LOCUS LT727224 7123 bp DNA circular SYN 06-FEB-2017 DEFINITION Insect expression vector pBlueBachsGCA1, complete sequence. ACCESSION LT727224 VERSION LT727224.1 KEYWORDS . SOURCE Insect expression vector pBlueBachsGCA1 ORGANISM Insect expression vector pBlueBachsGCA1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7123) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7123 /organism="Insect expression vector pBlueBachsGCA1" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4487." /db_xref="taxon:1944735" regulatory 7..95 /note="AcNPV-PH promoter" /regulatory_class="promoter" CDS 191..2263 /transl_table=11 /note="hsGCA1" /protein_id="SJL87806.1" /translation="MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATV PICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKI KESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLK DFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGI IKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLV IPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQT FSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLED FTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKT VDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNA LYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSP HGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYR LLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANF LGKASGID" misc_feature 2292..2333 /note="V5 epitope" misc_feature 2343..2360 /note="His-tag" regulatory 2385..2459 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2385..2459) /note="SV40 polyA late" /regulatory_class="terminator" CDS complement(2647..>3525) /codon_start=1 /transl_table=11 /note="3' AcNPV ORF 1629 fragment" /protein_id="SJL87807.1" /translation="PETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPP MVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSS TTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDVKYVQALFNV FTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLASFVALSENPL DSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPKDYKFNELLK YVQQLSVNQQRTESSA" CDS 3913..4773 /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87808.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5314..5708 /note="pMB1 ori" CDS complement(<5709..6821) /transl_table=11 /note="5' lacZ fragment" /protein_id="SJL87809.1" /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD" regulatory complement(6822..7096) /note="AcNPV-ETL promoter" /regulatory_class="promoter" BASE COUNT 1864 a 1689 c 1712 g 1858 t ORIGIN 1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 181 tgcccttacc atgttctgca cgaagctcaa ggatctcaag atcacaggag agtgtccttt 241 ctccttactg gcaccaggtc aagttcctaa cgagtcttca gaggaggcag caggaagctc 301 agagagctgc aaagcaaccg tgcccatctg tcaagacatt cctgagaaga acatacaaga 361 aagtcttcct caaagaaaaa ccagtcggag ccgagtctat cttcacactt tggcagagag 421 tatttgcaaa ctgattttcc cagagtttga acggctgaat gttgcacttc agagaacatt 481 ggcaaagcac aaaataaaag aaagcaggaa atctttggaa agagaagact ttgaaaaaac 541 aattgcagag caagcagttg cagcaggagt tccagtggag gttatcaaag aatctcttgg 601 tgaagaggtt tttaaaatat gttacgagga agatgaaaac atccttgggg tggttggagg 661 cacccttaaa gattttttaa acagcttcag tacccttctg aaacagagca gccattgcca 721 agaagcagga aaaaggggca ggcttgagga cgcctccatt ctatgcctgg ataaggagga 781 tgattttcta catgtttact acttcttccc taagagaacc acctccctga ttcttcccgg 841 catcataaag gcagctgctc acgtattata tgaaacggaa gtggaagtgt cgttaatgcc 901 tccctgcttc cataatgatt gcagcgagtt tgtgaatcag ccctacttgt tgtactccgt 961 tcacatgaaa agcaccaagc catccctgtc ccccagcaaa ccccagtcct cgctggtgat 1021 tcccacatcg ctattctgca agacatttcc attccatttc atgtttgaca aagatatgac 1081 aattctgcaa tttggcaatg gcatcagaag gctgatgaac aggagagact ttcaaggaaa 1141 gcctaatttt gaagaatact ttgaaattct gactccaaaa atcaaccaga cgtttagcgg 1201 gatcatgact atgttgaata tgcagtttgt tgtacgagtg aggagatggg acaactctgt 1261 gaagaaatct tcaagggtta tggacctcaa aggccaaatg atctacattg ttgaatccag 1321 tgcaatcttg tttttggggt caccctgtgt ggacagatta gaagatttta caggacgagg 1381 gctctacctc tcagacatcc caattcacaa tgcactgagg gatgtggtct taatagggga 1441 acaagcccga gctcaagatg gcctgaagaa gaggctgggg aagctgaagg ctacccttga 1501 gcaagcccac caagccctgg aggaggagaa gaaaaagaca gtagaccttc tgtgctccat 1561 atttccctgt gaggttgctc agcagctgtg gcaagggcaa gttgtgcaag ccaagaagtt 1621 cagtaatgtc accatgctct tctcagacat cgttgggttc actgccatct gctcccagtg 1681 ctcaccgctg caggtcatca ccatgctcaa tgcactgtac actcgcttcg accagcagtg 1741 tggagagctg gatgtctaca aggtggagac cattggcgat gcctattgtg tagctggggg 1801 attacacaaa gagagtgata ctcatgctgt tcagatagcg ctgatggccc tgaagatgat 1861 ggagctctct gatgaagtta tgtctcccca tggagaacct atcaagatgc gaattggact 1921 gcactctgga tcagtttttg ctggcgtcgt tggagttaaa atgccccgtt actgtctttt 1981 tggaaacaat gtcactctgg ctaacaaatt tgagtcctgc agtgtaccac gaaaaatcaa 2041 tgtcagccca acaacttaca gattactcaa agactgtcct ggtttcgtgt ttacccctcg 2101 atcaagggag gaacttccac caaacttccc tagtgaaatc cccggaatct gccattttct 2161 ggatgcttac caacaaggaa caaactcaaa accatgcttc caaaagaaag atgtggaaga 2221 tggcaatgcc aattttttag gcaaagcatc aggaatagat tagcaacaag ggcaattcga 2281 agcttaggcc tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg 2341 gtcatcatca ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag 2401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 2461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc 2521 tgctgaagag gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc 2581 accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta 2641 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt 2701 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt 2761 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt 2821 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt 2881 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt 2941 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt 3001 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc 3061 ctggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca 3121 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt 3181 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc 3241 tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa 3301 cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg 3361 cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg 3421 cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc 3481 tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc 3541 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 3601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 3661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 3721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 3781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 3841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 3901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 3961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 4021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 4081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 4141 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 4201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 4261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 4321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 4381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 4441 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 4501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 4561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 4621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 4681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 4741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 4801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 4861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 5041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 5101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 5161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 5221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 5281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 5341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 5401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 5461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 5521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 5581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 5641 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 5701 gaggaagcat cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc 5761 tcgtgacggt taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt 5821 tcaatccgca cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg 5881 gcggtgtgca gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc 5941 gggttttcga cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata 6001 atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa 6061 actgttaccc gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca 6121 gcgcggctga aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc 6181 ggtttatgca gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct 6241 tccagataac tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg 6301 cgtaaaaatg cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc 6361 cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc 6421 tggccttcct gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga 6481 ttctccgtgg gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg 6541 ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga 6601 agatcgcact ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc 6661 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 6721 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg 6781 ttttcccagt cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat 6841 tgcagctgct ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga 6901 gcgtgcatgt ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat 6961 caacaggctg gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg 7021 catccaatgc aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg 7081 atcgaagcga gtgccttcga ctttttcgtg tccagtgtgg ctt //