LOCUS LT727205 9690 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pPP1MFmds1, complete sequence.
ACCESSION LT727205
VERSION LT727205.1
KEYWORDS .
SOURCE Yeast expression vector pPP1MFmds1
ORGANISM Yeast expression vector pPP1MFmds1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9690)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9690
/organism="Yeast expression vector pPP1MFmds1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4426."
/db_xref="taxon:1945495"
regulatory 7..772
/note="AOX1 promoter"
/regulatory_class="promoter"
misc_feature 773..937
/note="5' UTR of AOX1"
CDS 949..>1191
/transl_table=11
/note="ppMF"
/protein_id="SJL87754.1"
/translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDL
EGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVS"
CDS <1228..2727
/codon_start=1
/transl_table=11
/note="mds1 mature sequence"
/protein_id="SJL87755.1"
/translation="ATKRGSPNPTRAAAVKAAFQTSWNAYHHFAFPHDDLHPVSNSFD
DERNGWGSSAIDGLDTAILMGDADIVNTILQYVPQINFTTTAVANQGISVFETNIRYL
GGLLSAYDLLRGPFSSLATNQTLVNSLLRQAQTLANGLKVAFTTPSGVPDPTVFFNPT
VRRSGASSNNVAEIGSLVLEWTRLSDLTGNPQYAQLAQKGESYLLNPKGSPEAWPGLI
GTFVSTSNGTFQDSSGSWSGLMDSFYEYLIKMYLYDPVAFAHYKDRWVLGADSTIGHL
GSHPSTRKDLTFLSSYNGQSTSPNSGHLASFGGGNFILGGILLNEQKYIDFGIKLASS
YFGTYTQTASGIGPEGFAWVDSVTGAGGSPPSSQSGFYSSAGFWVTAPYYILRPETLE
SLYYAYRVTGDSKWQDLAWEALSAIEDACRAGSAYSSINDVTQANGGGASDDMESFWF
AEALKYAYLIFAEESDVQVQATGGNKFVFNTEAHPFSIRSSSRRGGHLA"
regulatory 2988..3247
/note="AOX1 terminator"
/regulatory_class="terminator"
CDS complement(3647..6181)
/transl_table=11
/note="HIS4"
/protein_id="SJL87756.1"
/translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQST
ELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYR
TLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGII
PIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSS
KKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGF
CHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAE
AKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQ
PKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQ
NGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLT
ETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASP
PKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTA
AKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAV
DMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLI
LQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQ
KFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
misc_feature 6536..7293
/note="3' flanking region of AOX1"
rep_origin complement(7577..7979)
/note="pMB1 ori"
CDS complement(8520..9380)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87757.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2487 a 2381 c 2278 g 2544 t
ORIGIN
1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
1201 agagaggctg aagcttacgt agaattcgcc acaaaacgtg gatctcccaa ccctacgagg
1261 gcggcagcag tcaaggccgc attccagacg tcgtggaacg cttaccacca ttttgccttt
1321 ccccatgacg acctccaccc ggtcagcaac agctttgatg atgagagaaa cggctggggc
1381 tcgtcggcaa tcgatggctt ggacacggct atcctcatgg gggatgccga cattgtgaac
1441 acgatccttc agtatgtacc gcagatcaac ttcaccacga ctgcggttgc caaccaaggc
1501 atctccgtgt tcgagaccaa cattcggtac ctcggtggcc tgctttctgc ctatgacctg
1561 ttgcgaggtc ctttcagctc cttggcgaca aaccagaccc tggtaaacag ccttctgagg
1621 caggctcaaa cactggccaa cggcctcaag gttgcgttca ccactcccag cggtgtcccg
1681 gaccctaccg tcttcttcaa ccctactgtc cggagaagtg gtgcatctag caacaacgtc
1741 gctgaaattg gaagcctggt gctcgagtgg acacggttga gcgacctgac gggaaacccg
1801 cagtatgccc agcttgcgca gaagggcgag tcgtatctcc tgaatccaaa gggaagcccg
1861 gaggcatggc ctggcctgat tggaacgttt gtcagcacga gcaacggtac ctttcaggat
1921 agcagcggca gctggtccgg cctcatggac agcttctacg agtacctgat caagatgtac
1981 ctgtacgacc cggttgcgtt tgcacactac aaggatcgct gggtccttgg tgccgactcg
2041 accattgggc atctcggctc tcacccgtcg acgcgcaagg acttgacctt tttgtcttcg
2101 tacaacggac agtctacgtc gccaaactca ggacatttgg ccagttttgg cggtggcaac
2161 ttcatcttgg gaggcattct cctgaacgag caaaagtaca ttgactttgg aatcaagctt
2221 gccagctcgt actttggcac gtacacccag acggcttctg gaatcggccc cgaaggcttc
