LOCUS LT727213 7766 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pTRKH3, complete sequence.
ACCESSION LT727213
VERSION LT727213.1
KEYWORDS .
SOURCE Vector pTRKH3
ORGANISM Vector pTRKH3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7766)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7766
/organism="Vector pTRKH3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4462."
/db_xref="taxon:1945415"
CDS 543..1280
/transl_table=11
/note="erythromycin resistance"
/protein_id="SJL87771.1"
/translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
KVNNLSTVTYEQVLSIFNSYLLFNGRK"
rep_origin 1935..4850
/note="pAMB1 ori"
CDS 2522..2821
/transl_table=11
/note="repD"
/protein_id="SJL87772.1"
/translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
CDS 2864..4354
/transl_table=11
/note="repE"
/protein_id="SJL87773.1"
/translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
rep_origin complement(5617..6116)
/note="p15A ori"
CDS 6425..7615
/transl_table=11
/note="tetracycline resistance"
/protein_id="SJL87774.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
BASE COUNT 2433 a 1527 c 1683 g 2123 t
ORIGIN
1 gagtctagaa tcgatacgat tttgaagtgg caacagataa aaaaaagcag tttaaaattg
61 ttgctgaact tttaaaacaa gcaaatacaa tcattgtcgc aacagatagc gacagagaag
121 gcgaaaacat tgcctggtcg atcattcata aagcaaatgc cttttctaaa gataaaacgt
181 ataaaagact atggatcaat agtttagaaa aagatgtgat ccgtagcggt tttcaaaatt
241 tgcaaccagg aatgaattac tatccctttt atcaagaagc gcaaaagaaa aacgaaatga
301 tacaccaatc agtgcaaaaa aagatataat gggagataag acggttcgtg ttcgtgctga
361 cttgcaccat atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa aagaagttat
421 ggaaataaga cttagaagca aacttaagag tgtgttgata gtgcagtatc ttaaaatttt
481 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagtgatt
541 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
601 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
661 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
721 acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa
781 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
841 gtattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt
901 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
961 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
1021 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
1081 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
1141 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
1201 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat
1261 tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt
1321 actaaaggga atgtagataa attattaggt atactactga cagcttccaa ggagctaaag
1381 aggtccctag cgctcttatc atggggaagc tcggatcata tgcaagacaa aataaactcg
1441 caacagcact tggagaaatg ggacgaatcg agaaaaccct ctttacgctg gattacatat
1501 ctaataaagc cgtaaggaga cgggttcaaa aaggtttaaa taaaggagaa gcaatcaatg
1561 cattagctag aactatattt tttggacaac gtggagaatt tagagaacgt gctctccaag
1621 accagttaca aagagctagt gcactaaaca taattattaa cgctataagt gtgtggaaca
1681 ctgtatatat ggaaaaagcc gtagaagaat taaaagcaag aggagaattt agagaagatt
1741 taatgccata tgcgtggccg ttaggatggg aacatatcaa ttttcttgga gaatacaaat
1801 ttgaaggatt acatgacact gggcaaatga atttacgtcc tttacgtata aaagagccgt
1861 tttattctta atataacggc tctttttata gaaaaaatcc ttagcgtggt ttttttccga
1921 aatgctggcg gtaccccaag aattagaaat gagtagatca aattattcac gaatagaatc
1981 aggaaaatca gatccaacca taaaaacact agaacaaatt gcaaagttaa ctaactcaac
2041 gctagtagtg gatttaatcc caaatgagcc aacagaacca gagccagaaa cagaatcaga
2101 acaagtaaca ttggatttag aaatggaaga agaaaaaagc aatgacttcg tgtgaataat
2161 gcacgaaatc gttgcttatt tttttttaaa agcggtatac tagatataac gaaacaacga
2221 actgaataga aacgaaaaaa gagccatgac acatttataa aatgtttgac gacattttat
2281 aaatgcatag cccgataaga ttgccaaacc aacgcttatc agttagtcag atgaactctt
2341 ccctcgtaag aagttattta attaactttg tttgaagacg gtatataacc gtactatcat
2401 tatataggga aatcagagag ttttcaagta tctaagctac tgaatttaag aattgttaag
2461 caatcaatcg gaaatcgttt gattgctttt tttgtattca tttatagaag gtggagtttg
2521 tatgaatcat gatgaatgta aaacttatat aaaaaatagt ttattggaga taagaaaatt
2581 agcaaatatc tatacactag aaacgtttaa gaaagagtta gaaaagagaa atatctactt
2641 agaaacaaaa tcagataagt atttttcttc ggagggggaa gattatatat ataagttaat
2701 agaaaataac aaaataattt attcgattag tggaaaaaaa ttgacttata aaggaaaaaa
2761 atctttttca aaacatgcaa tattgaaaca gttgaatgaa aaagcaaacc aagttaatta
2821 aacaacctat tttataggat ttataggaaa ggagaacagc tgaatgaata tcccttttgt
2881 tgtagaaact gtgcttcatg acggcttgtt aaagtacaaa tttaaaaata gtaaaattcg
2941 ctcaatcact accaagccag gtaaaagcaa aggggctatt tttgcgtatc gctcaaaatc
3001 aagcatgatt ggcggtcgtg gtgttgttct gacttccgag gaagcgattc aagaaaatca
3061 agatacattt acacattgga cacccaacgt ttatcgttat ggaacgtatg cagacgaaaa
3121 ccgttcatac acgaaaggac attctgaaaa caatttaaga caaatcaata ccttctttat
3181 tgattttgat attcacacgg caaaagaaac tatttcagca agcgatattt taacaaccgc
