LOCUS LT727257 9677 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast vector pDEST-GADT7, complete sequence.
ACCESSION LT727257
VERSION LT727257.1
KEYWORDS .
SOURCE Yeast vector pDEST-GADT7
ORGANISM Yeast vector pDEST-GADT7
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9677)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9677
/organism="Yeast vector pDEST-GADT7"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4544."
/db_xref="taxon:1945505"
regulatory 1077..1481
/note="ADH1 promoter"
/regulatory_class="promoter"
CDS 1492..>1899
/transl_table=11
/note="GALad"
/protein_id="SJL87905.1"
/translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE"
regulatory 1905..1921
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 1942..1968
/note="HA epitope"
regulatory complement(1999..2123)
/note="attR1"
/regulatory_class="attenuator"
regulatory 2127..2183
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 2232..2891
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87906.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 3233..3538
/transl_table=11
/note="ccdB"
/protein_id="SJL87907.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 3579..3703
/note="attR2"
/regulatory_class="attenuator"
regulatory 4105..4206
/note="ADH1 terminator"
/regulatory_class="terminator"
CDS complement(4409..5503)
/transl_table=11
/note="LEU2"
/protein_id="SJL87908.1"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
KNTAFGLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
AGIRTGDLGGSNSTTEVGDAVAEEVKKILA"
regulatory complement(5994..6077)
/note="lac promoter"
/regulatory_class="promoter"
misc_feature complement(6120..6153)
/note="loxP"
misc_feature complement(6231..6264)
/note="loxP"
rep_origin complement(6320..6722)
/note="pMB1 ori"
CDS complement(7263..8123)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87909.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 8508..8612
/note="FLP terminator"
/regulatory_class="terminator"
rep_origin 8694..9674
/note="2mu ori"
BASE COUNT 2764 a 2114 c 2090 g 2709 t
ORIGIN
1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
1141 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
1201 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
1261 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
1321 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
1381 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
1441 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa
1501 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgccgcc
1561 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt
1621 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt
1681 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat
1741 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg
1801 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta
1861 ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctttaatac gactcactat
1921 agggcgagcg ccgccatgga gtacccatac gacgtaccag attacgctca tatggccatg
1981 gaggccagtg aattatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
2041 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
2101 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
2161 cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta
2221 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
2281 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
2341 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
2401 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
2461 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
2521 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
2581 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
2641 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
2701 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
2761 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
2821 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
2881 gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc
2941 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
3001 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
3061 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
3121 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
3181 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt
3241 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
3301 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
3361 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
3421 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
3481 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
3541 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
3601 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
3661 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgatgat ccatcgagct
3721 cgagctgcag atgaatcgta gatactgaaa aaccccgcaa gttcacttca actgtgcatc
3781 gtgcaccatc tcaatttctt tcatttatac atcgttttgc cttcttttat gtaactatac
3841 tcctctaagt ttcaatcttg gccatgtaac ctctgatcta tagaattttt taaatgacta
3901 gaattaatgc ccatcttttt tttggaccta aattcttcat gaaaatatat tacgagggct
3961 tattcagaag ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc
4021 ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga
4081 tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
4141 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
4201 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat
4261 gaggtcgctc ttattgacca cacctctacc ggccggtcga aattccccta ccctatgaac
4321 atattccatt ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt
4381 acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga
4441 cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg
4501 catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg
4561 acaatttcaa catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg
4621 gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg
4681 gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgggata acggaggctt
4741 catcggagat gatatcacca aacatgttgc tggtgattat aataccattt aggtgggttg
4801 ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc ttcaatgtag
4861 gaaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa gaggccaaaa
4921 cattagcttt atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag
4981 cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta tcccaagcga
5041 caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact aattctctga
5101 caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag tctaaaagag
5161 agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct ttacggattt
5221 ttagtaaacc ttgttcaggt ctaacactac ctgtacccca tttaggacca cccacagcac
5281 ctaacaaaac ggcatcaacc ttcttggagg cttccagcgc ctcatctgga agtgggacac
5341 ctgtagcgtc gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg
5401 aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa
5461 cgtggtcacc tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt
5521 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat
5581 tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat
5641 tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc
5701 gctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga
5761 cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct
5821 gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca
5881 tcttacgata cctgagtatt cccacagttg gggatctcga ctctagctag aggatcaatt
5941 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
6001 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
6061 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctga
6121 taacttcgta taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag
6181 ccaagctagc ttggctgcag gtcgagcggc cgcgatccgg aacccttaat ataacttcgt
6241 ataatgtatg ctatacgaag ttatcagctg cattaatgaa tcggccaacg cgcggggaga
6301 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
6361 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
6421 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
6481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
6541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
6601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
6661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
6721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
6781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
6841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
6901 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
6961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
7021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
7081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
7141 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
7201 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
7261 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
7321 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
7381 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
7441 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
7501 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
7561 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
7621 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
7681 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
7741 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
7801 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
7861 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
7921 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
7981 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
8041 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
8101 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
8161 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
8221 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
8281 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
8341 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
8401 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
8461 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag
8521 cataaacacg cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata
8581 taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc
8641 tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt
8701 ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa
8761 ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac
8821 attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc
8881 atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat
8941 ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga
9001 aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa
9061 ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag
9121 tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc
9181 acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga
9241 agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag
9301 ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt
9361 gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct
9421 ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt
9481 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag
9541 attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc
9601 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc
9661 acagatgctt cgttgct
//