LOCUS LT727223 7720 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pAJ401mds1, complete sequence.
ACCESSION LT727223
VERSION LT727223.1
KEYWORDS .
SOURCE Yeast expression vector pAJ401mds1
ORGANISM Yeast expression vector pAJ401mds1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7720)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7720
/organism="Yeast expression vector pAJ401mds1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4485."
/db_xref="taxon:1945442"
regulatory 486..1305
/note="PGK promoter"
/regulatory_class="promoter"
misc_feature 1341..1672
/note="5' UTR mds1"
CDS 1673..3244
/transl_table=11
/note="mds1"
/protein_id="SJL87803.1"
/translation="MRFPSSSVLALGLIGPALAYPKPGATKRGSPNPTRAAAVKAAFQ
TSWNAYHHFAFPHDDLHPVSNSFDDERNGWGSSAIDGLDTAILMGDADIVNTILQYVP
QINFTTTAVANQGSSVFETNIRYLGGLLSAYDLLRGPFSSLATNQTLVNSLLRQAQTL
ANGLKVAFTTPSGVPDPTVFFNPTVRRSGASSNNVAEIGSLVLEWTRLSDLTGNPQYA
QLAQKGESYLLNPKGSPEAWPGLIGTFVSTSNGTFQDSSGSWSGLMDSFYEYLIKMYL
YDPVAFAHYKDRWVLGADSTIGHLGSHPSTRKDLTFLSSYNGQSTSPNSGHLASFGGG
NFILGGILLNEQKYIDFGIKLASSYFGTYTQTASGIGPEGFAWVDSVTGAGGSPPSSQ
SGFYSSAGFWVTAPYYILRPETLESLYYAYRVTGDSKWQDLAWEALSAIEDACRAGSA
YSSINDVTQANGGGASDDMESFWFAEALKYAYLIFAEESDVQVQATGGNKFVFNTEAH
PFSIRSSSRRGGHLA"
misc_feature 3245..3490
/note="3' UTR mds1"
regulatory 3506..3769
/note="PGK terminator"
/regulatory_class="terminator"
regulatory complement(3831..3914)
/note="lac promoter"
/regulatory_class="promoter"
CDS complement(4106..4909)
/transl_table=11
/note="URA3"
/protein_id="SJL87804.1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
rep_origin 5343..5774
/note="2mu ori"
rep_origin complement(5781..6119)
/note="pMB1 ori"
CDS complement(6660..7520)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87805.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1983 a 1897 c 1732 g 2105 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggccgatgcg gccgcaattc
421 gagctcggta cccggggatc ctctagagtc gataaaaaaa aaaaaaaaaa aaaaaaaaaa
481 aaaaaattaa tttttttttc tttcctcttt ttattaacct taatttttat tttagattcc
541 tgacttcaac tcaagacgca cagatattat aacatctgca taataggcat ttgcaagaat
601 tactcgtgag taaggaaaga gtgaggaact atcgcatacc tgcatttaaa gatgccgatt
661 tgggcgcgaa tcctttattt tggcttcacc ctcatactat tatcagggcc agaaaaagga
721 agtgtttccc tccttcttga attgatgtta ccctcataaa gcacgtggcc tcttatcgag
781 aaagaaatta ccgtcgctcg tgatttgttt gcaaaaagaa caaaactgaa aaaacccaga
841 cacgctcgac ttcctgtctt cctattgatt gcagcttcca atttcgtcac acaacaaggt
901 cctagcgacg gctcacaggt tttgtaacaa gcaatcgaag gttctggaat ggcgggaaag
961 ggtttagtac cacatgctat gatgcccact gtgatctcca gagcaaagtt cgttcgatcg
1021 tactgttact ctctctcttt caaacagaat tgtccgaatc gtgtgacaac aacagcctgt
1081 tctcacacac tcttttcttc taaccaaggg ggtggtttag tttagtagaa cctcgtgaaa
1141 cttacattta catatatata aacttgcata aattggtcaa tgcaagaaat agatatttgg
1201 tcttttctaa ttcgtagttt ttcaagttct tagatgcttt ctttttctct tttttacaga
1261 tcatcaagga agtaattatc tactttttac aacaaatata aaaaccaaaa gatctacgta
1321 ctcgaagaat tcggcacgag cgcatctgca gggggcgcca tgtcttaaat tggaagagga
1381 ggaattttat gcttcgcatg aggtacccgc ttggccgctt gaagaacaat tcgcattctg
1441 gtggcgtagg cagtgtcgcg tcgcatgagg gaccccctct tctcattctc ctggctctgc
1501 tgattccgga ggaggaggag gagcatttga gaaagcattg aaatggacgt ctctcccacc
1561 cgtggcactt ttatctgcag ccccgtctca tgccttgagc ctgggcaagt gtctctctcc
1621 tcttcgcctt ggcgtccttg ttgtcaaagc ctgaactaca gctgctgcca acatgagatt
1681 ccctagcagc tccgtccttg ccctcgggct catcggacct gcgctggcgt atccaaagcc
1741 gggcgccaca aaacgtggat ctcccaaccc tacgagggcg gcagcagtca aggccgcatt
1801 ccagacgtcg tggaacgctt accaccattt tgcctttccc catgacgacc tccacccggt
1861 cagcaacagc tttgatgatg agagaaacgg ctggggctcg tcggcaatcg atggcttgga
1921 cacggctatc ctcatggggg atgccgacat tgtgaacacg atccttcagt atgtaccgca
1981 gatcaacttc accacgactg cggttgccaa ccaaggatcc tccgtgttcg agaccaacat
2041 tcggtacctc ggtggcctgc tttctgccta tgacctgttg cgaggtcctt tcagctcctt
2101 ggcgacaaac cagaccctgg taaacagcct tctgaggcag gctcaaacac tggccaacgg
2161 cctcaaggtt gcgttcacca ctcccagcgg tgtcccggac cctaccgtct tcttcaaccc
2221 tactgtccgg agaagtggtg catctagcaa caacgtcgct gaaattggaa gcctggtgct
2281 cgagtggaca cggttgagcg acctgacggg aaacccgcag tatgcccagc ttgcgcagaa
2341 gggcgagtcg tatctcctga atccaaaggg aagcccggag gcatggcctg gcctgattgg
2401 aacgtttgtc agcacgagca acggtacctt tcaggatagc agcggcagct ggtccggcct
2461 catggacagc ttctacgagt acctgatcaa gatgtacctg tacgacccgg ttgcgtttgc
2521 acactacaag gatcgctggg tccttggtgc cgactcgacc attgggcatc tcggctctca
2581 cccgtcgacg cgcaaggact tgaccttttt gtcttcgtac aacggacagt ctacgtcgcc
2641 aaactcagga catttggcca gttttggcgg tggcaacttc atcttgggag gcattctcct
2701 gaacgagcaa aagtacattg actttggaat caagcttgcc agctcgtact ttggcacgta
2761 cacccagacg gcttctggaa tcggccccga aggcttcgcg tgggtggaca gcgtgacggg
2821 cgccggcggc tcgccgccct cgtcccagtc cgggttctac tcgtcggcag gattctgggt
2881 gacggcaccg tattacatcc tgcggccgga gacgctggag agcttgtact acgcataccg
2941 cgtcacgggc gactccaagt ggcaggacct ggcgtgggaa gcgttgagtg ccattgagga
3001 cgcatgccgc gccggcagcg cgtactcgtc catcaacgac gtgacgcagg ccaacggcgg
3061 gggtgcctct gacgatatgg agagcttctg gtttgccgag gcgctcaagt atgcgtacct
3121 gatctttgcg gaggagtcgg atgtgcaggt gcaggccacc ggcgggaaca aatttgtctt
3181 taacacggag gcgcacccct ttagcatccg ttcatcatca cgacggggcg gccaccttgc
3241 ttaaaggcgc tgagagttct ctcgcctgtt tttttgctcg tattattcat tttctgctgc
3301 tcgtcccgaa tcgctccgta gatattttga cacatgcgtc tggatagagc ggctgggacc
3361 accgaatatg gtatgtcatt cgagatatga agcgcaagag atctttaaga tccaagttct
3421 ctggtattat actggatgag atagatctat aggaaaccct gtccgaattg tgtggaaaaa
3481 aaaaaaaaaa nnnctcgagt aattccgata gatcaatttt tttcttttct ctttccccat
3541 cctttacgct aaaataatag tttattttat tttttgaata ttttttattt atatacgtat
3601 atatagacta ttatttactt ttaatagatt attaagattt ttattaaaaa aaaattcgtc
3661 cctcttttta atgcctttta tgcagttttt ttttcccatt cgatatttct atgttcgggt
3721 ttcagcgtat tttaagttta ataactcgaa aattctgcgt ttcgaaaaaa agcttggcgt
3781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
3841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
3901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcagatct
3961 gagctttttc tttccaattt tttttttttc gtcattataa aaatcattac gaccgagatt
4021 cccggccggg taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac
4081 atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt
4141 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata
4201 gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat
4261 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc
4321 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat
4381 ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc
4441 ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg
4501 ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt
4561 ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa
4621 tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta
4681 gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta
4741 aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat
4801 ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa
4861 caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc
4921 gtttccggtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc
4981 aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt
5041 ctgttcggag attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata
5101 aaaaaaaaat gatgaattga attgaaaagc tcagatctgc tgcattaatg aatcggccaa
5161 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
5221 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagcat cgatggcagc tcgcgttgca
5281 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct
5341 ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg aagacgcact
5401 ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc gaataccgct
5461 tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat
5521 aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt
5581 tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga
5641 gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa
5701 tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg
5761 ttcacaaagt atgcatcgat gctcactcaa aggtcggtaa tacggttatc cacagaatca
5821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
5881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
5941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
6361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
6481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
6541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
6601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
6661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
6721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
6781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
6841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
6901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
6961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
7021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
7081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
7141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
7201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
7261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
7321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
7381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
7441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
7501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
7561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
7621 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
7681 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//