LOCUS LT727211 5683 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pEF-BOS, complete sequence. ACCESSION LT727211 VERSION LT727211.1 KEYWORDS . SOURCE Mammalian expression vector pEF-BOS ORGANISM Mammalian expression vector pEF-BOS other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5683) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5683 /organism="Mammalian expression vector pEF-BOS" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4456." /db_xref="taxon:1945010" rep_origin 207..663 /note="M13 ori" regulatory complement(918..1484) /note="3' UTR of hGM-CSF, incl. polyA" /regulatory_class="terminator" regulatory complement(1948..3091) /note="hEF1 promoter" /regulatory_class="promoter" misc_feature complement(1957..2895) /note="intron hEF1 promoter" CDS complement(<3132..3201) /transl_table=11 /note="part of SV40 small t-antigen" /protein_id="SJL87767.1" /translation="MDKVLNREESLQLMDLLGLERSA" rep_origin complement(3240..3315) /note="SV40 ori" regulatory complement(3272..3443) /note="SV40 early promoter" /regulatory_class="promoter" regulatory complement(3505..3588) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(3680..4082) /note="pMB1 ori" CDS complement(4623..5483) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87768.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1353 a 1558 c 1474 g 1298 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 421 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 541 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa 601 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt 661 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 841 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt 901 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga 961 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc 1021 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc 1081 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc 1141 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga 1201 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa 1261 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc 1321 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc 1381 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac 1441 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag agtcgcggcc 1501 gcgacctgca ggcgcagaac tggtaggtat ggaagatccc tcgagatcca ttgtgctggc 1561 ggtaggcgag cagcgcctgc ctgaagctgc gggattcccg atcagaaatg agcgccagtc 1621 gtcgtcggct ctcggcaccg aagcgtatga ttctccgcca gcatggcttc ggccagtgcg 1681 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg cacccccaac 1741 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa cagtccaggg 1801 ccggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata 1861 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgccagcac aatggatctc 1921 gaggtcgagg gatctctaga gaattcctca cgacacctga aatggaagaa aaaaactttg 1981 aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa 2041 ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg 2101 aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga 2161 gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc 2221 tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg 2281 ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca 2341 cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc 2401 gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg 2461 ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc 2521 gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact 2581 atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt 2641 atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg 2701 cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga 2761 actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt 2821 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc 2881 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc 2941 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac 3001 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac 3061 ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc 3121 ggagcctcac gaggcactcc tttcaagacc tagaaggtcc attagctgca aagattcctc 3181 tctgtttaaa actttatcca tctttgcaaa gctttttgca aaagcctagg cctccaaaaa 3241 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa 3301 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg 3361 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca 3421 tacttctgcc tgctggggag cctaagcttg gcgtaatcat ggtcatagct gtttcctgtg 3481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 3541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 3601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 3661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 3721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 4021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 4081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 4141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 4201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 4261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 4321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 4381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 4441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 4621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 4681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 4741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 4801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 4861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 4921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 4981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 5041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 5101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 5161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 5221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 5281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 5341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 5401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 5461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 5521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 5581 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 5641 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc //