LOCUS LT727271 7910 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pGAD-mCASP-6, complete sequence.
ACCESSION LT727271
VERSION LT727271.1
KEYWORDS .
SOURCE Yeast expression vector pGAD-mCASP-6
ORGANISM Yeast expression vector pGAD-mCASP-6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7910)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7910
/organism="Yeast expression vector pGAD-mCASP-6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3849."
/db_xref="taxon:1945479"
regulatory 8..411
/note="ADH1"
/regulatory_class="promoter"
CDS 422..>829
/transl_table=11
/note="GALad"
/protein_id="SJL87953.1"
/translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE"
misc_feature complement(847..1220)
/note="3UTR"
CDS complement(1221..2051)
/transl_table=11
/note="mCASP6"
/protein_id="SJL87954.1"
/translation="MTETDGFYKSREVFDPAEQYKMDHKRRGVALIFNHERFFWHLTL
PERRGTNADRDNLTRRFSDLGFEVKCFNDLRAEELLLKIHEVSTSSHIDADCFICVFL
SHGEGNHVYAYDAKIEIQTLTGLFKGDKCQSLVGKPKIFIIQACRGSQHDVPVVPLDM
VDHQTDKLDNVTQVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEML
ARYGSSLEFTELLTLVNRKVSQRRVDFCKDPDAIGKKQVPCFASMLTKKLHFCPKPSK
"
misc_feature complement(2052..2075)
/note="5UTR"
regulatory 2492..2593
/note="ADH1T"
/regulatory_class="terminator"
CDS complement(2797..3891)
/transl_table=11
/note="LEU2"
/protein_id="SJL87955.1"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
AGIRTGDLGGSNSTTEVGDAVAEEVKKILA"
rep_origin complement(4556..4958)
/note="pMB1 ori"
CDS complement(5499..6359)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87956.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 6930..7910
/note="2mu ori"
BASE COUNT 2222 a 1796 c 1658 g 2234 t
ORIGIN
1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
841 cacgaggttc ttagacattt tattacgcga aaacaatctc agtctagagt ttcaaaagtc
901 tttacaaaca ttatacagta aactcatata aacagtgtca caaaagggga ggcaaagtag
961 ttggtattta gagtaatctt acagtttact aaaaatgtaa taaatgtagt agacattgat
1021 gcatagtaat ttcctgagga ttatttttag cccttccacc acgtccaaca tgattgaaaa
1081 gtaacaaata taaaacatta cttttctatg gcatctacaa aattgaagca gtttacattg
1141 atgggtgaaa gtcttccacc agctggcctt tgtataaaaa gggaaatgtg tgtgaaatat
1201 atgtagcaag acagatggcc ctacttgcta ggtttgggac agaaatgcag ctttttggtc
1261 agcattgagg cgaagcaggg cacctgcttc ttgccgattg catccgggtc tttgcagaag
1321 tccacgcggc gctgagagac ctttctgttc accagcgtga gcagctccgt gaactccagg
1381 gaactgccgt acctcgccag catctcacac aaatcctgaa tgtaccagga gccattcaca
1441 gtttctcggt gagagtaata cccttctgcg acagagtagc acatgaggaa gtctgcccct
1501 gccggcagcg tgtacacgga cgcagcatcc acctgggtca cgttgtccag cttgtctgtc
1561 tggtgatcca ccatgtccag gggaaccacg ggtacgtcat gctggctacc ccgacaggcc
1621 tggatgataa atatcttggg ttttccaacc aggctctgac acttgtctcc tttgaacaag
1681 ccagtcaacg tctgaatttc aattttggcg tcgtatgcgt aaacgtggtt gccttcccca
1741 tggctcagga agacacagat gaagcaatcg gcatctatgt ggcttgaagt cgacacctcg
1801 tgaattttga gcaggagttc ttctgctctg aggtcgttaa agcatttcac ttcaaatcct
1861 agatctgaaa acctgcgagt caggttgtct ctgtctgcgt tggtgccccg cctctctggg
1921 agggtcaggt gccaaaagaa cctctcgtga ttgaagatga gggcaactcc tctcctcttg
1981 tggtccatct tgtactgctc ggctggatcg aacacttccc tacttttgta gaagccatcg
2041 gtttctgtca tgttttactt tcacctgccc gcgagctcgt gccgaattcc cggggatccg
2101 tcgacctgca gagatctatg aatcgtagat actgaaaaac cccgcaagtt cacttcaact
2161 gtgcatcgtg caccatctca atttctttca tttatacatc gttttgcctt cttttatgta
2221 actatactcc tctaagtttc aatcttggcc atgtaacctc tgatctatag aattttttaa
2281 atgactagaa ttaatgccca tctttttttt ggacctaaat tcttcatgaa aatatattac
2341 gagggcttat tcagaagctt tggacttctt cgccagaggt ttggtcaagt ctccaatcaa
2401 ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt
2461 tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta tgatttttat
2521 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
2581 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
2641 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcccgaaa ttcccctacc
2701 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
2761 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
2821 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
2881 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
2941 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggtcaacctt
3001 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
3061 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
3121 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
3181 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
3241 caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
3301 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
3361 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
3421 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
3481 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
3541 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
3601 acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt taggaccacc
3661 cacagcacct aacaaaacgg catcaacctt cttggaggct tccagcgcct catctggaag
3721 tgggacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt
3781 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc
3841 ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttagaatggt
3901 atatccttga aatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt
3961 aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat attgtcaact
4021 tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata tgaaaagccg
4081 gttccggcct ctcacctttc ctttttctcc caatttttca gttgaaaaag gtatatgcgt
4141 caggcgacct ctgaaattaa caaaaaattt ccagtcatcg aatttgattc tgtgcgatag
4201 cgcccctgtg tgttctcgtt atgttgagga aaaaaataat ggttgctaag agattcgaac
4261 tcttgcatct tacgatacct gagtattccc acagttgggg atctcgactc tagctagagg
4321 atcaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
4381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg
4441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
4501 cagctggatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
4561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
4621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4921 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
5041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
5101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
5161 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
5221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
5281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
5341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
5401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
5521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
5581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
5641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
5821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
6001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
6061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
6121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
6181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
6241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
6301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
6361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
6421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
6481 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
6541 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
6601 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
6661 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
6721 cagattgtac tgagagtgca ccataacgca tttaagcata aacacgcact atgccgttct
6781 tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct
6841 gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt
6901 tcctattccg aagttcctat tctctagcta gaaagtatag gaacttcaga gcgcttttga
6961 aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg
7021 agctactaaa atattgcgaa taccgcttcc acaaacattg ctcaaaagta tctctttgct
7081 atatatctct gtgctatatc cctatataac ctacccatcc acctttcgct ccttgaactt
7141 gcatctaaac tcgacctcta cattttttat gtttatctct agtattactc tttagacaaa
7201 aaaattgtag taagaactat tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt
7261 tcctatacgt agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg
7321 aagaatcatc aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag
7381 aatataatcg gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag
7441 taaacgcggg aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc
7501 cttcttctaa ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag
7561 cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga
7621 tgcatttttg tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc
7681 gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct
7741 cgcgttgcat ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct
7801 ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
7861 gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt
//