LOCUS       LT727212                4541 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Vector pLT-mCASP-1p30, complete sequence.
ACCESSION   LT727212
VERSION     LT727212.1
KEYWORDS    .
SOURCE      Vector pLT-mCASP-1p30
  ORGANISM  Vector pLT-mCASP-1p30
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Vector pLT-mCASP-1p30"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 4457."
                     /db_xref="taxon:1945332"
     regulatory      24..40
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     misc_feature    41..103
                     /note="5' UTR of T7 gene 10, incl. RBS"
     CDS             104..949
                     /transl_table=11
                     /note="mCASP-1p30"
                     /protein_id="SJL87769.1"
                     /translation="MGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRTRLAL
                     IICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAACPEH
                     KTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPKVIII
                     QACRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDNVSWR
                     HPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFY
                     LFPGH"
     misc_feature    950..1061
                     /note="3' UTR of mCASP-1"
     regulatory      complement(1136..1152)
                     /note="T3 promoter"
                     /regulatory_class="promoter"
     regulatory      1170..1217
                     /note="T7 terminator"
                     /regulatory_class="terminator"
     regulatory      1291..1338
                     /note="T7 terminator"
                     /regulatory_class="terminator"
     regulatory      1407..1488
                     /note="fd terminator"
                     /regulatory_class="terminator"
     regulatory      1517..1598
                     /note="fd terminator"
                     /regulatory_class="terminator"
     rep_origin      1696..2151
                     /note="f1 ori"
     rep_origin      complement(2155..2528)
                     /note="pMB1 ori"
     CDS             complement(3069..3929)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL87770.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4295..4425
                     /note="PL promoter"
                     /regulatory_class="promoter"
BASE COUNT         1244 a         1064 c         1044 g         1189 t
ORIGIN      
        1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
       61 ctctagaaat aattttgttt aactttaaga aggagatata catatgggca catttccagg
      121 actgactggg accctcaagt tttgcccttt agaaaaagcc cagaagttat ggaaagaaaa
      181 tccttcagag atttatccaa taatgaatac aaccactcgt acacgtcttg ccctcattat
      241 ctgcaacaca gagtttcaac atctttctcc gagggttgga gctcaagttg acctcagaga
      301 aatgaagttg ctgctggagg atctggggta taccgtgaaa gtgaaagaaa atctcacagc
      361 tctggagatg gtgaaagagg tgaaagaatt tgctgcctgc ccagagcaca agacttctga
      421 cagtactttc cttgtattca tgtctcatgg tatccaggag ggaatatgtg ggaccacata
      481 ctctaatgaa gtttcagata ttttaaaggt tgacacaatc tttcagatga tgaacacttt
      541 gaagtgccca agcttgaaag acaagcccaa ggtgatcatt attcaggcat gccgtggaga
      601 gaaacaagga gtggtgttgt taaaagattc agtaagagac tctgaagagg atttcttaac
      661 ggatgcaatt tttgaagatg atggcattaa gaaggcccat atagagaaag attttattgc
      721 tttctgctct tcaacaccag ataatgtgtc ttggagacat cctgtcaggg gctcactttt
      781 cattgagtca ctcatcaaac acatgaaaga atatgcctgg tcttgtgact tggaggacat
      841 tttcagaaag gttcgatttt catttgaaca accagaattt aggctacaga tgcccactgc
      901 tgatagggtg accctgacaa aacgtttcta cctcttcccg ggacattaaa cgaagaatcc
      961 agttcattct tatgtaccta tgctgagaat cgtgccaata agaagccaat acttccttag
     1021 atgatgcaat aaatattaaa ataaaacaaa acagaaaggc taaaaaaaaa aaaaaaaagc
     1081 tccgcccgga tccctcgagg tcgacgaatt cgagctcggc cgacttggcc ttccctttag
     1141 tgagggttaa taaacttggt gagcaataac tagcataacc ccttggggcc tctaaacggg
     1201 tcttgagggg ttttttgctg aaaggaggaa ctatatgcgc tcatacgata tgaacgttga
     1261 gactgccgct gagttatcag tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1321 gtcttgaggg gttttttgct gaaaggagga actatatccg gatagcttat cgctagaggt
     1381 cgaaattcac ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc
     1441 tttttttttg gagattttca acgtgaaaaa attattattc gcaattccaa gctaattcac
     1501 ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc tttttttttg
     1561 gagattttca acgtgaaaaa attattattc gcaattccaa gctctgcctc gcgcgtttcg
     1621 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     1681 aagcggatgc agatcacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     1741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     1801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     1861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     1921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     1981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     2041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     2101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tgatctgcgc
     2161 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2221 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2281 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2341 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2401 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2461 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2521 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2581 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2641 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2701 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2761 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2881 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2941 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3001 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3061 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3181 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3361 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     3421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3661 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     3721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta tgtaagcaga
     3961 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
     4021 agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
     4081 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
     4141 gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg
     4201 ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac ctcagcgccg
     4261 gtgatgccgg ccacgatgcg tccggcgtag aggatctctc acctaccaaa caatgccccc
     4321 ctgcaaaaaa taaattcata taaaaaacat acagataacc atctgcggtg ataaattatc
     4381 tctggcggtg ttgacataaa taccactggc ggtgatactg agcacatcag caggacgcac
     4441 tgaccaccat gaaggtgacg ctcttaaaat taagccctga agaagggcag cattcaaagc
     4501 agaaggcttt ggggtgtgtg atacgaaacg aagcattgga a
//