LOCUS LT727196 4307 bp DNA circular SYN 06-FEB-2017
DEFINITION Plant expression vector pAS104, complete sequence.
ACCESSION LT727196
VERSION LT727196.1
KEYWORDS .
SOURCE Plant expression vector pAS104
ORGANISM Plant expression vector pAS104
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4307)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4307
/organism="Plant expression vector pAS104"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4356."
/db_xref="taxon:1945179"
regulatory 95..178
/note="lac"
/regulatory_class="promoter"
regulatory 239..255
/note="SP6"
/regulatory_class="promoter"
regulatory complement(336..694)
/note="E35S"
/regulatory_class="promoter"
regulatory complement(766..782)
/note="T7"
/regulatory_class="promoter"
rep_origin 947..1360
/note="f1 ori"
CDS 1695..2489
/transl_table=11
/note="neomycin resistance"
/protein_id="SJL87737.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSRLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVIHGDACLPNIMVENGRFSGFNDCGRLGVADRY
QDIALDTRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2507..3367
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87738.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 3908..4307
/note="pMB1 ori"
BASE COUNT 1037 a 1090 c 1138 g 1042 t
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
301 ctagtaacgg ccgccagtgt gctggaattc ggcttccttc aatttaaccg atgctaatga
361 gttatttttg catgctttaa tttgtttcta tcaaatgttt attttttttt actagaaata
421 acttattgca tttcattcaa aataagatca tacatacagg ttaaaataaa catagggaac
481 ccaaatggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
541 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcac
601 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
661 actgacgtaa gggatgacgc acaatcccac tatcaagccg aattctgcag atatccatca
721 cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat
781 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
841 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
901 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
1021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
1081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagagc tttacggcac
1141 ctcgaccgca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
1201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
1261 actggaacaa cactcaaccc tatcgcggtc tattcttttg atttataagg gattttgccg
1321 atttcggcct attggttaaa aaatgagctg atttaacaaa ttcagggcgc aagggctgct
1381 aaaggaaccg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
1441 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
1501 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
1561 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
1621 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
1681 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
1741 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
1801 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
1861 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
1921 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
1981 aagtgccggg gcaggatctc ctgtcatctc gccttgctcc tgccgagaaa gtatccatca
2041 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
2101 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
2161 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
2221 cgcgcatgcc cgacggcgag gatctcgtcg tgatccatgg cgatgcctgc ttgccgaata
2281 tcatggtgga aaatggccgc ttttctggat tcaacgactg tggccggctg ggtgtggcgg
2341 accgctatca ggacatagcg ttggataccc gtgatattgc tgaagagctt ggcggcgaat
2401 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
2461 tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga gtattcaaca
2521 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
2581 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
2641 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
2701 aatgatgagc acttttaaag ttctgctatg tgatacacta ttatcccgta ttgacgccgg
2761 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
2821 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
2881 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
2941 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
3001 ggagctgaat gaagccatac caaacgacga gagtgacacc acgatgcctg tagcaatgcc
3061 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
3121 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
3181 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
3241 agcactgggg ccagatggta agcgctcccg tatcgtagtt atctacacga cggggagtca
3301 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
3361 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
3421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
3481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
3541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
3601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
3661 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
3721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
3781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
3841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
3901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
3961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
4021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
4081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
4141 ggccttttta cggttcctgg gcttttgctg gccttttgct cacatgttct ttcctgcgtt
4201 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
4261 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//