LOCUS LT727207 5620 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGSpPHGPx, complete sequence.
ACCESSION LT727207
VERSION LT727207.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGSpPHGPx
ORGANISM Mammalian expression vector pCAGGSpPHGPx
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5620)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5620
/organism="Mammalian expression vector pCAGGSpPHGPx"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4431."
/db_xref="taxon:1944893"
misc_feature 83..595
/note="pPHGPx"
misc_feature 596..814
/note="3' UTR of pPHGPx"
regulatory 910..1001
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1002..1358
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1436..1518)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1660..1735
/note="SV40 ori"
regulatory 1766..1840
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1766..1840)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1955..2356)
/note="pMB1 ori"
CDS complement(2897..3757)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87759.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3893..4270
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4276..5617
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4645..5569
/note="intron AG promoter"
BASE COUNT 1205 a 1497 c 1567 g 1351 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gcgggccccc cctcgagaac atatgtgcgc gtcccgcgac gactggcgat gtgctcgctc
121 catgcacgaa ttctcagcca aggacatcga cgggcacatg gtgaacctgg acaagtaccg
181 gggctacgtg tgcatcgtca ccaatgtggc ctctcaatga ggcaagacgg aggtaaacta
241 cactcagctg gtcgacctgc acgcccgata tgctgagtgt ggtttacgga ttctggcctt
301 cccttgcaac cagtttggga ggcaggagcc agggagtgat gctgagatca aagaatttgc
361 tgctggctac aacgtcaaat ttgatatgtt cagcaagatc tgtgtgaatg gggacgatgc
421 ccaccctctg tggaagtgga tgaaagtcca gcccaagggg aggggcatgc tgggaaatgc
481 tatcaaatgg aactttacca agttcctcat tgataagaac ggctgtgtgg tgaagcggta
541 cggtcccatg gaagagcccc aggtcataga gaaggacctg ccgtgctacc tctagctcaa
601 caagtgtgtg ctgagcctgc tgcctgtgcc ctcagagcct tccacctggc acccatgaca
661 gtctgcctaa aaaccagccc tggtggggca gactcgagaa cctggcgtgc aaccccgcca
721 gaggaaggtc ccttggccca gcttgcagct cgcaccccac cccaggctgc cttgtgggaa
781 taaaccatac aaattggcta aaaaaaaaaa aaaaacccga attcctgcag cccgggggat
841 ccactagtaa cggccgccag tgtgctggaa ttctgcagat ccaatgccct ggctcacaaa
901 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
961 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
1021 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
1081 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
1141 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
1201 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
1261 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
1321 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
1381 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
1441 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
1501 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
1561 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
1621 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
1681 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
1741 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
1801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
1861 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
1921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
1981 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
2041 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
2101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
2161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
2221 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
2281 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
2341 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
2401 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
2461 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
2521 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
2581 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
2641 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
2701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
2761 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
2821 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
2881 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
2941 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
3001 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
3061 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
3121 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
3181 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
3241 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
3301 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
3361 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
3421 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
3481 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
3541 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
3601 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
3661 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
3721 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
3781 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
3841 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
3901 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
3961 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
4021 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
4081 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
4141 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
4201 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
4261 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
4321 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
4381 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
4441 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
4501 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
4561 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
4621 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
4681 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
4741 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
4801 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
4861 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
4921 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
4981 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
5041 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
5101 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
5161 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
5221 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
5281 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
5341 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
5401 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
5461 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
5521 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
5581 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//