LOCUS LT727232 5986 bp DNA circular SYN 06-FEB-2017 DEFINITION Reporter vector p1168hIL6mCRE-C/EBP-luc+, complete sequence. ACCESSION LT727232 VERSION LT727232.1 KEYWORDS . SOURCE Reporter vector p1168hIL6mCRE-C/EBP-luc+ ORGANISM Reporter vector p1168hIL6mCRE-C/EBP-luc+ other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5986) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5986 /organism="Reporter vector p1168hIL6mCRE-C/EBP-luc+" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4497." /db_xref="taxon:1945189" misc_feature 32..672 /note="5' FR of hIL6" regulatory 673..1208 /note="hIL6 promoter mutant" /regulatory_class="promoter" CDS 1256..2908 /transl_table=11 /note="LUCm" /protein_id="SJL87826.1" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" regulatory 2996..3092 /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(2996..3092) /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(3305..3707) /note="pMB1 ori" CDS complement(4248..5108) /transl_table=11 /note="ampicillin" /protein_id="SJL87827.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5240..5695 /note="f1 ori" regulatory complement(5826..5979) /note="synthetic polyA" /regulatory_class="terminator" BASE COUNT 1587 a 1429 c 1433 g 1537 t ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag 61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag 121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt 181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg 241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac 301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag 361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac 421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg 481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc 541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg 601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag 661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga 721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg 781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa 841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac 901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg 961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc 1021 tagttgtgtc ttgcgatgct aaagggatcc acagatatca atcttaataa ggtttccaat 1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat 1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc 1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga 1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc 1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt 1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg 1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag 1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt 1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca 1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa 1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca 1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt 1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac 1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc 1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc 1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt 2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat 2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga 2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga 2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct 2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt 2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct 2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt 2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga 2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat 2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc 2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc 2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt 2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt 2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc 2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc 2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc 3181 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga 3241 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg 3301 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 3361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 3421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 3481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 3541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 3601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 3661 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 3721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 3781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 3841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 3901 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 3961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 4381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 4441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 4501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 4561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 4621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 4681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 4741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 4801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 4861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 4921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 4981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5221 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 5281 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 5341 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 5401 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 5461 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 5521 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 5581 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 5641 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat 5701 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 5761 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg 5821 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga 5881 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg 5941 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata //