LOCUS LT727206 5644 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGSbGPx, complete sequence.
ACCESSION LT727206
VERSION LT727206.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGSbGPx
ORGANISM Mammalian expression vector pCAGGSbGPx
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5644)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5644
/organism="Mammalian expression vector pCAGGSbGPx"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4429."
/db_xref="taxon:1944755"
misc_feature 101..718
/note="bGPx"
misc_feature 719..897
/note="3' UTR of bGPx"
regulatory 934..1025
/note="polyA"
/regulatory_class="terminator"
misc_feature 1026..1382
/note="3FR"
regulatory complement(1460..1542)
/note="lac"
/regulatory_class="promoter"
rep_origin 1684..1759
/note="SV40 ori"
regulatory 1790..1864
/note="polyA"
/regulatory_class="terminator"
regulatory complement(1790..1864)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(1979..2380)
/note="pMB1 ori"
CDS complement(2921..3781)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87758.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 3917..4294
/note="hCMV"
regulatory 4300..5641
/note="AG"
/regulatory_class="promoter"
misc_feature 4669..5593
/note="intron"
BASE COUNT 1149 a 1556 c 1578 g 1356 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 ggatccnnng cgctctgctc tggattcgga aacggatacc atgtgcgccg ctcagcgctc
121 ggcggccgcc ctggcggcgg cagccccgcg cacagtgtac gccttctccg cgcgccctct
181 ggccggcggg gagcccttca acctgtcctc cctgcggggc aaggtgctgc tcattgagaa
241 cgtagcatcg ctctgaggca caacggtgcg ggactacacc cagatgaatg acctgcagcg
301 gcgccttgga ccccggggcc tggtcgtgct cggcttcccc tgcaaccagt ttgggcatca
361 ggaaaacgcc aagaacgagg agatcctgaa ttgcctgaag tacgtccgac caggcggcgg
421 gttcgagccc aactttatgc tcttcgaaaa gtgcgaggtg aatggcgaga aggcgcatcc
481 gctcttcgcc ttccttcggg aggttctgcc cacgccaagt gacgacgcca ctgctctcat
541 gaccgaccct aagttcatca cctggtcccc ggtgtgccgc aacgacgtct cctggaactt
601 cgagaagttc ctggtgggcc cagacggtgt gcccgtgcgc aggtacagcc gccgctttct
661 gaccatcgac atcgagcctg acattgaaac cctgctgtcc cagggggcct ctgcctaggg
721 tgcccctccc acccttgctg cttggcggtc agcgctgctc tccagggatt ttgcccatga
781 aggtgttccc tctaaaccta cgtggaggaa tgcctgatgt ccaggaaaat cccctgaggt
841 gggcgctggt cctatccatc ccagcctccc ctttcccaga ccacagctcc tcatgacnng
901 gatcccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
961 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
1021 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
1081 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
1141 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
1201 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
1261 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
1321 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
1381 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
1441 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
1501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
1561 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
1621 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
1681 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
1741 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
1801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
1861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
1921 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
1981 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
2041 tcagctcact caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga
2101 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
2161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
2221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
2281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
2341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
2401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
2461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
2521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
2581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
2641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
2701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
2761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
2821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
2881 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
2941 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
3001 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
3061 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
3121 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
3181 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
3241 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
3301 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
3361 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
3421 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
3481 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
3541 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
3601 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
3661 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
3721 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
3781 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
3841 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
3901 aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg
3961 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
4021 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
4081 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
4141 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
4201 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
4261 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
4321 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
4381 ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg
4441 cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc
4501 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
4561 agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc
4621 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
4681 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
4741 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
4801 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
4861 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
4921 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
4981 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
5041 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
5101 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
5161 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg
5221 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
5281 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
5341 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
5401 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
5461 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
5521 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
5581 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
5641 agaa
//