LOCUS LT727267 8026 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pGAD-mCASP-11, complete sequence. ACCESSION LT727267 VERSION LT727267.1 KEYWORDS . SOURCE Yeast expression vector pGAD-mCASP-11 ORGANISM Yeast expression vector pGAD-mCASP-11 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8026) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..8026 /organism="Yeast expression vector pGAD-mCASP-11" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3845." /db_xref="taxon:1945475" regulatory 8..411 /note="ADH1" /regulatory_class="promoter" CDS 422..>829 /transl_table=11 /note="GALad" /protein_id="SJL87937.1" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE" misc_feature complement(847..1041) /note="3' UTR of mCASP11" CDS complement(1042..2163) /transl_table=11 /note="mCASP11" /protein_id="SJL87938.1" /translation="MAENKHPDKPLKVLEQLGKEVLTEYLEKLVQSNVLKLKEEDKQK FNNAERSDKRWVFVDAMKKKHSKVGEMLLQTFFSVDPGSHHGEANLEMEEPEESLNTL KLCSPEEFTRLCREKTQEIYPIKEANGRTRKALIICNTEFKHLSLRYGANFDIIGMKG LLEDLGYDVVVKEELTAEGMESEMKDFAALSEHQTSDSTFLVLMSHGTLHGICGTMHS EKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGEMWIRESSKPQLCRGV DLPRNMEADAVKLSHVEKDFIAFYSTTPHHLSYRDKTGGSYFITRLISCFRKHACSCH LFDIFLKVQQSFEKASIHSQMPTIDRATLTRYFYLFPGN" misc_feature complement(2164..2189) /note="5' UTR of mCASP11" regulatory 2608..2709 /note="ADH1T" /regulatory_class="terminator" CDS complement(2913..4007) /transl_table=11 /note="LEU2" /protein_id="SJL87939.1" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA" rep_origin complement(4672..5074) /note="pMB1 ori" CDS complement(5615..6475) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87940.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7046..8026 /note="2mu ori" BASE COUNT 2220 a 1796 c 1666 g 2344 t ORIGIN 1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga 121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac 181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct 241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga 301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa 421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg 481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt 541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc 601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag 661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc 721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata 781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg 841 cacgagtttt tttttttttt tgggcttgtt tgcttgtcta tggaacagaa tcccactgtg 901 ttgcttaggc tggtcttgaa ttcataagta ttctttcctt aatttcacta gttctggaat 961 ttcaggaata gaatgtgatc cttggccaag atcacttctt caagctgaag aaatgagatt 1021 cagttgcttg ttgctttgtt ctcagttgcc aggaaagagg tagaaatatc tcgtcaaggt 1081 tgcccgatca atggtgggca tctgggaatg aatacttgcc ttttcaaatg attgttgcac 1141 cttcaggaat atatcaaaga gatgacaaga gcaagcatgt ttccggaagc aggaaatgag 1201 tctagtgatg aagtaagagc ctcctgtttt gtctcggtag gacaagtgat gtggggttgt 1261 agagtagaag gcaatgaagt ccttctccac gtggctcagc ttgacagcat cagcttccat 1321 attcctaggt agatctacac ctctgcacaa ctggggtttt gaagactctc tgatccacat 1381 ttctccagag ttcccacctc tgcaggcctg cacaatgatg actttgggtt tgtctcgtag 1441 acctggacag tggcaattgt tgaatatctg atagatggta tcatactgta gcacatctgg 1501 agttttttca ctgtgcattg ttccacaaat gccatgcagt gtgccatgag acattagcac 1561 caggaatgtg ctgtctgatg tctggtgttc tgagagtgca gcaaagtctt tcatctctga 1621 ctccatgccc tctgctgtaa gctcctcttt caccaccaca tcgtagccta agtcttcaag 1681 aaggcctttc ataccaatga tgtcaaagtt agccccatac ctcagtgaga gatgtttgaa 1741 ctctgtattg catatgataa gagcctttcg tgtacggcca ttggcctcct ttattgggta 1801 aatttcttgt gtcttttctc tgcaaagcct tgtgaactct tcaggggaac aaagcttgag 1861 agtgttcaat gattcttctg gttcctccat ttccagatta gcttcaccat ggtggctgcc 1921 tgggtccaca ctgaagaatg tctggagaag catttcacct actttgctgt gtttcttttt 1981 catggcatct acaaaaaccc aacgcttgtc actgcgttca gcattgttaa atttttgttt 2041 atcttcctcc tttaatttca gtacattgct ttgtactaat ttttctaggt actccgtaag 2101 gacttctttg cccagctgtt ccaacacctt aagtggtttg tcagggtgtt tgttttcagc 2161 catgagaaaa agcctcagtt ctttcgcacc gctcgtgccg aattcccggg gatccgtcga 2221 cctgcagaga tctatgaatc gtagatactg aaaaaccccg caagttcact tcaactgtgc 2281 atcgtgcacc atctcaattt ctttcattta tacatcgttt tgccttcttt tatgtaacta 2341 tactcctcta agtttcaatc ttggccatgt aacctctgat ctatagaatt ttttaaatga 2401 ctagaattaa tgcccatctt ttttttggac ctaaattctt catgaaaata tattacgagg 2461 gcttattcag aagctttgga cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt 2521 gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca aatcgttggt 2581 agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat ttttattatt 2641 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa 2701 cgaaaattct tgttcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag 2761 catgaggtcg ctcttattga ccacacctct accggcatgc ccgaaattcc cctaccctat 2821 gaacatattc cattttgtaa tttcgtgtcg tttctattat gaatttcatt tataaagttt 2881 atgtacaaat atcataaaaa aagagaatct ttttaagcaa ggattttctt aacttcttcg 2941 gcgacagcat caccgacttc ggtggtactg ttggaaccac ctaaatcacc agttctgata 3001 cctgcatcca aaaccttttt aactgcatct tcaatggcct taccttcttc aggcaagttc 3061 aatgacaatt tcaacatcat tgcagcagac aagatagtgg cgatagggtc aaccttattc 