LOCUS LT727215 6910 bp DNA circular SYN 06-FEB-2017
DEFINITION Insect expression vector pBlueBacmsGCB1His, complete sequence.
ACCESSION LT727215
VERSION LT727215.1
KEYWORDS .
SOURCE Insect expression vector pBlueBacmsGCB1His
ORGANISM Insect expression vector pBlueBacmsGCB1His
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6910)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6910
/organism="Insect expression vector pBlueBacmsGCB1His"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4467."
/db_xref="taxon:1944741"
regulatory 7..95
/note="AcNPV-PH promoter"
/regulatory_class="promoter"
CDS 195..>2054
/transl_table=11
/note="msGCB1"
/protein_id="SJL87779.1"
/translation="MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYD
DSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLD
ALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGT
EIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFP
FHIIFDRNLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVF
VLRSKEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLT
RRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDT
LLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLL
NDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAG
QVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSE
YTYRCLMSPENSDPLFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETNEEDEN"
misc_feature 2079..2120
/note="V5 epitope"
misc_feature 2130..2147
/note="His-tag"
regulatory 2172..2246
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2172..2246)
/note="SV40 polyA late"
/regulatory_class="terminator"
CDS complement(2434..>3312)
/codon_start=1
/transl_table=11
/note="3' AcNPV ORF 1629"
/protein_id="SJL87780.1"
/translation="PETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPP
MVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSS
TTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDVKYVQALFNV
FTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLASFVALSENPL
DSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPKDYKFNELLK
YVQQLSVNQQRTESSA"
CDS 3700..4560
/transl_table=11
/note="ampicillin"
/protein_id="SJL87781.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5101..5495
/note="pMB1 ori"
CDS complement(<5496..6608)
/transl_table=11
/note="5' lacZ fragment"
/protein_id="SJL87782.1"
/translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS
QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP
ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD
SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE
NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
regulatory complement(6609..6883)
/note="AcNPV-ETL promoter"
/regulatory_class="promoter"
BASE COUNT 1785 a 1654 c 1688 g 1783 t
ORIGIN
1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
181 tgcccttgga caccatgtac ggtttcgtga accatgccct ggagctgctg gtgatccgca
241 attatggtcc cgaggtgtgg gaagacatca aaaaagaggc acagctggat gaagaaggcc
301 agtttcttgt cagaataata tacgatgatt ccaaaacata tgacctggtg gctgctgcaa
361 gcaaagtcct caacctcaat gctggcgaaa tcctgcagat gtttgggaag atgtttttcg
421 tcttctgcca ggagtctggc tatgatacca tcttgcgtgt cctgggatct aatgtcagag
481 agtttttgca gaacctcgat gccctgcatg accacctcgc caccatttac ccagggatgc
541 gcgcgccttc cttcaggtgc accgatgcgg agaaaggcaa agggctcatc ctgcactact
601 actcggaaag agaggggctt caggacatcg tgatcgggat tatcaagact gttgctcaac
661 agatacacgg cactgagata gacatgaagg tgattcagca aagaaatgaa gaatgtgatc
721 acacccagtt tttaattgaa gaaaaggaat caaaagaaga ggatttttat gaagatctgg
781 ataggtttga agagaatggt acccaggaat cacgtatcag cccttacacc ttctgcaaag
841 catttccttt tcacatcata tttgaccgga acctagtggt cactcagtgt ggcaatgcca
901 tctacagagt gctcccccag ctccagcctg ggaactgcag ccttctgtct gtcttctctc
961 tggtccgccc tcacatcgac atcagtttcc atgggattct ttcacacatc aatacagtct
1021 ttgtactgag aagcaaggaa gggttgctgg atgtggagaa acttgagtgt gaggatgaac
1081 tgactggagc agagattagc tgcttacgtc tcaaaggcca aatgatctac ttaccagaag
1141 cagatagcat cctcttcctc tgttcaccaa gtgtgatgaa cctggacgac ctaacaagaa
1201 gaggcctgta tctgagtgac atccctctcc acgatgctac ccgagacctg gttcttttgg
1261 gagaacagtt ccgggaggag tacaaactga cacaagagct ggaaatcctc accgacaggc
1321 tgcagctcac actgagagcc ttggaggatg agaagaaaaa gacagacaca ttgctgtatt
1381 ctgtccttcc tccatctgtt gccaatgagc tgagacacaa gcgcccagtg cctgccaaaa
1441 gatacgacaa tgtgaccatc ctcttcagcg gcattgtggg cttcaatgct ttctgtagca
1501 agcatgcatc tggagaaggg gccatgaaga ttgtcaatct cctcaacgat ctctacaccc
1561 gatttgacac actgactgat tcacgaaaaa acccatttgt ttacaaggtg gaaacagttg
1621 gtgacaagta tatgacagtg agtggcttgc cagaaccttg tatccaccat gcacggtcca
1681 tttgccacct ggctttagac atgatggaaa ttgctggtca agttcaagta gatggtgaat
1741 ctgttcagat aacaatcggg atccataccg gggaggtggt gacaggtgtg attggacagc
1801 ggatgcctcg gtattgtctc tttgggaata ccgtcaacct cacaagcagg acagaaacca
1861 caggagaaaa gggaaagata aatgtttccg aatatacata caggtgtctc atgtctccag
1921 aaaactcgga tccactgttc catttggagc acagaggccc agtgtctatg aagggcaaga
1981 aggaaccaat gcaagtctgg ttcctatcca ggaaaaatac aggcacggag gaaacaaatg
2041 aggaggatga aaacaagggc aattcgaagc ttaggcctgg taagcctatc cctaaccctc
