LOCUS LT727195 5058 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-1p10HA, complete sequence.
ACCESSION LT727195
VERSION LT727195.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-1p10HA
ORGANISM Mammalian expression vector pCAGGS-mCASP-1p10HA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5058)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5058
/organism="Mammalian expression vector
pCAGGS-mCASP-1p10HA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4347."
/db_xref="taxon:1944880"
CDS 10..>276
/transl_table=11
/note="mCASP-1, p10 subunit"
/protein_id="SJL87735.1"
/translation="MGIKKAHIEKDFIAFCSSTPDNVSWRHPVRGSLFIESLIKHMKE
YAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFYLFPGH"
misc_feature 286..312
/note="HA epitope"
regulatory 348..439
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 440..796
/note="3' FR of beta-globin"
regulatory complement(874..956)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1098..1173
/note="SV40 ori"
regulatory 1204..1278
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1204..1278)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1393..1794)
/note="pMB1 ori"
CDS complement(2335..3195)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87736.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3331..3708
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3714..5055
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4083..5007
/note="intron AG promoter"
BASE COUNT 1079 a 1332 c 1383 g 1264 t
ORIGIN
1 ttcctcgaga tgggcattaa gaaggcccat atagagaaag attttattgc tttctgctct
61 tcaacaccag ataatgtgtc ttggagacat cctgtcaggg gctcactttt cattgagtca
121 ctcatcaaac acatgaaaga atatgcctgg tcttgtgact tggaggacat tttcagaaag
181 gttcgatttt catttgaaca accagaattt aggctacaga tgcccactgc tgatagggtg
241 accctgacaa aacgtttcta cctcttcccc ggacatagcg gccgctaccc atacgatgtt
301 ccagattacg cttaatggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
361 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
421 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
481 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
541 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
601 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
661 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
721 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
781 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
901 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
961 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
1021 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
1081 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
1141 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
1201 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
1261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
1321 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
1381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
1441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga
1501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
1561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
1621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
1681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
1741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
1801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
1861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
1921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
1981 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
2041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
2101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
2161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
2221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
2281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
2341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
2401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
2461 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
2521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
2581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
2641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
2701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
2761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
2821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
2881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
2941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
3001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
3061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
3121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
3181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
3241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
3301 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
3361 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
3421 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
3481 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
3541 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
3601 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
3661 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
3721 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
3781 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc
3841 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag
3901 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
3961 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc
4021 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
4081 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
4141 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
4201 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
4261 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
4321 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
4381 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
4441 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
4501 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
4561 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
4621 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
4681 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
4741 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
4801 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
4861 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
4921 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
4981 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
5041 catcattttg gcaaagaa
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