LOCUS LT727218 7173 bp DNA circular SYN 06-FEB-2017
DEFINITION Insect expression vector pBlueBacmsGCA1, complete sequence.
ACCESSION LT727218
VERSION LT727218.1
KEYWORDS .
SOURCE Insect expression vector pBlueBacmsGCA1
ORGANISM Insect expression vector pBlueBacmsGCA1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7173)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7173
/organism="Insect expression vector pBlueBacmsGCA1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4471."
/db_xref="taxon:1944736"
regulatory 7..95
/note="AcNPV-PH promoter"
/regulatory_class="promoter"
CDS 213..2288
/transl_table=11
/note="msGCA1"
/protein_id="SJL87791.1"
/translation="MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATL
PICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKI
EENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLK
DFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGI
IKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLV
IPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQT
FSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLED
FTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRT
VDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNA
LYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSP
HGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYR
LLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNAN
FLGKASGVD"
misc_feature 2342..2383
/note="V5 epitope"
misc_feature 2393..2410
/note="His-tag"
regulatory 2435..2509
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2435..2509)
/note="SV40 polyA late"
/regulatory_class="terminator"
CDS complement(2697..>3575)
/codon_start=1
/transl_table=11
/note="3' AcNPV ORF 1629"
/protein_id="SJL87792.1"
/translation="PETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPP
MVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSS
TTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDVKYVQALFNV
FTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLASFVALSENPL
DSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPKDYKFNELLK
YVQQLSVNQQRTESSA"
CDS 3963..4823
/transl_table=11
/note="ampicillin"
/protein_id="SJL87793.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5364..5758
/note="pMB1 ori"
CDS complement(<5759..6871)
/transl_table=11
/note="5' lacZ fragment"
/protein_id="SJL87794.1"
/translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS
QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP
ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD
SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE
NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
regulatory complement(6872..7146)
/note="AcNPV-ETL promoter"
/regulatory_class="promoter"
BASE COUNT 1806 a 1756 c 1779 g 1832 t
ORIGIN
1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
181 tgcccttgcc actgccaagc tccaggaaca ccatgttctg caggaagttc aaggatctca
241 agatcacagg ggagtgtcct ttctccttac tggcccctgg tcaggttcct aaggagccaa
301 cagaggaggt ggctggaggc tctgagggct gccaggctac tctgcccatc tgccagtact
361 ttcctgagaa gaatgcagaa gggagtctcc cccaaagaaa gacaagccgc aacagagtct
421 acctgcacac cctggcagag agtatttgca agctcatctt cccagagtgt gagcgactga
481 accttgcact tcagagaacc ttggcaaagc ataaaataga agaaaacagg aaatcttcag
541 aaaaagaaga ccttgaaaaa ataatcgcag aagaagcaat tgcagcaggt gccccagtgg
601 aggcgctcaa agactctctg ggcgaggagc tgttcaagat ctgctatgag gaagatgagc
661 acattttggg cgtggttggc ggcaccctga aggactttct aaatagcttc agcacgctcc
721 tcaagcagag cagccactgc caagaggcgg agaggcgggg acgactggaa gatgcctcca
781 tcttatgcct ggacaaggac caggactttc taaatgttta ctacttcttc ccgaagagaa
841 ccacagccct gcttctccct ggtatcatta aagcggctgc tcgcatactg tacgaaagcc
901 acgtggaggt gtccctgatg cctccctgct tccgaagtga ctgtaccgag tttgtgaacc
961 agccctattt gctctactcc gttcatgtga agagcaccaa gccgtccctg tccccaggca
1021 agccccagtc ctcgctagtg atccccgctt cgctcttctg caagactttc ccgttccatt
1081 tcatgctgga ccgagacctg gccatcctgc agctgggtaa cggcatcaga aggctggtga
1141 acaagaggga cttccaaggg aagcccaact ttgaagagtt ctttgaaatt ctaactccca
1201 aaatcaacca gacatttagt ggcatcatga caatgttgaa catgcagttt gtcatccggg
1261 tgaggagatg ggataactcg gtgaagaaat cgtcaagggt tatggatctc aaaggtcaaa
1321 tgatctacat cgttgaatcc agtgccatct tgttcttagg gtcaccatgt gtggacaggc
1381 tggaagattt cacaggacgg gggctctatc tgtccgacat cccaattcat aacgccctga
1441 gggatgttgt cttgataggg gagcaggcac gggctcaaga tggcctcaag aagaggttgg
1501 ggaagctgaa ggcaaccctg gagcatgccc accaagccct ggaggaagag aagaagagga
1561 cagtggatct gctgtgctct atcttcccct ctgaggttgc tcagcagctg tggcaaggac
1621 aaattgtgca agccaagaaa ttcagcgagg tcaccatgct tttctcagat atcgtagggt
1681 tcactgctat ctgctctcag tgttcacctc tgcaggtcat cacgatgctc aacgctctct
1741 acactcgctt tgaccagcag tgtggagagc tggatgtcta caaggtggag accatcgggg
1801 atgcatattg tgtggcaggt ggattgcaca gagagagtga cacccatgct gtccagatag
1861 cactgatggc cctgaagatg atggagctct ccaatgaggt catgtctccc cacggagaac
1921 ctatcaagat gcgaattgga ctacattctg gatcagtgtt tgctggagtt gtcggagtga
1981 agatgccccg gtattgcctg tttggaaaca atgtcactct ggctaacaaa tttgaatcct
2041 gcagtgtgcc tcggaaaatc aatgtcagcc ccaccacata caggttactc aaagactgtc
2101 ctggctttgt gttcaccccg agatcaaggg aggagcttcc accaaacttc cctagtgaca
2161 ttcctgggat ctgtcacttt ctggatgctt atcaccatca aggacctaat tccaaaccat
2221 ggttccagga taaagatgtg gaagatggaa acgccaactt cttaggcaaa gcgtcagggg
2281 tagattagtg agccacatgc tcttatgttt gatgcctaag ggcaattcga agcttaggcc
2341 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
2401 ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag cttataatgg
2461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
2521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc tgctgaagag
2581 gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc accagacgca
2641 cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta catttattaa
2701 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca
2761 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct
2821 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt
2881 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac
2941 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
3001 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
3061 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ctggacatat
3121 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
3181 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg
3241 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg
3301 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa
3361 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt
3421 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga
3481 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac
3541 acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc agtacaatct
3601 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
3661 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
3721 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
3781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
3841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
3901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
3961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
4021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
4081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
4141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
4201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
4261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
4321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
4381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
4441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
4501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
4561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
4621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
4681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
4741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
4801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
4861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
4921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
4981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
5041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
5101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
5161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
5221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
5281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
5341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
5401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
5461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
5521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
5581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
5641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
5701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcat
5761 cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc tcgtgacggt
5821 taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt tcaatccgca
5881 cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca
5941 gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc gggttttcga
6001 cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata atttcaccgc
6061 cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa actgttaccc
6121 gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca gcgcggctga
6181 aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca
6241 gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct tccagataac
6301 tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg
6361 cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc cagcgcccgt
6421 tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc tggccttcct
6481 gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg
6541 gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg ggcgcatcgt
6601 aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga agatcgcact
6661 ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat
6721 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
6781 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
6841 cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat tgcagctgct
6901 ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga gcgtgcatgt
6961 ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat caacaggctg
7021 gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg catccaatgc
7081 aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg atcgaagcga
7141 gtgccttcga ctttttcgtg tccagtgtgg ctt
//