LOCUS LT727200 6557 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-E-hABIN-2, complete sequence. ACCESSION LT727200 VERSION LT727200.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-E-hABIN-2 ORGANISM Mammalian expression vector pCAGGS-E-hABIN-2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6557) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6557 /organism="Mammalian expression vector pCAGGS-E-hABIN-2" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4368." /db_xref="taxon:1944771" misc_feature 13..51 /note="E-tag" CDS 64..1353 /transl_table=11 /note="hABIN-2" /protein_id="SJL87745.1" /translation="MSRDPGSGGWEEAPRAAAALCTLYHEAGQRLRRLQDQLAARDAL IARLRARLAALEGDAAPSLVDALLEQVARFREQLRRQEGGAAEAQMRQEIERLTERLE EKEREMQQLLSQPQHEREKEVVLLRRSMAEGERARAASDVLCRSLANETHHLRRTLTA TAHMCQHLAKCLDERQHAQRNVGERSPDQSEHTDGHTSVQSVIEKLQEENRLLKQKVT HVEDLNAKWQRYNASRDEYVRGLHAQLRGLQIPHEPELMRKEISRLNRQLEEKINDCA EVKQELAASRTARDAALERVQMLEQQILAYKDDFMSERADRERAQSRIQELEEKVASL LHQVSWRQDSREPDAGRIHAGSKTAKYLAADALELMVPGGWRPGTGSQQPEPPAEGGH PGAAQRGQGDLQCPHCLQCFSDEQGEELLRHVAECCQ" regulatory 1847..1938 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 1939..2295 /note="3' FR of beta-globin" regulatory complement(2373..2455) /note="lac promoter" /regulatory_class="promoter" rep_origin 2597..2672 /note="SV40 ori" regulatory 2703..2777 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2703..2777) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(2892..3293) /note="pMB1 ori" CDS complement(3834..4694) /transl_table=11 /note="ampicillin" /protein_id="SJL87746.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4830..5207 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 5213..6554 /note="AG promoter" /regulatory_class="promoter" misc_feature 5582..6506 /note="intron AG promoter" BASE COUNT 1370 a 1775 c 1938 g 1474 t ORIGIN 1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggt 61 accatgtccc gggacccggg gtcgggcggc tgggaggagg ccccgcgcgc agctgccgcg 121 ctctgcaccc tgtaccacga ggccggacag cggctgcgcc gcctgcagga ccagctcgct 181 gcccgcgacg ccctcatcgc tcgcctccgc gcccgcctgg ccgcgctgga gggggacgcc 241 gcgccgtccc tagtggacgc gctgctggag caggttgcgc gcttccggga gcagctgcga 301 aggcaggagg gcggcgccgc cgaggcccag atgcgccagg aaattgagag gctgactgag 361 cgactagaag aaaaagagag ggagatgcag cagctgctga gccagcccca acacgagcga 421 gagaaggaag tcgtcctgct acggaggagc atggcagaag gggagcgcgc ccgggccgcc 481 agtgacgtcc tgtgccgctc cttggccaac gagacccatc atctgcggag gacgctgacc 541 gccaccgccc acatgtgtca gcatctggcc aagtgtctgg atgaacgaca gcatgcacaa 601 aggaatgtgg gggagagaag tcctgaccag tcggaacaca cagatgggca cacctctgtc 661 cagagtgtta ttgagaagtt gcaggaagaa aatcgactgt taaaacagaa ggtgactcac 721 gttgaagacc tcaatgccaa gtggcagcgc tacaacgcca gcagggacga atacgtgagg 781 gggctccatg cgcagctcag ggggctgcag atcccccacg agcccgagct gatgaggaag 841 gagatctccc ggctcaacag acagttggaa gagaaaataa atgactgtgc cgaagtgaag 901 caggagctgg cggcctccag gacggcccgg gatgctgcgt tggagcgggt gcagatgctg 961 gaacagcaga ttctcgctta caaggatgac ttcatgtcag aaagggccga tcgggaacgg 1021 gctcaaagta ggattcaaga actggaggaa aaggtcgcct ctttgctgca ccaggtgtcc 1081 tggagacagg attctcgaga gccagacgcc ggccggattc acgctgggag caaaactgcc 1141 aagtatttgg ccgccgacgc attagagctt atggtgcctg gtggctggag gcctgggact 1201 gggtcccagc agccagaacc ccctgcagag ggcgggcatc ctggcgcggc ccagagaggc 1261 cagggggacc ttcagtgccc tcactgcctg cagtgcttca gtgacgagca aggggaagag 1321 ctcctcaggc atgtggccga gtgctgccag tgaccgagac tcacccgtgc ccttgcggcc 1381 tcctggcccg gtgcagctgc cctcagggac agggtgggtg ctctcagatg ccatgggttg 1441 agctctactg agagccaagg cccctagaat agttgcgggg cactctgatc gttcactttg 1501 gtccctttgg ctatggaaca ggctgggtca cagggaactg ccagtgaggc tggaggctgg 1561 aggtggagat ggggtcagga acatctggca gagggaggtc ccagtctgtg tctccatcag 1621 gcttaagcca gagctatctg gtgctggtgt gccagcccct cccccagcct gcctagaaag 1681 gggtggctgc ctgagggagt cacttgtatg gtccccaggg tgggagcccc atcctgttct 1741 atggaataaa gcgtcgcctc tctgcctcga accagtcaaa tggagtattg cggctgcacg 1801 tcacactagg gtggccaccc ccgccattgc gagccacatg tctgcggatc tttttccctc 1861 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag 1921 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac 1981 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac 2041 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat 2101 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat 2161 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca 2221 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct 2281 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt 2341 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 2401 agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 2461 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca 2521 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 2581 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 2641 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 2701 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 2761 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 2821 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 2881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 2941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat 3001 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 3061 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 3121 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 3181 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 3241 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 3301 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 3361 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 3421 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 3481 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 3541 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 3601 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 3661 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 3721 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 3781 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 3841 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 3901 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 3961 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 4021 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 4081 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 4141 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 4201 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 4261 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 4321 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 4381 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 4441 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 4501 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 4561 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 4621 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 4681 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 4741 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 4801 acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta 4861 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 4921 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 4981 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 5041 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 5101 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 5161 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag 5221 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt 5281 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc 5341 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc 5401 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc 5461 ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc 5521 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca 5581 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg 5641 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg 5701 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg 5761 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg 5821 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga 5881 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc 5941 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc 6001 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg 6061 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga 6121 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt 6181 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg 6241 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg 6301 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc 6361 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg 6421 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg 6481 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc 6541 atcattttgg caaagaa //