LOCUS LT727240 6716 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF6-eIF2-A-Myc-His, complete sequence.
ACCESSION LT727240
VERSION LT727240.1
KEYWORDS .
SOURCE Mammalian expression vector pEF6-eIF2-A-Myc-His
ORGANISM Mammalian expression vector pEF6-eIF2-A-Myc-His
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6716)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6716
/organism="Mammalian expression vector
pEF6-eIF2-A-Myc-His"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4506."
/db_xref="taxon:1944993"
regulatory 386..1529
/note="hEF1a promoter"
/regulatory_class="promoter"
misc_feature 582..1520
/note="intron hEF1a promoter"
regulatory 1546..1562
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1611..>2555
/transl_table=11
/note="eIF2 alpha subunit"
/protein_id="SJL87848.1"
/translation="MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEG
MILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKF
TKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDP
SILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNC
STENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPK
VVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED"
misc_feature 2556..2567
/note="His4"
misc_feature 2598..2627
/note="Myc epitope"
misc_feature 2643..2660
/note="His-tag"
regulatory 2689..2913
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 2959..3387
/note="f1 ori"
regulatory 3392..3673
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3630..3705
/note="SV40 ori"
regulatory 3778..3843
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 3844..4242
/transl_table=11
/note="blasticidin resistance"
/protein_id="SJL87849.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
regulatory 4400..4474
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4400..4474)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(4613..4696)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4788..5179)
/note="pMB1 ori"
CDS complement(5720..6580)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87850.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1599 a 1630 c 1775 g 1712 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccgt atgccgggtc
1621 taagttgtag attttatcaa cacaaatttc ctgaggtgga agatgtagtg atggtgaatg
1681 tcagatccat tgctgaaatg ggggcttatg tcagcttgct ggaatacaac aacattgaag
1741 gcatgattct tcttagtgaa ttatccagaa ggcgtatccg ttctatcaac aaactcatcc
1801 gaattggcag gaatgagtgt gtggttgtca ttagggtgga caaagaaaaa ggatatattg
1861 atttgtcaaa aagaagagtt tctccagagg aagcaatcaa atgtgaagac aaattcacaa
1921 aatccaaaac tgtttatagc attcttcgtc atgttgctga ggtgttagaa tacaccaagg
1981 atgagcagct ggaaagccta ttccagagga ctgcctgggt ctttgatgac aagtacaaga
2041 gacctggata tggtgcctat gatgcattta agcatgcagt ctcagaccca tctattttgg
2101 atagtttaga tttgaatgaa gatgaacggg aagtactcat taataatatt aataggcgct
2161 tgaccccaca ggctgtcaaa attcgagcag atattgaagt ggcttgttat ggttatgaag
2221 gcattgatgc tgtaaaagaa gccctaagag caggtttgaa ttgttctaca gaaaacatgc
2281 ccattaagat taatctaata gctcctcctc ggtatgtaat gactacgaca accctggaga
2341 gaacagaagg cctttctgtc ctcagtcaag ctatggctgt tatcaaagag aagattgagg
2401 aaaagagggg tgtgttcaat gttcaaatgg agcccaaagt ggtcacagat acagatgaga
2461 ctgaacttgc gaggcagatg gagaggcttg aaagagaaaa tgccgaagtg gatggagatg
2521 atgatgcaga agaaatggaa gccaaagctg aagatcacca ccaccacctg agcggccgcg
2581 actctagagg gcccttcgaa caaaaactca tctcagaaga ggatctgaat atgcataccg
2641 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
2701 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
2761 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
2821 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
2881 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
2941 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
3001 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
3061 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
3121 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
3181 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
3241 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
3301 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
3361 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
3421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
3481 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3541 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
3601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
3661 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
3721 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
3781 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
3841 accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
3901 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
3961 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
4021 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
4081 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
4141 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
4201 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga
4261 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
4321 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
4381 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
4441 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
4501 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
4561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
4621 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
4681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
4741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
4801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
4861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
4921 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
4981 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
5041 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
5101 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
5161 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
5221 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
5281 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
5341 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
5401 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
5461 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
5521 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
5581 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
5641 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
5701 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
5761 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
5821 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
5881 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
5941 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
6001 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
6061 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
6121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
6181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
6241 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
6301 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
6361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
6421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
6481 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
6541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
6601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
6661 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//