LOCUS LT727216 6592 bp DNA circular SYN 06-FEB-2017
DEFINITION Insect expression vector pBlueBacmsGCA1s, complete sequence.
ACCESSION LT727216
VERSION LT727216.1
KEYWORDS .
SOURCE Insect expression vector pBlueBacmsGCA1s
ORGANISM Insect expression vector pBlueBacmsGCA1s
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6592)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6592
/organism="Insect expression vector pBlueBacmsGCA1s"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4469."
/db_xref="taxon:1944739"
regulatory 7..95
/note="AcNPV-PH promoter"
/regulatory_class="promoter"
CDS 214..>1704
/transl_table=11
/note="mGucy1a3 splice variant"
/protein_id="SJL87783.1"
/translation="MFCRKFKDLKITGECPFSLLAPGIIKAAARILYESHVEVSLMPP
CFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPASLFCKTFPFHFMLDRDL
AILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRVRRWD
NSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDV
VLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQ
IVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETI
GDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSPHGEPIKMRIGLHSGSVFAGV
VGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPP
NFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGVD"
misc_feature 1761..1802
/note="V5 epitope"
misc_feature 1812..1829
/note="His-tag"
regulatory 1854..1928
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1854..1928)
/note="SV40 polyA late"
/regulatory_class="terminator"
CDS complement(2116..>2994)
/codon_start=1
/transl_table=11
/note="AcNPV ORF 1629 fragment"
/protein_id="SJL87784.1"
/translation="PETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPP
MVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSS
TTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDVKYVQALFNV
FTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLASFVALSENPL
DSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPKDYKFNELLK
YVQQLSVNQQRTESSA"
CDS 3382..4242
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87785.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 4783..5177
/note="pMB1 ori"
CDS complement(<5178..6290)
/transl_table=11
/note="lacZ fragment"
/protein_id="SJL87786.1"
/translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS
QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP
ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD
SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE
NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
regulatory complement(6291..6565)
/note="AcNPV-ETL promoter"
/regulatory_class="promoter"
BASE COUNT 1638 a 1610 c 1624 g 1720 t
ORIGIN
1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
181 tgcccttagc cactgccaag ctccaggaac accatgttct gcaggaagtt caaggatctc
241 aagatcacag gggagtgtcc tttctcctta ctggcccctg gtatcattaa agcggctgct
301 cgcatactgt acgaaagcca cgtggaggtg tccctgatgc ctccctgctt ccgaagtgac
361 tgtaccgagt ttgtgaacca gccctatttg ctctactccg ttcatgtgaa gagcaccaag
421 ccgtccctgt ccccaggcaa gccccagtcc tcgctagtga tccccgcttc gctcttctgc
481 aagactttcc cgttccattt catgctggac cgagacctgg ccatcctgca gctgggtaac
541 ggcatcagaa ggctggtgaa caagagggac ttccaaggga agcccaactt tgaagagttc
601 tttgaaattc taactcccaa aatcaaccag acatttagtg gcatcatgac aatgttgaac
661 atgcagtttg tcatccgggt gaggagatgg gataactcgg tgaagaaatc gtcaagggtt
721 atggatctca aaggtcaaat gatctacatc gttgaatcca gtgccatctt gttcttaggg
781 tcaccatgtg tggacaggct ggaagatttc acaggacggg ggctctatct gtccgacatc
841 ccaattcata acgccctgag ggatgttgtc ttgatagggg agcaggcacg ggctcaagat
901 ggcctcaaga agaggttggg gaagctgaag gcaaccctgg agcatgccca ccaagccctg
961 gaggaagaga agaagaggac agtggatctg ctgtgctcta tcttcccctc tgaggttgct
1021 cagcagctgt ggcaaggaca aattgtgcaa gccaagaaat tcagcgaggt caccatgctt
1081 ttctcagata tcgtagggtt cactgctatc tgctctcagt gttcacctct gcaggtcatc
1141 acgatgctca acgctctcta cactcgcttt gaccagcagt gtggagagct ggatgtctac
1201 aaggtggaga ccatcgggga tgcatattgt gtggcaggtg gattgcacag agagagtgac
1261 acccatgctg tccagatagc actgatggcc ctgaagatga tggagctctc caatgaggtc
1321 atgtctcccc acggagaacc tatcaagatg cgaattggac tacattctgg atcagtgttt
1381 gctggagttg tcggagtgaa gatgccccgg tattgcctgt ttggaaacaa tgtcactctg
1441 gctaacaaat ttgaatcctg cagtgtgcct cggaaaatca atgtcagccc caccacatac
1501 aggttactca aagactgtcc tggctttgtg ttcaccccga gatcaaggga ggagcttcca
1561 ccaaacttcc ctagtgacat tcctgggatc tgtcactttc tggatgctta tcaccatcaa
1621 ggacctaatt ccaaaccatg gttccaggat aaagatgtgg aagatggaaa cgccaacttc
1681 ttaggcaaag cgtcaggggt agattagtga gccacatgct cttatgtttg atgcctaagg
1741 gcaattcgaa gcttaggcct ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1801 cgcgtaccgg tcatcatcac catcaccatt gacgtctcta gcttggagtc gacaacttgt
1861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
1921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatc
1981 tctcgactct gctgaagagg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa
