LOCUS LT727210 6245 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-pIL-1B, complete sequence.
ACCESSION LT727210
VERSION LT727210.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-pIL-1B
ORGANISM Mammalian expression vector pCAGGS-pIL-1B
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6245)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6245
/organism="Mammalian expression vector pCAGGS-pIL-1B"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4441."
/db_xref="taxon:1944892"
CDS 26..835
/transl_table=11
/note="hIL1B"
/protein_id="SJL87765.1"
/translation="MAEVPELASEMMAYYSGNEDDLFFEADGPKQMKCSFQDLDLCPL
DGGIQLRISDHHYSKGFRQAASVVVAMDKLRKMLVPCPQTFQENDLSTFFPFIFEEEP
IFFDTWDNEAYVHDAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSM
SFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFN
KIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS"
misc_feature 836..1436
/note="3' UTR of hIL1B"
gap 1437..1453
/estimated_length=17
regulatory 1535..1626
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1627..1983
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2061..2143)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2285..2360
/note="SV40 ori"
regulatory complement(2391..2465)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 2391..2465
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2580..2981)
/note="pMB1 ori"
CDS complement(3522..4382)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87766.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4518..4895
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4901..6242
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5270..6194
/note="intron AG promoter"
BASE COUNT 1398 a 1615 c 1654 g 1561 t
ORIGIN
1 ttcctcgagg aattccgaag cagccatggc agaagtacct gagctcgcca gtgaaatgat
61 ggcttattac agtggcaatg aggatgactt gttctttgaa gctgatggcc ctaaacagat
121 gaagtgctcc ttccaggacc tggacctctg ccctctggat ggcggcatcc agctacgaat
181 ctccgaccac cactacagca agggcttcag gcaggccgcg tcagttgttg tggccatgga
241 caagctgagg aagatgctgg ttccctgccc acagaccttc caggagaatg acctgagcac
301 cttctttccc ttcatctttg aagaagaacc tatcttcttc gacacatggg ataacgaggc
361 ttatgtgcac gatgcacctg tacgatcact gaactgcacg ctccgggact cacagcaaaa
421 aagcttggtg atgtctggtc catatgaact gaaagctctc cacctccagg gacaggatat
481 ggagcaacaa gtggtgttct ccatgtcctt tgtacaagga gaagaaagta atgacaaaat
541 acctgtggcc ttgggcctca aggaaaagaa tctgtacctg tcctgcgtgt tgaaagatga
601 taagcccact ctacagctgg agagtgtaga tcccaaaaat tacccaaaga agaagatgga
661 aaagcgattt gtcttcaaca agatagaaat caataacaag ctggaatttg agtctgccca
721 gttccccaac tggtacatca gcacctctca agcagaaaac atgcccgtct tcctgggagg
781 gaccaaaggc ggccaggata taactgactt caccatgcaa tttgtgtctt cctaaagaga
841 gctgtaccca gagagtcctg tgctgaatgt ggactcaatc cctagggctg gcagaaaggg
901 aacagaaagg tttttgagta cggctatagc ctggactttc ctgttgtcta caccaatgcc
961 caactgcctg ccttagggta gtgctaagag gatctcctgt ccatcagcca ggacagtcag
1021 ctctctcctt tcagggccaa tccccagccc ttttgttgag ccaggcctct ctcacctctc
1081 ctactcactt aaagcccgcc tgacagaaac cacggccaca tttggttcta agaaaccctc
1141 tgtcattcgc tcccacattc tgatgagcaa ccgcttccct atttatttat ttatttgttt
1201 gtttgtttta ttcattggtc taatttattc aaagggggca agaagtagca gtgtctgtaa
1261 aagagcctag tttttaatag ctatggaatc aattcaattt ggactggtgt gctctcttta
1321 aatcaagtcc tttaattaag actgaaaata tataagctca gattatttaa atgggaatat
1381 ttataaatga gcaaatatca tactgttcaa tggttctgaa ataaacttct ctgaagnnnn
1441 nnnnnnnnnn nnngaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
1501 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
1561 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
1621 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
1681 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
1741 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
1801 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
1861 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
1921 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
1981 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
2041 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
2101 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
2161 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
2221 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
2281 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
2341 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
2401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
2461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
2521 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
2581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
2641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag
2701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
2761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
2821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
2881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
2941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
3001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
3061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
3121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
3181 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
3241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
3301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
3361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
3421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
3481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
3541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
3601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
3661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
3721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
3781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
3841 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
3901 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
3961 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
4021 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
4081 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
4141 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
4201 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
4261 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
4321 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
4381 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
4441 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
4501 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
4561 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
4621 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
4681 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
4741 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
4801 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
4861 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
4921 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
4981 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg
5041 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg
5101 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
5161 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc
5221 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
5281 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
5341 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
5401 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
5461 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
5521 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
5581 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
5641 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
5701 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
5761 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg
5821 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
5881 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
5941 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
6001 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
6061 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
6121 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
6181 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
6241 aagaa
//