LOCUS LT727222 9502 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pPP1mds1, complete sequence.
ACCESSION LT727222
VERSION LT727222.1
KEYWORDS .
SOURCE Yeast expression vector pPP1mds1
ORGANISM Yeast expression vector pPP1mds1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9502)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9502
/organism="Yeast expression vector pPP1mds1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4484."
/db_xref="taxon:1945494"
regulatory 7..772
/note="AOX1 promoter"
/regulatory_class="promoter"
misc_feature 773..937
/note="5' UTR of AOX1"
CDS 954..2525
/transl_table=11
/note="mds1"
/protein_id="SJL87800.1"
/translation="MRFPSSSVLALGLIGPALAYPKPGATKRGSPNPTRAAAVKAAFQ
TSWNAYHHFAFPHDDLHPVSNSFDDERNGWGSSAIDGLDTAILMGDADIVNTILQYVP
QINFTTTAVANQGSSVFETNIRYLGGLLSAYDLLRGPFSSLATNQTLVNSLLRQAQTL
ANGLKVAFTTPSGVPDPTVFFNPTVRRSGASSNNVAEIGSLVLEWTRLSDLTGNPQYA
QLAQKGESYLLNPKGSPEAWPGLIGTFVSTSNGTFQDSSGSWSGLMDSFYEYLIKMYL
YDPVAFAHYKDRWVLGADSTIGHLGSHPSTRKDLTFLSSYNGQSTSPNSGHLASFGGG
NFILGGILLNEQKYIDFGIKLASSYFGTYTQTASGIGPEGFAWVDSVTGAGGSPPSSQ
SGFYSSAGFWVTAPYYILRPETLESLYYAYRVTGDSKWQDLAWEALSAIEDACRAGSA
YSSINDVTQANGGGASDDMESFWFAEALKYAYLIFAEESDVQVQATGGNKFVFNTEAH
PFSIRSSSRRGGHLA"
regulatory 2800..3059
/note="AOX1 terminator"
/regulatory_class="terminator"
CDS complement(3459..5993)
/transl_table=11
/note="HIS4"
/protein_id="SJL87801.1"
/translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST
ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR
TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII
PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS
KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF
CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE
AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ
PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ
NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLT
ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP
PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA
AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAV
DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI
LQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQ
KFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
misc_feature 6348..7105
/note="3' flanking region of AOX1"
rep_origin complement(7389..7791)
/note="pMB1 ori"
CDS complement(8332..9192)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87802.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2418 a 2358 c 2245 g 2481 t
ORIGIN
1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
901 caacttgaga agatcaaaaa acaactaatt attcgaagga tcctgctgcc aacatgagat
961 tccctagcag ctccgtcctt gccctcgggc tcatcggacc tgcgctggcg tatccaaagc
1021 cgggcgccac aaaacgtgga tctcccaacc ctacgagggc ggcagcagtc aaggccgcat
1081 tccagacgtc gtggaacgct taccaccatt ttgcctttcc ccatgacgac ctccacccgg
1141 tcagcaacag ctttgatgat gagagaaacg gctggggctc gtcggcaatc gatggcttgg
1201 acacggctat cctcatgggg gatgccgaca ttgtgaacac gatccttcag tatgtaccgc
1261 agatcaactt caccacgact gcggttgcca accaaggatc ctccgtgttc gagaccaaca
1321 ttcggtacct cggtggcctg ctttctgcct atgacctgtt gcgaggtcct ttcagctcct
1381 tggcgacaaa ccagaccctg gtaaacagcc ttctgaggca ggctcaaaca ctggccaacg
1441 gcctcaaggt tgcgttcacc actcccagcg gtgtcccgga ccctaccgtc ttcttcaacc
1501 ctactgtccg gagaagtggt gcatctagca acaacgtcgc tgaaattgga agcctggtgc
1561 tcgagtggac acggttgagc gacctgacgg gaaacccgca gtatgcccag cttgcgcaga
1621 agggcgagtc gtatctcctg aatccaaagg gaagcccgga ggcatggcct ggcctgattg
1681 gaacgtttgt cagcacgagc aacggtacct ttcaggatag cagcggcagc tggtccggcc
1741 tcatggacag cttctacgag tacctgatca agatgtacct gtacgacccg gttgcgtttg
1801 cacactacaa ggatcgctgg gtccttggtg ccgactcgac cattgggcat ctcggctctc
1861 acccgtcgac gcgcaaggac ttgacctttt tgtcttcgta caacggacag tctacgtcgc
1921 caaactcagg acatttggcc agttttggcg gtggcaactt catcttggga ggcattctcc
1981 tgaacgagca aaagtacatt gactttggaa tcaagcttgc cagctcgtac tttggcacgt
2041 acacccagac ggcttctgga atcggccccg aaggcttcgc gtgggtggac agcgtgacgg
2101 gcgccggcgg ctcgccgccc tcgtcccagt ccgggttcta ctcgtcggca ggattctggg
2161 tgacggcacc gtattacatc ctgcggccgg agacgctgga gagcttgtac tacgcatacc
2221 gcgtcacggg cgactccaag tggcaggacc tggcgtggga agcgttgagt gccattgagg
2281 acgcatgccg cgccggcagc gcgtactcgt ccatcaacga cgtgacgcag gccaacggcg
2341 ggggtgcctc tgacgatatg gagagcttct ggtttgccga ggcgctcaag tatgcgtacc
2401 tgatctttgc ggaggagtcg gatgtgcagg tgcaggccac cggcgggaac aaatttgtct
2461 ttaacacgga ggcgcacccc tttagcatcc gttcatcatc acgacggggc ggccaccttg
2521 cttaaaggcg ctgagagttc tctcgcctgt ttttttgctc gtattattca ttttctgctg
2581 ctcgtcccga atcgctccgt agatattttg acacatgcgt ctggatagag cggctgggac
2641 caccgaatat ggtatgtcat tcgagatatg aagcgcaaga gatccccggg taccgagctc
2701 gaattcccta gggcggccgc gaattaattc gccttagaca tgactgttcc tcagttcaag
2761 ttgggcactt acgagaagac cggtcttgct agattctaat caagaggatg tcagaatgcc
2821 atttgcctga gagatgcagg cttcattttt gatacttttt tatttgtaac ctatatagta
2881 taggattttt tttgtcattt tgtttcttct cgtacgagct tgctcctgat cagcctatct
2941 cgcagctgat gaatatcttg tggtaggggt ttgggaaaat cattcgagtt tgatgttttt
3001 cttggtattt cccactcctc ttcagagtac agaagattaa gtgagaagtt cgtttgtgca
3061 agcttatcga taagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg
3121 caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg
3181 ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt
3241 ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc
3301 tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg
3361 cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga
3421 tctatcgaat ctaaatgtaa gttaaaatct ctaaataatt aaataagtcc cagtttctcc
3481 atacgaacct taacagcatt gcggtgagca tctagacctt caacagcagc cagatccatc
3541 actgcttggc caatatgttt cagtccctca ggagttacgt cttgtgaagt gatgaacttc
3601 tggaaggttg cagtgttaac tccgctgtat tgacgggcat atccgtacgt tggcaaagtg
3661 tggttggtac cggaggagta atctccacaa ctctctggag agtaggcacc aacaaacaca
3721 gatccagcgt gttgtacttg atcaacataa gaagaagcat tctcgatttg caggatcaag
3781 tgttcaggag cgtactgatt ggacatttcc aaagcctgct cgtaggttgc aaccgatagg
3841 gttgtagagt gtgcaataca cttgcgtaca atttcaaccc ttggcaactg cacagcttgg
3901 ttgtgaacag catcttcaat tctggcaagc tccttgtctg tcatatcgac agccaacaga
3961 atcacctggg aatcaatacc atgttcagct tgagacagaa ggtctgaggc aacgaaatct
4021 ggatcagcgt atttatcagc aataactaga acttcagaag gcccagcagg catgtcaata
4081 ctacacaggg ctgatgtgtc attttgaacc atcatcttgg cagcagtaac gaactggttt
4141 cctggaccaa atattttgtc acacttagga acagtttctg ttccgtaagc catagcagct
4201 actgcctggg cgcctcctgc tagcacgata cacttagcac caaccttgtg ggcaacgtag
4261 atgacttctg gggtaagggt accatccttc ttaggtggag atgcaaaaac aatttctttg
4321 caaccagcaa ctttggcagg aacacccagc atcagggaag tggaaggcag aattgcggtt
4381 ccaccaggaa tatagaggcc aactttctca ataggtcttg caaaacgaga gcagactaca
4441 ccagggcaag tctcaacttg caacgtctcc gttagttgag cttcatggaa tttcctgacg
4501 ttatctatag agagatcaat ggctctctta acgttatctg gcaattgcat aagttcctct
4561 gggaaaggag cttctaacac aggtgtcttc aaagcgactc catcaaactt ggcagttagt
4621 tctaaaaggg ctttgtcacc attttgacga acattgtcga caattggttt gactaattcc
4681 ataatctgtt ccgttttctg gataggacga cgaagggcat cttcaatttc ttgtgaggag
4741 gccttagaaa cgtcaatttt gcacaattca atacgacctt cagaagggac ttctttaggt
4801 ttggattctt ctttaggttg ttccttggtg tatcctggct tggcatctcc tttccttcta
4861 gtgaccttta gggacttcat atccaggttt ctctccacct cgtccaacgt cacaccgtac
4921 ttggcacatc taactaatgc aaaataaaat aagtcagcac attcccaggc tatatcttcc
4981 ttggatttag cttctgcaag ttcatcagct tcctccctaa ttttagcgtt caacaaaact
5041 tcgtcgtcaa ataaccgttt ggtataagaa ccttctggag cattgctctt acgatcccac
5101 aaggtggctt ccatggctct aagacccttt gattggccaa aacaggaagt gcgttccaag
5161 tgacagaaac caacacctgt ttgttcaacc acaaatttca agcagtctcc atcacaatcc
5221 aattcgatac ccagcaactt ttgagttgct ccagatgtag cacctttata ccacaaaccg
5281 tgacgacgag attggtagac tccagtttgt gtccttatag cctccggaat agactttttg
5341 gacgagtaca ccaggcccaa cgagtaatta gaagagtcag ccaccaaagt agtgaataga
5401 ccatcggggc ggtcagtagt caaagacgcc aacaaaattt cactgacagg gaactttttg
5461 acatcttcag aaagttcgta ttcagtagtc aattgccgag catcaataat ggggattata
5521 ccagaagcaa cagtggaagt cacatctacc aactttgcgg tctcagaaaa agcataaaca
5581 gttctactac cgccattagt gaaacttttc aaatcgccca gtggagaaga aaaaggcaca
5641 gcgatactag cattagcggg caaggatgca actttatcaa ccagggtcct atagataacc
