LOCUS LT727252 7173 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian vector pdcDNA-HA, complete sequence.
ACCESSION LT727252
VERSION LT727252.1
KEYWORDS .
SOURCE Mammalian vector pdcDNA-HA
ORGANISM Mammalian vector pdcDNA-HA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7173)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7173
/organism="Mammalian vector pdcDNA-HA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4536."
/db_xref="taxon:1945172"
regulatory 235..818
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory 863..879
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 957..983
/note="HA epitope"
regulatory complement(987..1111)
/note="attR1"
/regulatory_class="attenuator"
regulatory 1119..1171
/note="lacUV5 promoter"
/regulatory_class="promoter"
CDS 1220..1879
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL87886.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 2221..2526
/transl_table=11
/note="ccdB"
/protein_id="SJL87887.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 2567..2691
/note="attR2"
/regulatory_class="attenuator"
regulatory complement(2728..2744)
/note="SP6 promoter"
/regulatory_class="promoter"
regulatory 2770..2994
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 3040..3468
/note="f1 ori"
regulatory 3473..3754
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3711..3786
/note="SV40 ori"
CDS 3878..4672
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87888.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 4846..4920
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4846..4920)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(5059..5142)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5234..5636)
/note="pMB1 ori"
CDS complement(6177..7037)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87889.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1742 a 1775 c 1791 g 1865 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcatgtacc
961 catacgacgt cccagactac gctatcacaa gtttgtacaa aaaagctgaa cgagaaacgt
1021 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
1081 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
1141 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgg cgagattttc
1201 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
1261 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
1321 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
1381 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
1441 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
1501 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
1561 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
1621 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
1681 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
1741 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
1801 cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
1861 gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc
1921 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
1981 gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat
2041 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
2101 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
2161 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa
2221 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
2281 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
2341 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
2401 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
2461 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
2521 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
2581 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
2641 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tgattcccct
2701 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
2761 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
2821 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
2881 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
2941 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
3001 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
3061 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
3121 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
3181 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc
3241 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
3301 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
3361 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
3421 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
3481 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
3541 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
3601 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
3661 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
3721 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
3781 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
3841 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
3901 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
3961 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
4021 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
4081 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
4141 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
4201 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
4261 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
4321 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
4381 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
4441 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
4501 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
4561 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
4621 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
4681 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
4741 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
4801 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
4861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
4921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
4981 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
5041 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
5101 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
5161 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
5221 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
5281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
5341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
5401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
5461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
5521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
5581 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
5641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
5701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
5761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
5821 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
5881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
5941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
6001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
6061 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
6121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
6181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
6241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
6301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
6361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
6421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
6481 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
6541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
6601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
6661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
6721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
6781 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
6841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
6901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
6961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
7021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
7081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
7141 cgcacatttc cccgaaaagt gccacctgac gtc
//