2281 gcgtgggtgg acagcgtgac gggcgccggc ggctcgccgc cctcgtccca gtccgggttc
2341 tactcgtcgg caggattctg ggtgacggca ccgtattaca tcctgcggcc ggagacgctg
2401 gagagcttgt actacgcata ccgcgtcacg ggcgactcca agtggcagga cctggcgtgg
2461 gaagcgttga gtgccattga ggacgcatgc cgcgccggca gcgcgtactc gtccatcaac
2521 gacgtgacgc aggccaacgg cgggggtgcc tctgacgata tggagagctt ctggtttgcc
2581 gaggcgctca agtatgcgta cctgatcttt gcggaggagt cggatgtgca ggtgcaggcc
2641 accggcggga acaaatttgt ctttaacacg gaggcgcacc cctttagcat ccgttcatca
2701 tcacgacggg gcggccacct tgcttaaagg cgctgagagt tctctcgcct gtttttttgc
2761 tcgtattatt cattttctgc tgctcgtccc gaatcgctcc gtagatattt tgacacatgc
2821 gtctggatag agcggctggg accaccgaat atggtatgtc attcgagata tgaagcgcaa
2881 gagatccggg ccctctagat gcggccgcga attaattcgc cttagacatg actgttcctc
2941 agttcaagtt gggcacttac gagaagaccg gtcttgctag attctaatca agaggatgtc
3001 agaatgccat ttgcctgaga gatgcaggct tcatttttga tactttttta tttgtaacct
3061 atatagtata ggattttttt tgtcattttg tttcttctcg tacgagcttg ctcctgatca
3121 gcctatctcg cagctgatga atatcttgtg gtaggggttt gggaaaatca ttcgagtttg
3181 atgtttttct tggtatttcc cactcctctt cagagtacag aagattaagt gagaagttcg
3241 tttgtgcaag cttatcgata agctttaatg cggtagttta tcacagttaa attgctaacg
3301 cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
3361 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat
3421 cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat
3481 gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt
3541 cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt
3601 cctgtggatc tatcgaatct aaatgtaagt taaaatctct aaataattaa ataagtccca
3661 gtttctccat acgaacctta acagcattgc ggtgagcatc tagaccttca acagcagcca
3721 gatccatcac tgcttggcca atatgtttca gtccctcagg agttacgtct tgtgaagtga
3781 tgaacttctg gaaggttgca gtgttaactc cgctgtattg acgggcatat ccgtacgttg
3841 gcaaagtgtg gttggtaccg gaggagtaat ctccacaact ctctggagag taggcaccaa
3901 caaacacaga tccagcgtgt tgtacttgat caacataaga agaagcattc tcgatttgca
3961 ggatcaagtg ttcaggagcg tactgattgg acatttccaa agcctgctcg taggttgcaa
4021 ccgatagggt tgtagagtgt gcaatacact tgcgtacaat ttcaaccctt ggcaactgca
4081 cagcttggtt gtgaacagca tcttcaattc tggcaagctc cttgtctgtc atatcgacag
4141 ccaacagaat cacctgggaa tcaataccat gttcagcttg agacagaagg tctgaggcaa
4201 cgaaatctgg atcagcgtat ttatcagcaa taactagaac ttcagaaggc ccagcaggca
4261 tgtcaatact acacagggct gatgtgtcat tttgaaccat catcttggca gcagtaacga
4321 actggtttcc tggaccaaat attttgtcac acttaggaac agtttctgtt ccgtaagcca
4381 tagcagctac tgcctgggcg cctcctgcta gcacgataca cttagcacca accttgtggg
4441 caacgtagat gacttctggg gtaagggtac catccttctt aggtggagat gcaaaaacaa
4501 tttctttgca accagcaact ttggcaggaa cacccagcat cagggaagtg gaaggcagaa
4561 ttgcggttcc accaggaata tagaggccaa ctttctcaat aggtcttgca aaacgagagc
4621 agactacacc agggcaagtc tcaacttgca acgtctccgt tagttgagct tcatggaatt
4681 tcctgacgtt atctatagag agatcaatgg ctctcttaac gttatctggc aattgcataa
4741 gttcctctgg gaaaggagct tctaacacag gtgtcttcaa agcgactcca tcaaacttgg
4801 cagttagttc taaaagggct ttgtcaccat tttgacgaac attgtcgaca attggtttga
4861 ctaattccat aatctgttcc gttttctgga taggacgacg aagggcatct tcaatttctt
4921 gtgaggaggc cttagaaacg tcaattttgc acaattcaat acgaccttca gaagggactt
4981 ctttaggttt ggattcttct ttaggttgtt ccttggtgta tcctggcttg gcatctcctt
5041 tccttctagt gacctttagg gacttcatat ccaggtttct ctccacctcg tccaacgtca
5101 caccgtactt ggcacatcta actaatgcaa aataaaataa gtcagcacat tcccaggcta
5161 tatcttcctt ggatttagct tctgcaagtt catcagcttc ctccctaatt ttagcgttca
5221 acaaaacttc gtcgtcaaat aaccgtttgg tataagaacc ttctggagca ttgctcttac
5281 gatcccacaa ggtggcttcc atggctctaa gaccctttga ttggccaaaa caggaagtgc
5341 gttccaagtg acagaaacca acacctgttt gttcaaccac aaatttcaag cagtctccat
5401 cacaatccaa ttcgataccc agcaactttt gagttgctcc agatgtagca cctttatacc
5461 acaaaccgtg acgacgagat tggtagactc cagtttgtgt ccttatagcc tccggaatag
5521 actttttgga cgagtacacc aggcccaacg agtaattaga agagtcagcc accaaagtag
5581 tgaatagacc atcggggcgg tcagtagtca aagacgccaa caaaatttca ctgacaggga
5641 actttttgac atcttcagaa agttcgtatt cagtagtcaa ttgccgagca tcaataatgg
5701 ggattatacc agaagcaaca gtggaagtca catctaccaa ctttgcggtc tcagaaaaag
5761 cataaacagt tctactaccg ccattagtga aacttttcaa atcgcccagt ggagaagaaa
