccccagcc ctcgtgccga
4321 attcccgggg atccgtcgac ctgcagagat ctatgaatcg tagatactga aaaaccccgc
4381 aagttcactt caactgtgca tcgtgcacca tctcaatttc tttcatttat acatcgtttt
4441 gccttctttt atgtaactat actcctctaa gtttcaatct tggccatgta acctctgatc
4501 tatagaattt tttaaatgac tagaattaat gcccatcttt tttttggacc taaattcttc
4561 atgaaaatat attacgaggg cttattcaga agctttggac ttcttcgcca gaggtttggt
4621 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
4681 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
4741 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
4801 tgactcttag gttttaaaac gaaaattctt gttcttgagt aactctttcc tgtaggtcag
4861 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
4921 cgaaattccc ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg
4981 aatttcattt ataaagttta tgtacaaata tcataaaaaa agagaatctt tttaagcaag
5041 gattttctta acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc
5101 taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt
5161 accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc
5221 gatagggtca accttattct ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa
5281 accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa
5341 ggaacctggg ataacggagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat
5401 tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa
5461 ttgatgttga accttcaatg taggaaattc gttcttgatg gtttcctcca cagtttttct
5521 ccataatctt gaagaggcca aaacattagc tttatccaag gaccaaatag gcaatggtgg
5581 ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt
5641 gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta
5701 aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt
5761 gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta
5821 caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac tacctgtacc
5881 ccatttagga ccacccacag cacctaacaa aacggcatca accttcttgg aggcttccag
5941 cgcctcatct ggaagtggga cacctgtagc atcgatagca gcaccaccaa ttaaatgatt
6001 ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc
6061 ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggggc
6121 agacattaga atggtatatc cttgaaatat atatatatat tgctgaaatg taaaaggtaa
6181 gaaaagttag aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa
6241 ataatattgt caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt
6301 ctatatgaaa agccggttcc ggcctctcac ctttcctttt tctcccaatt tttcagttga
6361 aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa aatttccagt catcgaattt
6421 gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg
6481 ctaagagatt cgaactcttg catcttacga tacctgagta ttcccacagt tggggatctc
6541 gactctagct agaggatcaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
6601 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
6661 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
6721 ggaaacctgt cgtgccagct ggattaatga atcggccaac gcgcggggag aggcggtttg
6781 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
6841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
6901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
6961 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
7021 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
7081 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
7141 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
7201 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
7261 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
7321 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
7381 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
7441 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
7501 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
7561 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
7621 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
7681 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
7741 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
7801 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
7861 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
7921 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
7981 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
8041 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
8101 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
8161 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
8221 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
8281 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
8341 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
8401 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
8461 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
8521 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
8581 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
8641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
8701 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
8761 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
8821 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
8881 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
8941 tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa gcataaacac
9001 gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat ataggtgcga
9061 cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg
9121 gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag tataggaact
9181 tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg
9241 caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa
9301 aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc catccacctt
9361 tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat
9421 tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac
9481 gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata
9541 attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa
9601 tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc
9661 ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat
9721 agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga
9781 gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa
9841 atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta
9901 aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg
9961 aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt
10021 tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt
10081 gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct
10141 tcgtt
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