3121 tttggcaaat ctggagcaga accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg 3181 tctggcaaag aggccaagga cgcagatggc aacaaaccca aggaacctgg gataacggag 3241 gcttcatcgg agatgatatc accaaacatg ttgctggtga ttataatacc atttaggtgg 3301 gttgggttct taactaggat catggcggca gaatcaatca attgatgttg aaccttcaat 3361 gtaggaaatt cgttcttgat ggtttcctcc acagtttttc tccataatct tgaagaggcc 3421 aaaacattag ctttatccaa ggaccaaata ggcaatggtg gctcatgttg tagggccatg 3481 aaagcggcca ttcttgtgat tctttgcact tctggaacgg tgtattgttc actatcccaa 3541 gcgacaccat caccatcgtc ttcctttctc ttaccaaagt aaatacctcc cactaattct 3601 ctgacaacaa cgaagtcagt acctttagca aattgtggct tgattggaga taagtctaaa 3661 agagagtcgg atgcaaagtt acatggtctt aagttggcgt acaattgaag ttctttacgg 3721 atttttagta aaccttgttc aggtctaaca ctacctgtac cccatttagg accacccaca 3781 gcacctaaca aaacggcatc aaccttcttg gaggcttcca gcgcctcatc tggaagtggg 3841 acacctgtag catcgatagc agcaccacca attaaatgat tttcgaaatc gaacttgaca 3901 ttggaacgaa catcagaaat agctttaaga accttaatgg cttcggctgt gatttcttga 3961 ccaacgtggt cacctggcaa aacgacgatc ttcttagggg cagacattag aatggtatat 4021 ccttgaaata tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga 4081 cgattgctaa ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa 4141 gtattgtgat gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc 4201 cggcctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg 4261 cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg cgatagcgcc 4321 cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat tcgaactctt 4381 gcatcttacg atacctgagt attcccacag ttggggatct cgactctagc tagaggatca 4441 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 4501 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac 4561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 4621 tggattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 4681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 4741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 4801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 4861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 4921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 4981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 5041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 5101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 5161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 5221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 5281 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 5341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 5401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 5461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 5521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 5581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 5641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 5701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 5761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 5821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 5881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 5941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 6001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 6061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 6121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 6181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 6241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 6301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 6361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 6421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 6481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 6541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 6601 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 6661 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 6721 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 6781 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 6841 ttgtactgag agtgcaccat aacgcattta agcataaaca cgcactatgc cgttcttctc 6901 atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat 6961 gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct 7021 attccgaagt tcctattctc tagctagaaa gtataggaac ttcagagcgc ttttgaaaac 7081 caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct 7141 actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat 7201 atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat 7261 ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa 7321 ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct 7381 atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga 7441 atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata 7501 taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa 7561 cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc 7621 ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca 7681 caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca 7741 tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg 7801 catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg 7861 ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg 7921 cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc 7981 tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgtt //