LOCUS LT727261 7444 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEGFPC2-haTctn(179-2860), complete
sequence.
ACCESSION LT727261
VERSION LT727261.1
KEYWORDS .
SOURCE Mammalian expression vector pEGFPC2-haTctn(179-2860)
ORGANISM Mammalian expression vector pEGFPC2-haTctn(179-2860)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7444)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7444
/organism="Mammalian expression vector
pEGFPC2-haTctn(179-2860)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4550."
/db_xref="taxon:1945017"
regulatory complement(152..392)
/note="HSV-TK polyA"
/regulatory_class="terminator"
CDS complement(583..1377)
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87921.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin complement(1437..1512)
/note="SV40 ori"
regulatory complement(1469..1750)
/note="SV40 early promoter"
/regulatory_class="promoter"
misc_feature complement(1771..1805)
/note="5' UTR of amp, incl. RBS"
regulatory complement(1806..1901)
/note="ampicillin promoter"
/regulatory_class="promoter"
rep_origin 1902..2357
/note="f1 ori"
regulatory complement(2411..2507)
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory 2411..2507
/note="SV40 polyA late"
/regulatory_class="terminator"
CDS complement(<2667..>5348)
/codon_start=1
/transl_table=11
/note="haTctn"
/protein_id="SJL87922.1"
/translation="SAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPS
SRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEEVRKESEALKV
SAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFETL
KNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLH
SICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSAL
DELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAI
RQALQDLLSEYMNNAGKKERSNTLNIALDNMCKKTRDLRRQLRKAIIDHVSDSFLDTT
VPLLVLIEAAKNGREKEIKEYAAIFHEHTSRLVEVANLACSMSTNEDGIKIVKIAANH
LETLCPQIINAALALAARPKSQAVKNTMEMYKRTWENHIHVLTEAVDDITSIDDFLAV
SESHILEDVNKCIIALRDQDADNLDRAAGAIRGRAARVAHIVTGEMDSYEPGAYTEGV
MRNVNFLTSTVIPEFVTQVNVALEALSKSSLNVLDDNQFVDISKKIYDTIHDIRCSVM
MIRTPEELEDVSDLEEEHEVRSHTSIQTEGKTDRAKMTQLPEAEKEKIAEQVADFKKV
KSKLDAEIEIWDDTSNDIIVLAKNMCMIMMEMTDFTRGKGPLKHTTDVIYAAKMISES
GSRMDVLARQIANQCPDPSCKQDLLAYLEQIKFYSHQLKICSQVKAEIQNLGGELIMS
ALDSVTSLIQAAKNLMNAVVQTVKMSYIASTKIIRIQSPAGPRHPVVMWRMKAPAKKP
LIKREKPEETCAAVRRGSAKKKIHPLQVMSEFRGRQIY"
CDS complement(<5388..6104)
/transl_table=11
/note="EGFP"
/protein_id="SJL87923.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory complement(6149..6716)
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
rep_origin complement(6723..7049)
/note="pMB1 ori"
BASE COUNT 1708 a 1879 c 1863 g 1994 t
ORIGIN
1 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
61 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
121 tatatgagta acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
181 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
241 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
301 tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc
361 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga
421 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga
481 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt
541 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag
601 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
661 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
721 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
781 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
841 gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
901 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
961 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
1021 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
1081 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
1141 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag
1201 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
1261 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
1321 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
1381 aaacgatcct catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc
1441 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
1501 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
1561 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
1621 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
1681 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
1741 cattccacag ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat
1801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
1861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
1921 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
1981 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
2041 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
2101 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
2161 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
2221 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
2281 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
2341 attaacgctt acaatttacg cgttaagata cattgatgag tttggacaaa ccacaactag
2401 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
2461 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
2521 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
2581 tgattatgat cagttatcta gatccggtgg atcccgggcc cgcggtaccg tcgacctgca