2101 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga cgtctctagc
2161 ttggagtcga caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
2221 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
2281 tcaatgtatc ttatcatctc tcgactctgc tgaagaggag gaaattctcc ttgaagtttc
2341 cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta
2401 ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg
2461 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
2521 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
2581 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
2641 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
2701 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
2761 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
2821 actcgacgta aacacgttaa ataaagcctg gacatattta acatcgggcg tgttagcttt
2881 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
2941 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
3001 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
3061 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
3121 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
3181 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
3241 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
3301 actggtttcg ggcgatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
3361 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
3421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
3481 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
3541 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
3601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
3661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
3721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
3781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
3841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
3901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
3961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
4021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
4081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
4141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
4201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
4261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
4321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
4381 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
4441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
4501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
4561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
4621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
4681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
4741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
4801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
4861 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
4921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
4981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
5041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
5101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
5161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
5221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
5281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
5341 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
5401 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
5461 acgaccgagc gcagcgagtc agtgagcgag gaagcatcct gcaccatcgt ctgctcatcc
5521 atgacctgac catgcagagg atgatgctcg tgacggttaa cgcctcgaat cagcaacggc
5581 ttgccgttca gcagcagcag accattttca atccgcacct cgcggaaacc gacatcgcag
5641 gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt caaccaccgc acgatagaga
5701 ttcgggattt cggcgctcca cagtttcggg ttttcgacgt tcagacgtag tgtgacgcga
5761 tcggcataac caccacgctc atcgataatt tcaccgccga aaggcgcggt gccgctggcg
5821 acctgcgttt caccctgcca taaagaaact gttacccgta ggtagtcacg caactcgccg
5881 cacatctgaa cttcagcctc cagtacagcg cggctgaaat catcattaaa gcgagtggca
5941 acatggaaat cgctgatttg tgtagtcggt ttatgcagca acgagacgtc acggaaaatg
6001 ccgctcatcc gccacatatc ctgatcttcc agataactgc cgtcactcca gcgcagcacc
6061 atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc tcaggtcaaa ttcagacggc
6121 aaacgactgt cctggccgta accgacccag cgcccgttgc accacagatg aaacgccgag
6181 ttaacgccat caaaaataat tcgcgtctgg ccttcctgta gccagctttc atcaacatta
6241 aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa caaacggcgg attgaccgta
6301 atgggatagg tcacgttggt gtagatgggc gcatcgtaac cgtgcatctg ccagtttgag
6361 gggacgacga cagtatcggc ctcaggaaga tcgcactcca gccagctttc cggcaccgct
6421 tctggtgccg gaaaccaggc aaagcgccat tcgccattca ggctgcgcaa ctgttgggaa
6481 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
6541 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggga
6601 tctatcattt ttagcagtga ttctaattgc agctgctctt tgatacaact aattttacga
6661 cgacgatgcg agcttttatt caaccgagcg tgcatgtttg caatcgtgca agcgttatca
6721 atttttcatt atcgtattgt tgcacatcaa caggctggac accacgttga actcgccgca
6781 gttttgcggc aagttggacc cgccgcgcat ccaatgcaaa ctttccgaca ttctgttgcc
6841 tacgaacgat tgattctttg tccattgatc gaagcgagtg ccttcgactt tttcgtgtcc
6901 agtgtggctt
//