LOCUS LT727245 6723 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF6-E-hPKR-KD, complete sequence.
ACCESSION LT727245
VERSION LT727245.1
KEYWORDS .
SOURCE Mammalian expression vector pEF6-E-hPKR-KD
ORGANISM Mammalian expression vector pEF6-E-hPKR-KD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6723)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6723
/organism="Mammalian expression vector pEF6-E-hPKR-KD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4511."
/db_xref="taxon:1944991"
regulatory 386..1529
/note="hEF1alpha promoter"
/regulatory_class="promoter"
misc_feature 582..1520
/note="intron"
regulatory 1546..1562
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 1639..1677
/note="E-tag"
CDS 1687..2589
/transl_table=11
/note="hPKR-KD"
/protein_id="SJL87863.1"
/translation="MKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVI
KRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKN
SSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLI
HRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEV
DLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRP
NTSEILRTLTVWKKSPEKNERHTC"
misc_feature 2605..2634
/note="Myc epitope"
misc_feature 2650..2667
/note="His-tag"
regulatory 2696..2920
/note="bovine growth hormone polyA"
/regulatory_class="terminator"
rep_origin 2966..3394
/note="f1 ori"
regulatory 3399..3680
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3637..3712
/note="SV40 ori"
regulatory 3785..3850
/note="EM7 promoter"
/regulatory_class="promoter"
CDS 3851..4249
/transl_table=11
/note="blasticidin"
/protein_id="SJL87864.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
regulatory 4407..4481
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4407..4481)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(4795..5186)
/note="pMB1 ori"
CDS complement(5727..6587)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87865.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1634 a 1625 c 1751 g 1713 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg
1621 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg
1681 gccgctatga aagaaacaaa gtatactgtg gacaagaggt ttggcatgga ttttaaagaa
1741 atagaattaa ttggctcagg tggatttggc caagttttca aagcaaaaca cagaattgac
1801 ggaaagactt acgttattaa acgtgttaaa tataataacg agaaggcgga gcgtgaagta
1861 aaagcattgg caaaacttga tcatgtaaat attgttcact acaatggctg ttgggatgga
1921 tttgattatg atcctgagac cagtgatgat tctcttgaga gcagtgatta tgatcctgag
1981 aacagcaaaa atagttcaag gtcaaagact aagtgccttt tcatccaaat ggaattctgt
2041 gataaaggga ccttggaaca atggattgaa aaaagaagag gcgagaaact agacaaagtt
2101 ttggctttgg aactctttga acaaataaca aaaggggtgg attatataca ttcaaaaaaa
2161 ttaattcata gagatcttaa gccaagtaat atattcttag tagatacaaa acaagtaaag
2221 attggagact ttggacttgt aacatctctg aaaaatgatg gaaagcgaac aaggagtaag
2281 ggaactttgc gatacatgag cccagaacag atttcttcgc aagactatgg aaaggaagtg
2341 gacctctacg ctttggggct aattcttgct gaacttcttc atgtatgtga cactgctttt
2401 gaaacatcaa agtttttcac agacctacgg gatggcatca tctcagatat atttgataaa
2461 aaagaaaaaa ctcttctaca gaaattactc tcaaagaaac ctgaggatcg acctaacaca
2521 tctgaaatac taaggacctt gactgtgtgg aagaaaagcc cagagaaaaa tgaacgacac
2581 acatgttagt ctagagggcc cttcgaacaa aaactcatct cagaagagga tctgaatatg
2641 cataccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
2701 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
2761 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
2821 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
2881 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
2941 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
3001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
3061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3121 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3181 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
3301 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
3361 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
3421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
3481 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
3541 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
3601 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
3661 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
3721 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
3781 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
3841 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
3901 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
3961 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
4021 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
4081 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
4141 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
4201 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg
4261 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
4321 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
4381 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
4441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
4501 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
4561 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
4621 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
4681 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
4741 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
4801 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
4861 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
4921 tgtgagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
4981 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
5041 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
5101 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
5161 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
5221 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
5281 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
5341 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
5401 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
5461 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
5521 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
5581 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
5641 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
5701 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
5761 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
5821 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
5881 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
5941 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
6001 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
6061 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
6121 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
6181 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
6241 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
6301 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
6361 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
6421 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
6481 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
6541 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
6601 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
6661 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
6721 gtc
//