5821 aaggcacagc gatactagca ttagcgggca aggatgcaac tttatcaacc agggtcctat
5881 agataaccct agcgcctggg atcatccttt ggacaactct ttctgccaaa tctaggtcca
5941 aaatcacttc attgatacca ttattgtaca acttgagcaa gttgtcgatc agctcctcaa
6001 attggtcctc tgtaacggat gactcaactt gcacattaac ttgaagctca gtcgattgag
6061 tgaacttgat caggttgtgc agctggtcag cagcataggg aaacacggct tttcctacca
6121 aactcaagga attatcaaac tctgcaacac ttgcgtatgc aggtagcaag ggaaatgtca
6181 tacttgaagt cggacagtga gtgtagtctt gagaaattct gaagccgtat ttttattatc
6241 agtgagtcag tcatcaggag atcctctacg ccggacgcat cgtggccggc atcaccggcg
6301 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
6361 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
6421 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
6481 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
6541 tatctatgat tggaagtatg ggaatggtga tacccgcatt cttcagtgtc ttgaggtctc
6601 ctatcagatt atgcccaact aaagcaaccg gaggaggaga tttcatggta aatttctctg
6661 acttttggtc atcagtagac tcgaactgtg agactatctc ggttatgaca gcagaaatgt
6721 ccttcttgga gacagtaaat gaagtcccac caataaagaa atccttgtta tcaggaacaa
6781 acttcttgtt tcgaactttt tcggtgcctt gaactataaa atgtagagtg gatatgtcgg
6841 gtaggaatgg agcgggcaaa tgcttacctt ctggaccttc aagaggtatg tagggtttgt
6901 agatactgat gccaacttca gtgacaacgt tgctatttcg ttcaaaccat tccgaatcca
6961 gagaaatcaa agttgtttgt ctactattga tccaagccag tgcggtcttg aaactgacaa
7021 tagtgtgctc gtgttttgag gtcatctttg tatgaataaa tctagtcttt gatctaaata
7081 atcttgacga gccaaggcga taaataccca aatctaaaac tcttttaaaa cgttaaaagg
7141 acaagtatgt ctgcctgtat taaaccccaa atcagctcgt agtctgatcc tcatcaactt
7201 gaggggcact atcttgtttt agagaaattt gcggagatgc gatatcgaga aaaaggtacg
7261 ctgattttaa acgtgaaatt tatctcaaga tctctgcctc gcgcgtttcg gtgatgacgg
7321 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
7381 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
7441 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
7501 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
7561 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
7621 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
7681 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
7741 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
7801 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
7861 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
7921 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
7981 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
8041 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
8101 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
8161 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
8221 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
8281 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
8341 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
8401 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
8461 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
8521 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
8581 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
8641 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
8701 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
8761 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
8821 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
8881 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
8941 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
9001 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
9061 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
9121 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
9181 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
9241 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
9301 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
9361 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
9421 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
9481 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
9541 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattctcatg
9601 tttgacagct tatcatcgat aagctgactc atgttggtat tgtgaaatag acgcagatcg
9661 ggaacactga aaaataacag ttattattcg
//