LOCUS LT727221 6738 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAhIFNGSTm, complete sequence.
ACCESSION LT727221
VERSION LT727221.1
KEYWORDS .
SOURCE Mammalian expression vector pCAhIFNGSTm
ORGANISM Mammalian expression vector pCAhIFNGSTm
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6738)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6738
/organism="Mammalian expression vector pCAhIFNGSTm"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4482."
/db_xref="taxon:1944900"
CDS 69..>137
/transl_table=11
/note="hIFNG signal sequence"
/protein_id="SJL87797.1"
/translation="MKYTSYILAFQLCIVLGSLGCYC"
CDS <149..1211
/codon_start=2
/transl_table=11
/note="mature hST6GalI mutant"
/protein_id="SJL87798.1"
/translation="AAMGSDSQSVSSSSTQDPHRGRQTLGSLRGLAKAKPEASFQVWN
KDSSSKNLIPRLQKIWKNYLSMNKYKVSYKGPGPGIKFSAEALRCHLRDHVNVSMVEV
TDFPFNTSEWEGYLPKESIRTKAGPWGRCAVVSSAGSLKSSQLGREIDDHDAVLRFNG
APTANFQQDVGTKTTIRLMNSQLVTTEKRFLKDSLYNEGILIVWDPSVYHSDIPKWYQ
NPDYNFFNNYKTYRKLHPNQPFYILKPQMPWELWDILQEISPEEIQPNPPSSGMLGII
IMMTLCDQVDIYEFLPSKRKTDVCYYYQKFFDSACTMGAYHPLLYEKNLVKHLNQGTD
EDIYLLGKATLPGFRTIHC"
regulatory 2028..2119
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 2120..2476
/note="3' FR of beta-globin"
regulatory complement(2554..2636)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2778..2853
/note="SV40 ori"
regulatory 2884..2958
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2884..2958)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3073..3474)
/note="pMB1 ori"
CDS complement(4015..4875)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87799.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5011..5388
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5394..6735
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5763..6687
/note="intron"
BASE COUNT 1584 a 1722 c 1741 g 1691 t
ORIGIN
1 ttcctcgagg tcagcttgat acaagaacta ctgatttcaa cttctttggc ttaattctct
61 cggaaacgat gaaatataca agttatatct tggcttttca gctctgcatc gttttgggtt
121 ctcttggctg ttactgccag gacccagcgg ccgccatggg gtctgattcc cagtctgtat
181 cctcaagcag cacccaggac ccccacaggg gccgccagac cctcggcagt ctcagaggcc
241 tagccaaggc caaaccagag gcctccttcc aggtgtggaa caaggacagc tcttccaaaa
301 accttatccc taggctgcaa aagatctgga agaattacct aagcatgaac aagtacaaag
361 tgtcctacaa ggggccagga ccaggcatca agttcagtgc agaggccctg cgctgccacc
421 tccgggacca tgtgaatgta tccatggtag aggtcacaga ttttcccttc aatacctctg
481 aatgggaggg ttatctgccc aaggagagca ttaggaccaa ggctgggcct tggggcaggt
541 gtgctgttgt gtcgtcagcg ggatctctga agtcctccca actaggcaga gaaatcgatg
601 atcatgacgc agtcctgagg tttaatgggg cacccacagc caacttccaa caagatgtgg
661 gcacaaaaac taccattcgc ctgatgaact ctcagttggt taccacagag aagcgcttcc
721 tcaaagacag tttgtacaat gaaggaatcc taattgtatg ggacccatct gtataccact
781 cagatatccc aaagtggtac cagaatccgg attataattt ctttaacaac tacaagactt
841 atcgtaagct gcaccccaat cagccctttt acatcctcaa gccccagatg ccttgggagc
901 tatgggacat tcttcaagaa atctccccag aagagattca gccaaacccc ccatcctctg
961 ggatgcttgg tatcatcatc atgatgacgc tgtgtgacca ggtggatatt tatgagttcc
1021 tcccatccaa gcgcaagact gacgtgtgct actactacca gaagttcttc gatagtgcct
1081 gcacgatggg tgcctaccac ccgctgctct atgagaagaa tttggtgaag catctcaacc
1141 agggcacaga tgaggacatc tacctgcttg gaaaagccac actgcctggc ttccggacca
1201 ttcactgcta agcacaggct cctcactctt ctccatcagg cattaaatga atggtctctt
1261 ggcctatgta aaagaagcag aaaaccttaa gaaatatttt aatgcaggtc attcagatgt
1321 agcggataat ggaactcttt tcttaggcat tttgaagaat tggaaagagg agagtgacag
1381 aaaaataatg cagagccaaa ttgtctcctt ttacttcaaa ctttttaaaa actttaaaga
1441 tgaccagagc atccaaaaga gtgtggagac catcaaggaa gacatgaatg tcaagttttt
1501 caatagcaac aaaaagaaac gagatgactt cgaaaagctg actaattatt cggtaactga
1561 cttgaatgtc caacgcaaag caatacatga actcatccaa gtgatggctg aactgtcgcc
1621 agcagctaaa acagggaagc gaaaaaggag tcagatgctg tttcgaggtc gaagagcatc
1681 ccagtaatgg ttgtcctgcc tgcaatattt gaattttaaa tctaaatcta tttattaata
1741 tttaacatta tttatatggg gaatatattt ttagactcat caatcaaata agtatttata
1801 atagcaactt ttgtgtaatg aaaatgaata tctattaata tatgtattat ttataattcc
1861 tatatcctgt gactgtctca cttaatcctt tgttttctga ctaattaggc aaggctatgt
1921 gattacaagg ctttatctca ggggccaact aggcagccaa cctaagcaag acctaggctt
1981 ttgcaaaaag ctcaagctcc aatgccctgg ctcacaaata ccactgagat ctttttccct
2041 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
2101 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
2161 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
2221 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
2281 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
2341 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
2401 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
2461 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
2521 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
2581 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
2641 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
2701 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
2761 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
2821 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
2881 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
2941 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
3001 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
3061 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
3121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga
3181 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
3241 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3301 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3361 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3421 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3481 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3541 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3601 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
3661 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
3721 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3781 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
3901 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
3961 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
4021 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
4081 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
4141 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
4201 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
4261 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
4321 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
4381 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
4441 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
4501 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
4561 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
4621 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
4681 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
4741 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4801 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4861 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
4921 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
4981 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
5041 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
5101 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
5161 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
5221 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
5281 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
5341 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
5401 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
5461 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc
5521 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag
5581 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
5641 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc
5701 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
5761 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
5821 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
5881 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
5941 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
6001 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
6061 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
6121 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
6181 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
6241 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
6301 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
6361 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
6421 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
6481 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
6541 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
6601 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
6661 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
6721 catcattttg gcaaagaa
//