LOCUS LT727268 9053 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pGAD-mCASP-7, complete sequence. ACCESSION LT727268 VERSION LT727268.1 KEYWORDS . SOURCE Yeast expression vector pGAD-mCASP-7 ORGANISM Yeast expression vector pGAD-mCASP-7 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9053) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..9053 /organism="Yeast expression vector pGAD-mCASP-7" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3846." /db_xref="taxon:1945480" regulatory 8..411 /note="ADH1" /regulatory_class="promoter" CDS 422..>829 /transl_table=11 /note="GALad" /protein_id="SJL87941.1" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE" misc_feature complement(847..2117) /note="3' UTR of mCASP7" CDS complement(2118..3029) /transl_table=11 /note="mCASP7" /protein_id="SJL87942.1" /translation="MTDDQDCAAELEKVDSSSEDGVDAKPDRSSIISSILLKKKRNAS AGPVRTGRDRVPTYLYRMDFQKMGKCIIINNKNFDKATGMDVRNGTDKDAGALFKCFQ NLGFEVTVHNDCSCAKMQDLLRKASEEDHSNSACFACVLLSHGEEDLIYGKDGVTPIK DLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDIDANPRNKIPVEAD FLFAYSTVPGYYSWRNPGKGSWFVQALCSILNEHGKDLEIMQILTRVNDRVARHFESQ SDDPRFNEKKQIPCMVSMLTKELYFSR" misc_feature complement(3030..3218) /note="5' UTR of mCASP7" regulatory 3635..3736 /note="ADH1T" /regulatory_class="terminator" CDS complement(3940..5034) /transl_table=11 /note="LEU2" /protein_id="SJL87943.1" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA" rep_origin complement(5699..6101) /note="pMB1 ori" CDS complement(6642..7502) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87944.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 8073..9053 /note="2mu ori" BASE COUNT 2508 a 2080 c 1962 g 2503 t ORIGIN 1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga 121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac 181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct 241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga 301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa 421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg 481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt 541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc 601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag 661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc 721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata 781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg 841 cacgagtttt tttttttttt tttttttttt ttttctgaaa cacattttaa tcaataaata 901 ggaacatctg tcaaccattt attaggtggg aaaaacagga atgtataacg aattccaaag 961 aaaatgatac cttggaaaga aactgctaag atcttcgcct aaattgtcat ttgtagaacc 1021 agaggtaacc aaagattatt cagaaaaaaa aatcttggaa tatttgaaag aattccagat 1081 gtatttgaag tacaaaaaca ttatcttgta tacaaagtga aatgggttac tcttcagcac 1141 ctgtgtggat ggatgatctg acaaatcagt gaatagtgaa tgaatacatt gtagcacgcc 1201 agaggacatg gttatttaat tcacattttg agccaagttt acccccacca ctacccctgc 1261 tttgctatcc cttcctctgc tctgtagcac agggtagcat ggtccctagg ccctcactag 1321 ttggctttca ggcaccttgg aattataagg cgctggtgga tatgggcaca ggcctgggga 1381 ggcctttggt gtctgactca gttctggctt ggtttccatc tcaggcagtg gcttgtcctg 1441 agcctggctc caatcaccat agtcctccat ggttctagtc tctagaaggc tgccctgcca 1501 cagttcttac tgatgacaag ggaaccgaag tcctcttaca gccccacaga ggcatgtgag 1561 gtaacgggat tgactgtctc tcaattaccc gagtaagtga attccctgta ttgacccttc 1621 tgcaccgata ttcctaggat ggcttatttt ctggattggg ttctatatac tggaatatta 1681 actgtcgtct tctctcagcc acctgggaag gtcggcttaa aaatctgccc taagccactg 1741 agaacaacca ctcagcagcc acctttgcca atggaggcca acaggtgtcg cagctgtatt 1801 ctttcatcaa gagctgggat tctctttgcc aagttcaact gagacgtcat tgccaatgtc 1861 ttctccagag gtcatgctca cagcatgcac tcagcgagac gacaaagaaa aaaagaaaca 1921 gaaacaggaa atcaacctta gaggaagagc atgaagtcat cagatgaaca aagttattaa 1981 acaaaaaaaa aaaaatctag actttcctga aattaaatcc cctcaacatt tctggatatt 2041 ttgagagcat cggaacaaaa ataaaataaa aactggattc atcaacgaac ggcggcaggt 2101 tctcagctga agggtggtca acggctgaag tacagctctt tggtgagcat ggacaccata 2161 cacgggatct gcttcttctc gttgaagcgt ggatcatcag actgggactc gaagtgcctg 2221 gccaccctgt cgttcaccct ggtcaggatc tgcatgatct cgaggtcctt gccatgctca 2281 ttcaggatgg agcagagggc ctgcacaaac caggagcctt tccctgggtt cctccatgag 2341 taataacctg gaaccgtgga gtaagcaaag aggaagtcgg cttccaccgg gatcttgttg 2401 cggggattag cgtcaatgtc gttgatgggc cccgagtcag cctggattcc atcatcgagc 2461 tccgtccctc ggcatgcctg aatgaagaag agtttgggtt tctctaacag ggttttgcat 2521 cggtctcccc taaaatgagc tgtcagatcc tttatgggtg tcacgccatc tttcccgtaa 2581 atcaggtcct cttccccgtg gctcagcagg acgcaggcga agcaggccga gttgctgtgg 2641 tcctcctcag aggcttttct aagcagatct tgcatctttg cacaagagca gtcattgtgg 2701 acggttactt caaaacccag gttttggaag cacttgaaga gggcccctgc atctttgtcc 2761 gtcccattcc ggacgtccat acctgtcgct ttgtcgaagt tcttgttgtt tatgatgatg 2821 catttaccca tcttctggaa atccatgcgg tacagataag tgggcactcg gtcccggccg 2881 gtcctgacgg ggcccgcaga ggcatttctc ttcttcttca agagaataga ggagatgata 2941 gaggagcggt ctggcttggc gtcaactccg tcttcgctgg aagaatccac cttttccagc 3001 tccgcagcac agtcctgatc atcggtcatc gttcccacgg cggcggccag gagcccacgg 3061 cagcgtccgt cctccgcaag ggtcgagggc agctttggga aaactgaaag caaaagtccc 3121 ggaggcggag ggggaggagc gggcgggccg gacggggctg tgcctgcagc ccgggaaagc 3181 tgggggcgag cgggaacgcg cgctacggag cccgccgcct cgtgccgaat tcccggggat 3241 ccgtcgacct gcagagatct atgaatcgta gatactgaaa aaccccgcaa gttcacttca 3301 actgtgcatc gtgcaccatc tcaatttctt tcatttatac atcgttttgc cttcttttat 3361 gtaactatac tcctctaagt ttcaatcttg gccatgtaac ctctgatcta tagaattttt 3421 taaatgacta gaattaatgc ccatcttttt tttggaccta aattcttcat gaaaatatat 3481 tacgagggct tattcagaag ctttggactt cttcgccaga