LOCUS LT727255 6450 bp DNA circular SYN 06-FEB-2017
DEFINITION Diatom vector pDEST-EGFP, complete sequence.
ACCESSION LT727255
VERSION LT727255.1
KEYWORDS .
SOURCE Diatom vector pDEST-EGFP
ORGANISM Diatom vector pDEST-EGFP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6450)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6450
/organism="Diatom vector pDEST-EGFP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4542."
/db_xref="taxon:1944721"
regulatory 1..568
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
CDS 613..>1329
/transl_table=11
/note="EGFP"
/protein_id="SJL87896.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory complement(1402..1526)
/note="attR1"
/regulatory_class="attenuator"
regulatory 1534..1586
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 1635..2294
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87897.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 2636..2941
/transl_table=11
/note="ccdB"
/protein_id="SJL87898.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 2982..3106
/note="attR2"
/regulatory_class="attenuator"
regulatory 3216..3312
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3216..3312)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3366..3821)
/note="f1 ori"
regulatory 3822..3917
/note="ampicillin resistance promoter"
/regulatory_class="promoter"
misc_feature 3918..3952
/note="5' UTR of amp, incl. RBS"
regulatory 3973..4254
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4211..4286
/note="SV40 ori"
CDS 4346..5140
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87899.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5331..5400
/note="HSV-TK polyA"
/regulatory_class="terminator"
rep_origin 6118..6444
/note="pMB1 ori"
BASE COUNT 1623 a 1636 c 1650 g 1541 t
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg
1381 taccgcgggc ccgggatcat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
1441 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
1501 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
1561 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga ttttcaggag
1621 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
1681 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
1741 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
1801 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
1861 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
1921 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
1981 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
2041 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
2101 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
2161 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct
2221 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
2281 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt
2341 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
2401 aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
2461 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
2521 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
2581 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acagggactg gtgaaatgca
2641 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
2701 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
2761 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
2821 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
2881 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
2941 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
3001 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
3061 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatg atccaccgga
3121 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
3181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
3241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
3301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
3361 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
3421 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
3481 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
3541 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
3601 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
3661 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
3721 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
3781 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
3841 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
3901 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
3961 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
4021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
4081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
4141 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
4201 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
4261 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
4321 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
4381 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
4441 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
4501 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
4561 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
4621 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
4681 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
4741 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
4801 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
4861 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
4921 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
4981 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
5041 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
5101 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
5161 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
5221 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
5281 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
5341 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
5401 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
5461 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
5521 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
5581 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
5641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
5701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
5761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
5821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
5881 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
5941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
6001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
6061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
6121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
6181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
6241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
6301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
6361 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
6421 tctgtggata accgtattac cgccatgcat
//