LOCUS LT727199 5752 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hABIN-3, complete sequence.
ACCESSION LT727199
VERSION LT727199.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hABIN-3
ORGANISM Mammalian expression vector pCAGGS-E-hABIN-3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5752)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5752
/organism="Mammalian expression vector pCAGGS-E-hABIN-3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4367."
/db_xref="taxon:1944772"
misc_feature 16..54
/note="E-tag"
CDS 64..1023
/transl_table=11
/note="hABIN-3"
/protein_id="SJL87743.1"
/translation="MAHFVQGTSRMIAAESSTEHKECAEPSTRKNLMNSLEQKIRCLE
KQRKELLEVNQQWDQQFRSMKELYERKVAELKTKLDAAERFLSTREKDPHQRQRKDDR
QREDDRQRDLTRDRLQREEKEKERLNEELHELKEENKLLKGKNTLANKEKEHYECEIK
RLNKALQDALNIKCSFSEDCLRKSRVEFCHEEMRTEMEVLKQQVQIYEEDFKKERSDR
ERLNQEKEELQQINETSQSQLNRLNSQIKACQMEKEKLEKQLKQMYCPPCNCGWFSTC
KIHGYQQALELCRSNGSTHQTISGMLLTSFRQMYNTRQMVYPQ"
regulatory 1042..1133
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1134..1490
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1568..1650)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1792..1867
/note="SV40 ori"
regulatory 1898..1972
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1898..1972)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2087..2488)
/note="pMB1 ori"
CDS complement(3029..3889)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87744.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4025..4402
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4408..5749
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4777..5701
/note="intron AG promoter"
BASE COUNT 1354 a 1453 c 1583 g 1362 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctatggcac attttgtaca aggcacatct agaatgattg ccgcagaaag ttctacggag
121 cataaagagt gtgctgaacc atcaacaaga aagaacttga tgaattctct tgaacaaaag
181 ataaggtgtt tggaaaaaca aagaaaagag ctcctggaag ttaaccagca atgggatcag
241 caatttagaa gtatgaaaga gttatatgaa agaaaggtag cagagctgaa gacgaaactg
301 gacgccgcgg aaagattcct cagcacgcgg gagaaggatc cgcatcagag gcagagaaag
361 gacgacaggc agagagagga cgacaggcag cgcgacctga cccgggaccg gctgcagcgg
421 gaggagaagg aaaaggaacg cctaaatgaa gaattacatg aattgaaaga agagaataaa
481 cttttaaagg gaaaaaatac tcttgcgaac aaggaaaagg aacattacga atgtgaaata
541 aaacgcctca ataaggctct tcaggatgcc ttgaatatca agtgttcatt ttccgaggac
601 tgtttgagga agtctcgagt ggaattctgc catgaggaga tgagaacaga aatggaagtt
661 ctgaagcagc aggtgcaaat atacgaagaa gacttcaaaa aggaacgatc ggatcgagag
721 agacttaatc aagagaaaga ggagctacag caaattaatg aaacttccca atcccagttg
781 aacaggctga attcccagat aaaagcttgt cagatggaga aagaaaaact agaaaagcaa
841 ttaaaacaga tgtattgccc accctgtaac tgcggctggt tttccacctg caagatccat
901 gggtaccaac aggccctgga gctgtgcaga agcaacggga gcacccacca gactatcagt
961 ggtatgctct tgaccagctt ccgccagatg tacaacacaa ggcaaatggt ttatcctcag
1021 taaagaaagt ccatccgtag agatcttttt ccctctgcca aaaattatgg ggacatcatg
1081 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
1141 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
1201 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
1261 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
1321 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
1381 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
1441 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
1501 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
1561 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga
1621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
1681 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
1741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
1801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
1861 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
1921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
1981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
2041 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
2101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
2161 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa
2221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
2281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
2341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
2401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
2461 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
2521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
2581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
2641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
2701 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
2761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
2821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
2881 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
2941 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
3001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
3061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
3121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
3181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
3241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
3301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
3361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
3421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
3481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
3541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
3601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
3661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
3721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
3781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
3841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
3901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
3961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
4021 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
4081 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
4141 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
4201 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
4261 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
4321 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
4381 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
4441 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
4501 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
4561 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
4621 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
4681 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
4741 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
4801 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
4861 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
4921 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
4981 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
5041 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
5101 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
5161 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
5221 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
5281 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
5341 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
5401 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
5461 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
5521 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
5581 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
5641 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
5701 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
//