5701 ctagcgcctg ggatcatcct ttggacaact ctttctgcca aatctaggtc caaaatcact
5761 tcattgatac cattattgta caacttgagc aagttgtcga tcagctcctc aaattggtcc
5821 tctgtaacgg atgactcaac ttgcacatta acttgaagct cagtcgattg agtgaacttg
5881 atcaggttgt gcagctggtc agcagcatag ggaaacacgg cttttcctac caaactcaag
5941 gaattatcaa actctgcaac acttgcgtat gcaggtagca agggaaatgt catacttgaa
6001 gtcggacagt gagtgtagtc ttgagaaatt ctgaagccgt atttttatta tcagtgagtc
6061 agtcatcagg agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
6121 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
6181 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
6241 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
6301 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agtatctatg
6361 attggaagta tgggaatggt gatacccgca ttcttcagtg tcttgaggtc tcctatcaga
6421 ttatgcccaa ctaaagcaac cggaggagga gatttcatgg taaatttctc tgacttttgg
6481 tcatcagtag actcgaactg tgagactatc tcggttatga cagcagaaat gtccttcttg
6541 gagacagtaa atgaagtccc accaataaag aaatccttgt tatcaggaac aaacttcttg
6601 tttcgaactt tttcggtgcc ttgaactata aaatgtagag tggatatgtc gggtaggaat
6661 ggagcgggca aatgcttacc ttctggacct tcaagaggta tgtagggttt gtagatactg
6721 atgccaactt cagtgacaac gttgctattt cgttcaaacc attccgaatc cagagaaatc
6781 aaagttgttt gtctactatt gatccaagcc agtgcggtct tgaaactgac aatagtgtgc
6841 tcgtgttttg aggtcatctt tgtatgaata aatctagtct ttgatctaaa taatcttgac
6901 gagccaaggc gataaatacc caaatctaaa actcttttaa aacgttaaaa ggacaagtat
6961 gtctgcctgt attaaacccc aaatcagctc gtagtctgat cctcatcaac ttgaggggca
7021 ctatcttgtt ttagagaaat ttgcggagat gcgatatcga gaaaaaggta cgctgatttt
7081 aaacgtgaaa tttatctcaa gatctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
7141 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
7201 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
7261 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
7321 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
7381 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
7441 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
7501 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
7561 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
7621 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
7681 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
7741 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
7801 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
7861 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
7921 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
7981 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
8041 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
8101 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
8161 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
8221 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
8281 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
8341 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
8401 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
8461 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
8521 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
8581 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
8641 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
8701 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
8761 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
8821 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
8881 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
8941 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
9001 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
9061 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
9121 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
9181 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
9241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
9301 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
9361 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttaattctca tgtttgacag
9421 cttatcatcg ataagctgac tcatgttggt attgtgaaat agacgcagat cgggaacact
9481 gaaaaataac agttattatt cg
//