ggtttggtca agtctccaat 3541 caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat 3601 cgttggtaga tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt 3661 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt 3721 tttaaaacga aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca 3781 ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcatgcccg aaattcccct 3841 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat 3901 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac 3961 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt 4021 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg 4081 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggtcaac 4141 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt 4201 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat 4261 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt 4321 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac 4381 cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga 4441 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag 4501 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact 4561 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac 4621 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa 4681 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc 4741 tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc 4801 acccacagca cctaacaaaa cggcatcaac cttcttggag gcttccagcg cctcatctgg 4861 aagtgggaca cctgtagcat cgatagcagc accaccaatt aaatgatttt cgaaatcgaa 4921 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat 4981 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat 5041 ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa 5101 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca 5161 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag 5221 ccggttccgg cctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg 5281 cgtcaggcga cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga 5341 tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg 5401 aactcttgca tcttacgata cctgagtatt cccacagttg gggatctcga ctctagctag 5461 aggatcaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 5521 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 5581 aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 5641 tgccagctgg attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5701 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 6001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 6061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 6121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 6181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 6241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 6301 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 6361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 6481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 6541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 6601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 6661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 6721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 6781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 6841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 6901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 6961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 7021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 7081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 7141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 7201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 7261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 7321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 7381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 7441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 7501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 7561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 7621 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 7681 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 7741 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 7801 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca 7861 gagcagattg tactgagagt gcaccataac gcatttaagc ataaacacgc actatgccgt 7921 tcttctcatg tatatatata tacaggcaac acgcagatat aggtgcgacg tgaacagtga 7981 gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct cgttttcgga aacgctttga 8041 agttcctatt ccgaagttcc tattctctag ctagaaagta taggaacttc agagcgcttt 8101 tgaaaaccaa aagcgctctg aagacgcact ttcaaaaaac caaaaacgca ccggactgta 8161 acgagctact aaaatattgc gaataccgct tccacaaaca ttgctcaaaa gtatctcttt 8221 gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc gctccttgaa 8281 cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta ctctttagac 8341 aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga aaatgtaaac 8401 atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat tttctgacca 8461 atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc cacatcggta 8521 tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt cgctagtaat 8581 cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag acaccaaagt 8641 agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga ctgcattata 8701 gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat agcgctctcg 8761 ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa atagcgctct 8821 cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc 8881 tctcgcgttg catttttgtt ttacaaaaat gaagcacaga ttcttcgttg gtaaaatagc 8941 gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt 9001 agcgctctcg cgttgcattt ttgttctaca aaatgaagca cagatgcttc gtt //