LOCUS LT727231 5631 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector p1168hIL6mNFkB-luc+, complete sequence.
ACCESSION LT727231
VERSION LT727231.1
KEYWORDS .
SOURCE Reporter vector p1168hIL6mNFkB-luc+
ORGANISM Reporter vector p1168hIL6mNFkB-luc+
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5631)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5631
/organism="Reporter vector p1168hIL6mNFkB-luc+"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4496."
/db_xref="taxon:1945194"
misc_feature 32..672
/note="5' FR of hIL6"
regulatory 673..1208
/note="hIL6 promoter mutant"
/regulatory_class="promoter"
CDS 1273..2925
/transl_table=11
/note="LUCm"
/protein_id="SJL87824.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 3013..3109
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(3013..3109)
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(3322..3724)
/note="pMB1 ori"
CDS complement(4265..5125)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87825.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5257..5331
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5257..5331)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 5472..5568
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5472..5568)
/note="SV40 polyA late"
/regulatory_class="terminator"
BASE COUNT 1533 a 1319 c 1319 g 1460 t
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatcctcct gcaagagaca ccatcctgag
61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag
121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt
181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg
241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac
301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag
361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac
421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg
481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc
541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg
601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag
661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga
721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg
781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa
841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac
901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg
961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc
1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat
1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgagat
1141 cttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc
1201 tgaggctcat tctgccctcg agatctgcga tctaagtaag cttggcattc cggtactgtt
1261 ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg
1321 ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
1381 cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac
1441 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
1501 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
1561 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
1621 agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt
1681 ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg
1741 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
1801 aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg
1861 aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc
1921 gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
1981 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
2041 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc
2101 cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc
2161 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc
2221 gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc
2281 aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
2341 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
2401 gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg
2461 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
2521 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac
2581 cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc
2641 atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac
2701 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
2761 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
2821 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
2881 gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc
2941 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
3001 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
3061 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
3121 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
3181 atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
3241 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
3301 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
3361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
3421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
3481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
3541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
3601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
3661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
3721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
3781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
3841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
3901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
3961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
4021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
4081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
4141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
4201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
4261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
4321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
4381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
4441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
4501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
4561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
4621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
4681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
4741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
4801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
4861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
4921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
4981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
5041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
5101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
5161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
5221 gggttccgcg cacatttccc cgaaaagtgc cacctgaact tgtttattgc agcttataat
5281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
5341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cataatcagc
5401 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
5461 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
5521 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
5581 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctc g
//