2041 ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg
2101 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
2161 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
2221 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
2281 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
2341 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
2401 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
2461 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
2521 aaataaagcc tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
2581 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
2641 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
2701 aattatttct gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa
2761 ttcagacaac acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc
2821 tactaatggc ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg
2881 ggctggcggc ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc
2941 aaatgtctct ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgatgg
3001 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
3061 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
3121 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
3181 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
3241 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
3301 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
3361 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
3421 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
3481 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
3541 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
3601 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
3661 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
3721 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
3781 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
3841 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
3901 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
3961 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
4021 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
4081 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
4141 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
4201 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
4261 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
4321 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
4381 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
4441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
4501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
4561 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
4621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
4681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
4741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
4801 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
4861 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
4921 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
4981 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
5041 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
5101 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
5161 tcagtgagcg aggaagcatc ctgcaccatc gtctgctcat ccatgacctg accatgcaga
5221 ggatgatgct cgtgacggtt aacgcctcga atcagcaacg gcttgccgtt cagcagcagc
5281 agaccatttt caatccgcac ctcgcggaaa ccgacatcgc aggcttctgc ttcaatcagc
5341 gtgccgtcgg cggtgtgcag ttcaaccacc gcacgataga gattcgggat ttcggcgctc
5401 cacagtttcg ggttttcgac gttcagacgt agtgtgacgc gatcggcata accaccacgc
5461 tcatcgataa tttcaccgcc gaaaggcgcg gtgccgctgg cgacctgcgt ttcaccctgc
5521 cataaagaaa ctgttacccg taggtagtca cgcaactcgc cgcacatctg aacttcagcc
5581 tccagtacag cgcggctgaa atcatcatta aagcgagtgg caacatggaa atcgctgatt
5641 tgtgtagtcg gtttatgcag caacgagacg tcacggaaaa tgccgctcat ccgccacata
5701 tcctgatctt ccagataact gccgtcactc cagcgcagca ccatcaccgc gaggcggttt
5761 tctccggcgc gtaaaaatgc gctcaggtca aattcagacg gcaaacgact gtcctggccg
5821 taaccgaccc agcgcccgtt gcaccacaga tgaaacgccg agttaacgcc atcaaaaata
5881 attcgcgtct ggccttcctg tagccagctt tcatcaacat taaatgtgag cgagtaacaa
5941 cccgtcggat tctccgtggg aacaaacggc ggattgaccg taatgggata ggtcacgttg
6001 gtgtagatgg gcgcatcgta accgtgcatc tgccagtttg aggggacgac gacagtatcg
6061 gcctcaggaa gatcgcactc cagccagctt tccggcaccg cttctggtgc cggaaaccag
6121 gcaaagcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
6181 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
6241 acgccagggt tttcccagtc acgacgttgt aaaacgacgg gatctatcat ttttagcagt
6301 gattctaatt gcagctgctc tttgatacaa ctaattttac gacgacgatg cgagctttta
6361 ttcaaccgag cgtgcatgtt tgcaatcgtg caagcgttat caatttttca ttatcgtatt
6421 gttgcacatc aacaggctgg acaccacgtt gaactcgccg cagttttgcg gcaagttgga
6481 cccgccgcgc atccaatgca aactttccga cattctgttg cctacgaacg attgattctt
6541 tgtccattga tcgaagcgag tgccttcgac tttttcgtgt ccagtgtggc tt
//