LOCUS LT727256 8993 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast vector pDEST-GBKT7, complete sequence.
ACCESSION LT727256
VERSION LT727256.1
KEYWORDS .
SOURCE Yeast vector pDEST-GBKT7
ORGANISM Yeast vector pDEST-GBKT7
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8993)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8993
/organism="Yeast vector pDEST-GBKT7"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4543."
/db_xref="taxon:1945506"
regulatory 336..739
/note="ADH1 promoter"
/regulatory_class="promoter"
CDS 762..>1202
/transl_table=11
/note="GALbd"
/protein_id="SJL87900.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
regulatory 1213..1229
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 1251..1280
/note="Myc epitope"
regulatory complement(1308..1432)
/note="attR1"
/regulatory_class="attenuator"
regulatory 1436..1492
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 1541..2200
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87901.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 2542..2847
/transl_table=11
/note="ccdB"
/protein_id="SJL87902.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 2888..3012
/note="attR2"
/regulatory_class="attenuator"
regulatory 3027..3074
/note="T7 terminator"
/regulatory_class="terminator"
regulatory 3101..3202
/note="ADH1 terminator"
/regulatory_class="terminator"
regulatory complement(3355..3438)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(3530..3932)
/note="pMB1 ori"
regulatory complement(4650..4719)
/note="HSV-TK polyA"
/regulatory_class="terminator"
CDS complement(4911..5705)
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87903.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature complement(5706..5740)
/note="5' UTR of amp, incl. RBS"
regulatory complement(5741..5836)
/note="ampicillin promoter"
/regulatory_class="promoter"
rep_origin complement(5837..6817)
/note="2mu ori"
regulatory complement(6895..6999)
/note="FLP terminator"
/regulatory_class="terminator"
CDS 7720..8394
/transl_table=11
/note="TRP1"
/protein_id="SJL87904.1"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK"
rep_origin complement(8497..8952)
/note="f1 ori"
BASE COUNT 2457 a 1988 c 2134 g 2414 t
ORIGIN
1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
1261 tgatctcaga ggaggacctg catatggcca tggaggccga attatcaaca agtttgtaca
1321 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
1381 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
1441 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
1501 ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
1561 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
1621 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
1681 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
1741 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
1801 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
1861 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
1921 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
1981 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
2041 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
2101 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
2161 attacaacag tactgcgatg agtggcaggg cggggcgtaa agatctggat ccggcttact
2221 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
2281 tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg
2341 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
2401 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
2461 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
2521 acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
2581 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
2641 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
2701 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
2761 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
2821 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
2881 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
2941 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
3001 gtacaaagtg gttgatggcc gcataactag cataacccct tggggcctct aaacgggtct
3061 tgaggggttt tttgcgcgct tgcagccaag ctaattccgg gcgaatttct tatgatttat
3121 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
3181 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
3241 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg
3301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
3361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc
3421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
3481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
3541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
3601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
3901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
3961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4141 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
4261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
4321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
4381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
4441 taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg
4501 agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg
4561 tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc
4621 ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc
4681 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg
4741 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg
4801 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat
4861 ggcaggttgg gcgtcgcttg gtcggttcat ttcgaacccc agagtcccgc tcagaagaac
4921 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
4981 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
5041 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
5101 cggccatttt ccaccatgat attcggcaag caggcatcgt catgggtcac gacgagatcc
5161 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
5221 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
5281 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
5341 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
5401 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
5461 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
5521 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
5581 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
5641 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
5701 atcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5761 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5821 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
5881 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
5941 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
6001 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
6061 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
6121 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
6181 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
6241 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
6301 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
6361 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
6421 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
6481 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
6541 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
6601 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
6661 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
6721 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
6781 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
6841 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
6901 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
6961 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
7021 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
7081 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
7141 ctcatccttc aatgctatca tttcctttga tattggatca tattaagaaa ccattattat
7201 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
7261 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
7321 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
7381 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac
7441 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt
7501 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct
7561 tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat
7621 tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg
7681 tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca
7741 ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt
7801 gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca
7861 attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca
7921 ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct
7981 ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa
8041 taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac
8101 atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa
8161 gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa
8221 gagagccccg aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt
8281 gatgcgctta gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat
8341 ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt
8401 actgagtagt atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata
8461 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt
8521 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
8581 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
8641 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
8701 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
8761 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
8821 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
8881 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
8941 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gat
//