LOCUS LT727258 5935 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian vector pdEYFP, complete sequence.
ACCESSION LT727258
VERSION LT727258.1
KEYWORDS .
SOURCE Mammalian vector pdEYFP
ORGANISM Mammalian vector pdEYFP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5935)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5935
/organism="Mammalian vector pdEYFP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4547."
/db_xref="taxon:1945178"
regulatory 1..568
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory complement(675..799)
/note="attR1"
/regulatory_class="attenuator"
regulatory 807..859
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 908..1567
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87910.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 1909..2214
/transl_table=11
/note="ccdB"
/protein_id="SJL87911.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 2255..2379
/note="attR2"
/regulatory_class="attenuator"
CDS 2396..3115
/transl_table=11
/note="EYFP"
/protein_id="SJL87912.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory 3216..3312
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3216..3312)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3366..3821)
/note="f1 ori"
regulatory 3822..3917
/note="amp promoter"
/regulatory_class="promoter"
misc_feature 3918..3952
/note="5' UTR of amp, incl. RBS"
regulatory 3973..4254
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4211..4286
/note="SV40 ori"
regulatory 4319..4371
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 4409..4942
/transl_table=11
/note="gentamycin resistance"
/protein_id="SJL87913.1"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP
RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
GIREEVMHFDIDPSTAT"
rep_origin 5603..5929
/note="pMB1 ori"
BASE COUNT 1536 a 1490 c 1460 g 1447 t
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcka attctgcagt cracggtacc gcgggcccgg
661 gatccaccgg atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa
721 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
781 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc
841 cggctcgtat aatgtgtgga ttttgagtta ggatccggcg agattttcag gagctaagga
901 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
961 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
1021 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
1081 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
1141 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
1201 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
1261 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
1321 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
1381 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
1441 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg
1501 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
1561 ggcgtaaaga tctggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
1621 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
1681 tgtgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
1741 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
1801 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
1861 ttattgaaat gaacggctct tttgctgacg agaacaggga ctggtgaaat gcagtttaag
1921 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
1981 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
2041 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
2101 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
2161 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
2221 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
2281 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
2341 attttacgtt tctcgttcag ctttcttgta caaagtggtt gatccggtcg ccaccatggt
2401 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
2461 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
2521 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
2581 gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
2641 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
2701 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
2761 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
2821 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
2881 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
2941 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
3001 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
3061 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg
3121 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
3181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
3241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
3301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
3361 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
3421 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
3481 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
3541 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
3601 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
3661 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
3721 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
3781 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
3841 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
3901 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
3961 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
4021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
4081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
4141 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
4201 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
4261 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgcgca
4321 ttaggcaccc caggctttac actttatgct tcctgctcgt ataatgtgtg gattttgagt
4381 taggatccgg cgaagctaag gagctaaaat gttacgcagc agcaacgatg ttacgcagca
4441 gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gcacatgtag
4501 gctcggccct gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt
4561 cggagacgta gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct
4621 ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct
4681 cgcggcttac gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct
4741 cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa
4801 gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga
4861 tcccgcagtg gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat
4921 cgacccaagt accgccacct aaacgcgtcc accgagaccc cattggggcc aatacgcccg
4981 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
5041 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
5101 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
5161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
5221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
5281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
5341 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
5401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
5461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
5521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
5581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
5641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
5701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
5761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
5821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
5881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//