LOCUS LT727204 5475 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pPICZTS, complete sequence.
ACCESSION LT727204
VERSION LT727204.1
KEYWORDS .
SOURCE Yeast expression vector pPICZTS
ORGANISM Yeast expression vector pPICZTS
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5475)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5475
/organism="Yeast expression vector pPICZTS"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4420."
/db_xref="taxon:1945492"
regulatory 7..772
/note="AOX1 promoter"
/regulatory_class="promoter"
misc_feature 773..937
/note="5' UTR of AOX1"
CDS 949..>1191
/transl_table=11
/note="ppMF"
/protein_id="SJL87751.1"
/translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDL
EGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVS"
CDS 1219..>3150
/transl_table=11
/note="mature TS"
/protein_id="SJL87752.1"
/translation="MLAPGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNV
DGVMVAIADARYDTSNDNSLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVI
VKGNKLYVLVGSYYSSRSYWSSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVS
LKKFFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKT
WKFGKGRSDFGCSEPVALEWEGKLIINTRVDWKRRLVYESSDMGNTWVEAVGTLSRVW
GPSPKSDHPGSQSSFTAVTIEGMRVMLFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQ
VSIGDENSAYSSVLYKDDKLYCLHEINTDEVYSLVFARLVGELRIIKSVLRSWKNWDS
HLSSICTPADPAASSSESGCGPAVTTVGLVGFLSGNASQNVWEDAYRCVNASTANAER
VRNGLKFAGVGGGALWPVSQQGQNQRYRFANHAFTLVASVTIHEAPRAASPLLGASLD
SSGGKKLLGLSYDEKHQWQPIYGSTPVTPTGSWETGKRYHVVLTVANKIGSVYIDGEL
LEGSGQTVVPDGRTPDISHFYVGGYGRSDMPTISHVTVNNVLLYNRQLNTEEIRTLFL
SQDLIGTEAHMDSSSDTKEL"
misc_feature 3157..3186
/note="Myc-tag"
misc_feature 3202..3219
/note="His-tag"
regulatory 3299..3558
/note="AOX1 terminator"
/regulatory_class="terminator"
regulatory 3565..3970
/note="TEF1 promoter"
/regulatory_class="promoter"
regulatory 3979..4044
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 4045..4419
/transl_table=11
/note="zeocin resistance"
/protein_id="SJL87753.1"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
GREFALRDPAGNCVHFVAEEQD"
regulatory 4465..4735
/note="CYC1 terminator"
/regulatory_class="terminator"
rep_origin complement(4745..5025)
/note="pMB1 ori"
BASE COUNT 1324 a 1327 c 1387 g 1437 t
ORIGIN
1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
1201 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt
1261 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac
1321 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct
1381 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac
1441 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct
1501 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc
1561 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg
1621 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa
1681 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca
1741 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac
1801 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat
1861 gatgggaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg
1921 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg
1981 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt
2041 gtgtggggcc cctcaccaaa atcggaccat cccggcagtc agagcagctt cactgccgtg
2101 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg
2161 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa
2221 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg
2281 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt
2341 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg
2401 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc
2461 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg
2521 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt
2581 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat
2641 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag
2701 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa
2761 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg
2821 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg
2881 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt
2941 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt
3001 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg
3061 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac
3121 atggacagca gcagcgacac gaaggaattg ggtctagaac aaaaactcat ctcagaagag
3181 gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttgtag ccttagacat
3241 gactgttcct cagttcaagt tgggcactta cgagaagacc ggtcttgcta gattctaatc
3301 aagaggatgt cagaatgcca tttgcctgag agatgcaggc ttcatttttg atactttttt
3361 atttgtaacc tatatagtat aggatttttt ttgtcatttt gtttcttctc gtacgagctt
3421 gctcctgatc agcctatctc gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc
3481 attcgagttt gatgtttttc ttggtatttc ccactcctct tcagagtaca gaagattaag
3541 tgagaccttc gtttgtgcgg atcccccaca caccatagct tcaaaatgtt tctactcctt
3601 ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa
3661 gcacagcata ctaaattttc cctctttctt cctctagggt gtcgttaatt acccgtacta
3721 aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat
3781 aaaaattttt atcacgtttc tttttcttga aatttttttt tttagttttt ttctctttca
3841 gtgacctcca ttgatattta agttaataaa cggtcttcaa tttctcaagt ttcagtttca
3901 tttttcttgt tctattacaa ctttttttac ttcttgttca ttagaaagaa agcatagcaa
3961 tctaatctaa ggggcggtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
4021 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
4081 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
4141 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
4201 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
4261 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
4321 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
4381 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtccgacg gcggcccacg
4441 ggtcccaggc ctcggagatc cgtccccctt ttcctttgtc gatatcatgt aattagttat
4501 gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag
4561 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt
4621 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat
4681 actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcaagctgga
4741 gaccaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
4801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
4861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
4921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
4981 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
5041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
5101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
5161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
5221 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
5281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
5341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
5401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
5461 tttggtcatg agatc
//