LOCUS LT727229 5986 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector p1168hIL6mGATA-luc+, complete sequence.
ACCESSION LT727229
VERSION LT727229.1
KEYWORDS .
SOURCE Reporter vector p1168hIL6mGATA-luc+
ORGANISM Reporter vector p1168hIL6mGATA-luc+
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5986)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5986
/organism="Reporter vector p1168hIL6mGATA-luc+"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4493."
/db_xref="taxon:1945192"
misc_feature 32..672
/note="5' flanking region of hIL6"
regulatory 673..1208
/note="hIL6 promoter mutant"
/regulatory_class="promoter"
CDS 1256..2908
/transl_table=11
/note="LUCm"
/protein_id="SJL87820.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 2996..3092
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(2996..3092)
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(3305..3707)
/note="pMB1 ori"
CDS complement(4248..5108)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87821.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5240..5695
/note="f1 ori"
regulatory complement(5826..5979)
/note="synthetic polyA"
/regulatory_class="terminator"
BASE COUNT 1586 a 1430 c 1434 g 1536 t
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag
61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag
121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt
181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg
241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac
301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag
361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac
421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg
481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc
541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg
601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag
661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga
721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg
781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa
841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac
901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg
961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc
1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat
1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agattctaga aatgtgggat
1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc
1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga
1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc
2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
3181 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
3241 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
3301 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
3361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
3421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
3481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
3541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
3601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
3661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
3721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
3781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
3841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
3901 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
3961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
4021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
4081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
4141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
4201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
4261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
4321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
4381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
4441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
4501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
4561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
4621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
4681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
4741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
4801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
4861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
4921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
4981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
5041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
5101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
5161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
5221 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
5281 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
5341 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
5401 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
5461 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
5521 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
5581 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
5641 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat
5701 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
5761 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg
5821 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
5881 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
5941 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata
//