2641 ggcggccgca ctagtgattg ggatccgtag atttgtcttc ctctaaattc actcatgact
2701 tgcaatggat ggattttttt ctttgctgag cctcgtctga cagctgcaca cgtttcctct
2761 ggcttctctc ttttaatcaa gggttttttt gcaggagcct tcattctcca catcacaact
2821 gggtgccggg gcccagcagg actctggatt cggatgatct tggttgaggc aatgtaagac
2881 attttcactg tttgcactac agcattcatt aaatttttgg ctgcttggat cagggatgtg
2941 acactgtcca aagctgacat gatgagctct cctcccaggt tctggatctc agctttaact
3001 tgactgcaga ttttcagttg gtgggagtag aacttaatct gttccaggta ggccaacaag
3061 tcctgtttac aagatggatc tgggcactga ttagcaatct gccgagcaag gacatccatc
3121 cttgatcctg attctgatat cattttcgct gcatagatca catcagttgt atgctttagt
3181 ggtcctttgc ccctagtgaa gtctgtcatc tccatcatga tcatacacat gttcttggcc
3241 agaacaatga tgtcgttgct tgtatcatcc catatctcaa tctcagcatc cagcttactc
3301 tttactttct tgaaatcagc aacttgctca gcaatctttt ctttttctgc ctcaggcagt
3361 tgagtcatct tagccctatc agttttccct tcggtctgaa tgctggtgtg actgcggacc
3421 tcgtgttcct cttcaaggtc agaaacatcc tccagttcct ctggggtccg aatcatcatg
3481 actgaacatc tgatatcatg aattgtatca tagatcttct ttgagatgtc cacaaattga
3541 ttatcatcca acacattcaa tgagcttttg cttaaggctt ccaaggcaac attcacttgt
3601 gttacaaatt caggaattac agtacttgta aggaagttaa catttctcat tacaccttcc
3661 gtgtaagccc ctggctcgta actgtccatt tcacccgtga cgatgtgagc aactcttgct
3721 gcccggcctc tgatagcacc cgcagcacgg tctaaattat cagcatcctg gtctcttaag
3781 gctatgatac acttgttgac atcttccaag atatggcttt cagatacagc aaggaagtca
3841 tcaatgcttg taatgtcatc tacggcttca gtgaggacat gtatatgatt ctcccatgta
3901 cgcttgtaca tttccatggt gtttttgacc gcttgacttt tgggtcttgc agccaaagca
3961 agtgcagcat taataatctg tggacacaag gtttccaaat gattggctgc aattttgaca
4021 attttaattc catcttcatt tgttgacatg gaacaagcaa gatttgccac ctctacaagc
4081 ctgctggtgt gttcatgaaa tatcgcagca tattctttta tttccttttc ccggccattc
4141 ttagcagctt caatgagaac caaaagaggg actgtcgtat ccaggaaaga gtctgacaca
4201 tgatctataa tagccttgcg gagctgtctg cgaaggtctc ttgtcttctt acacatgttg
4261 tctaaagcaa tattcagggt attactcctt tctttttttc cagcgttgtt catgtactct
4321 gaaagcagat cctgaagagc ctggcgaatg gcgttgcatt ctgcgataat ccgctctcgg
4381 tgtaagtccc tcgtacatga agaatccgcc agcagagcag ccccactgat aatggcttca
4441 aggcgtttct ctagtgatgg tcgtatttcc tcctcagtta ctgtgagtgg attcaggaca
4501 attaaattct ccagctcatc aagggcactt cccagggttg ctgcctgagg ttctggtggg
4561 gttgtcatat tctggatccc ttgtgaagca tttgaaatta cattgagagc attctgaatt
4621 tcttcacaaa ctgtgtcctt gcttgctttg agggaagcaa catcagaatg ctccaaacaa
4681 gctgaacaaa ttgaatgcaa gaggggagag ttctccttca gtgaagctcg ggctcctgca
4741 atttcatctc tctgatttgg agattttaag tcctgctgac gtttgaaggc taaataatcc
4801 aaattttcca gctcctttcc aagcttctgg taggttttct ggaggtcaga tttgttggca
4861 acatttttga gagtctcaaa tgtcctttga aaagctgaca catgttgcaa gaggcacatg
4921 acatcaatca tgtccgcaag gataaggagt ctcgtcaccg cagccagcaa ggcacgggca
4981 gcttgaacca cagcctccct ttttgggaga aaacaggggt catctgtaaa tctctcagct
5041 gatactttca gagcttcact ttctttgcga acttcctcaa gtgaagccgt aagctcatcc
5101 tttaaaactg tagcttcctg ggcaatcttc tctcccttgt ctaataaatt ccaagttgct
5161 tcctccacag aagctagaag gacactggct cttttcgaac gtcctttttt cctgctggaa
5221 gggttctggg gacagtttac aagtgtggta acctggatta tgagaggctc cagtagcttc
5281 tccacggtga atgtttggac ctgcagatcc tgaggatcga tattcaatgt gattggtgtt
5341 tcagctgaga attcgaagct tgagctcgag atctgagtcc ggccggactt gtacagctcg
5401 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
5461 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
5521 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
5581 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
5641 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
5701 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
5761 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
5821 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
5881 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
5941 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
6001 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
6061 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtagc
6121 gctagcggat ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac
6181 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
6241 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
6301 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
6361 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
6421 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
6481 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
6541 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
6601 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
6661 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactaatgc
6721 atggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
6781 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
6841 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
6901 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
6961 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
7021 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
7081 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
7141 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
7201 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
7261 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
7321 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
7381 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
7441 acgg
//