3241 tattgattta ggttttatgc ctactatgat tatcaaatct gataaaggtt atcaagcata
3301 ttttgtttta gaaacgccag tctatgtgac ttcaaaatca gaatttaaat ctgtcaaagc
3361 agccaaaata atttcgcaaa atatccgaga atattttgga aagtctttgc cagttgatct
3421 aacgtgtaat cattttggta ttgctcgcat accaagaacg gacaatgtag aattttttga
3481 tcctaattac cgttattctt tcaaagaatg gcaagattgg tctttcaaac aaacagataa
3541 taagggcttt actcgttcaa gtctaacggt tttaagcggt acagaaggca aaaaacaagt
3601 agatgaaccc tggtttaatc tcttattgca cgaaacgaaa ttttcaggag aaaagggttt
3661 aatagggcgt aataacgtca tgtttaccct ctctttagcc tactttagtt caggctattc
3721 aatcgaaacg tgcgaatata atatgtttga gtttaataat cgattagatc aacccttaga
3781 agaaaaagaa gtaatcaaaa ttgttagaag tgcctattca gaaaactatc aaggggctaa
3841 tagggaatac attaccattc tttgcaaagc ttgggtatca agtgatttaa ccagtaaaga
3901 tttatttgtc cgtcaagggt ggtttaaatt caagaaaaaa agaagcgaac gtcaacgtgt
3961 tcatttgtca gaatggaaag aagatttaat ggcttatatt agcgaaaaaa gcgatgtata
4021 caagccttat ttagtgacga ccaaaaaaga gattagagaa gtgctaggca ttcctgaacg
4081 gacattagat aaattgctga aggtactgaa ggcgaatcag gaaattttct ttaagattaa
4141 accaggaaga aatggtggca ttcaacttgc tagtgttaaa tcattgttgc tatcgatcat
4201 taaagtaaaa aaagaagaaa aagaaagcta tataaaggcg ctgacaaatt cttttgactt
4261 agagcataca ttcattcaag agactttaaa caagctagca gaacgcccta aaacggacac
4321 acaactcgat ttgtttagct atgatacagg ctgaaaataa aacccgcact atgccattac
4381 atttatatct atgatacgtg tttgtttttt ctttgctgtt tagcgaatga ttagcagaaa
4441 tatacagagt aagattttaa ttaattatta gggggagaag gagagagtag cccgaaaact
4501 tttagttggc ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg aggggcagtg
4561 agagcgaagc gaacacttga ttttttaatt ttctatcttt tataggtcat tagagtatac
4621 ttatttgtcc tataaactat ttagcagcat aatagattta ttgaataggt catttaagtt
4681 gagcatatta gaggaggaaa atcttggaga aatatttgaa gaacccgatt acatggattg
4741 gattagttct tgtggttacg tggtttttaa ctaaaagtag tgaatttttg atttttggtg
4801 tgtgtgtctt gttgttagta tttgctagtc aaagtgatta aatagaattc cggatgagca
4861 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
4921 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
4981 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
5041 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
5101 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
5161 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
5221 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt
5281 gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
5341 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa
5401 cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc
5461 actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc
5521 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct
5581 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
5641 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
5701 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
5761 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt
5821 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
5881 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
5941 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
6001 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
6061 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
6121 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
6181 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
6241 gatcatctta ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat
6301 gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgttt gacagcttat
6361 catcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
6421 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
6481 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
6541 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
6601 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
6661 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcct
6721 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
6781 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
6841 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
6901 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
6961 cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc
7021 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
7081 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
7141 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
7201 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
7261 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
7321 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
7381 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
7441 gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta
7501 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
7561 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
7621 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
7681 cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
7741 tccagcagcc gcacgcggcg catctc
//