LOCUS LT727212 4541 bp DNA circular SYN 06-FEB-2017 DEFINITION Vector pLT-mCASP-1p30, complete sequence. ACCESSION LT727212 VERSION LT727212.1 KEYWORDS . SOURCE Vector pLT-mCASP-1p30 ORGANISM Vector pLT-mCASP-1p30 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4541) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..4541 /organism="Vector pLT-mCASP-1p30" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4457." /db_xref="taxon:1945332" regulatory 24..40 /note="T7 promoter" /regulatory_class="promoter" misc_feature 41..103 /note="5' UTR of T7 gene 10, incl. RBS" CDS 104..949 /transl_table=11 /note="mCASP-1p30" /protein_id="SJL87769.1" /translation="MGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRTRLAL IICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAACPEH KTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPKVIII QACRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDNVSWR HPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFY LFPGH" misc_feature 950..1061 /note="3' UTR of mCASP-1" regulatory complement(1136..1152) /note="T3 promoter" /regulatory_class="promoter" regulatory 1170..1217 /note="T7 terminator" /regulatory_class="terminator" regulatory 1291..1338 /note="T7 terminator" /regulatory_class="terminator" regulatory 1407..1488 /note="fd terminator" /regulatory_class="terminator" regulatory 1517..1598 /note="fd terminator" /regulatory_class="terminator" rep_origin 1696..2151 /note="f1 ori" rep_origin complement(2155..2528) /note="pMB1 ori" CDS complement(3069..3929) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87770.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4295..4425 /note="PL promoter" /regulatory_class="promoter" BASE COUNT 1244 a 1064 c 1044 g 1189 t ORIGIN 1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 61 ctctagaaat aattttgttt aactttaaga aggagatata catatgggca catttccagg 121 actgactggg accctcaagt tttgcccttt agaaaaagcc cagaagttat ggaaagaaaa 181 tccttcagag atttatccaa taatgaatac aaccactcgt acacgtcttg ccctcattat 241 ctgcaacaca gagtttcaac atctttctcc gagggttgga gctcaagttg acctcagaga 301 aatgaagttg ctgctggagg atctggggta taccgtgaaa gtgaaagaaa atctcacagc 361 tctggagatg gtgaaagagg tgaaagaatt tgctgcctgc ccagagcaca agacttctga 421 cagtactttc cttgtattca tgtctcatgg tatccaggag ggaatatgtg ggaccacata 481 ctctaatgaa gtttcagata ttttaaaggt tgacacaatc tttcagatga tgaacacttt 541 gaagtgccca agcttgaaag acaagcccaa ggtgatcatt attcaggcat gccgtggaga 601 gaaacaagga gtggtgttgt taaaagattc agtaagagac tctgaagagg atttcttaac 661 ggatgcaatt tttgaagatg atggcattaa gaaggcccat atagagaaag attttattgc 721 tttctgctct tcaacaccag ataatgtgtc ttggagacat cctgtcaggg gctcactttt 781 cattgagtca ctcatcaaac acatgaaaga atatgcctgg tcttgtgact tggaggacat 841 tttcagaaag gttcgatttt catttgaaca accagaattt aggctacaga tgcccactgc 901 tgatagggtg accctgacaa aacgtttcta cctcttcccg ggacattaaa cgaagaatcc 961 agttcattct tatgtaccta tgctgagaat cgtgccaata agaagccaat acttccttag 1021 atgatgcaat aaatattaaa ataaaacaaa acagaaaggc taaaaaaaaa aaaaaaaagc 1081 tccgcccgga tccctcgagg tcgacgaatt cgagctcggc cgacttggcc ttccctttag 1141 tgagggttaa taaacttggt gagcaataac tagcataacc ccttggggcc tctaaacggg 1201 tcttgagggg ttttttgctg aaaggaggaa ctatatgcgc tcatacgata tgaacgttga 1261 gactgccgct gagttatcag tgagcaataa ctagcataac cccttggggc ctctaaacgg 1321 gtcttgaggg gttttttgct gaaaggagga actatatccg gatagcttat cgctagaggt 1381 cgaaattcac ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc 1441 tttttttttg gagattttca acgtgaaaaa attattattc gcaattccaa gctaattcac 1501 ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc tttttttttg 1561 gagattttca acgtgaaaaa attattattc gcaattccaa gctctgcctc gcgcgtttcg 1621 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 1681 aagcggatgc agatcacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 1741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 1801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 1861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 1921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 1981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 2041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 2101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tgatctgcgc 2161 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 2221 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 2281 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2341 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2401 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2461 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2521 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2581 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2641 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2701 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 2761 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2881 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 2941 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 3001 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 3061 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 3121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3181 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3361 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 3421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 3601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3661 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 3721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 3901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta tgtaagcaga 3961 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg 4021 agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac 4081 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg 4141 gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg 4201 ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac ctcagcgccg 4261 gtgatgccgg ccacgatgcg tccggcgtag aggatctctc acctaccaaa caatgccccc 4321 ctgcaaaaaa taaattcata taaaaaacat acagataacc atctgcggtg ataaattatc 4381 tctggcggtg ttgacataaa taccactggc ggtgatactg agcacatcag caggacgcac 4441 tgaccaccat gaaggtgacg ctcttaaaat taagccctga agaagggcag cattcaaagc 4501 agaaggcttt ggggtgtgtg atacgaaacg aagcattgga a //