LOCUS LT727194 9021 bp DNA circular SYN 06-FEB-2017
DEFINITION Shuttle vector pRM1, complete sequence.
ACCESSION LT727194
VERSION LT727194.1
KEYWORDS .
SOURCE Shuttle vector pRM1
ORGANISM Shuttle vector pRM1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9021)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9021
/organism="Shuttle vector pRM1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4345."
/db_xref="taxon:1945227"
CDS complement(517..1638)
/transl_table=11
/note="Candida LEU2"
/protein_id="SJL87732.1"
/translation="MSVKTQTITVLPGDHVGTEIVNEPTKVLKAIEAPTPYQKIQFDF
KHHLIGGAAIDATGVPLPDDALESAKSSDAVLLGAVGGPKWGTGTVRPEQGLLKIRKE
LNLYANIRPCNFASDSLLELSPLKAEVVKGTNLIIVRELVGGIYFGERQEQEESEDHQ
TAWYRPKYTVDEVTRITRMAAFMALQHNPPLPIWSLDKANVLASSRLWRKTVDKVISE
EFPALSVQHQLIDSAAMILIQNPTKLSGIIITSNMFGDIISDEASVIPGSLGLLPSAS
LASLPDTNTAFGLYEPCHGSAPDLPANKVNPIATILSAASMLRLALDCVKETEALEEA
VKQVLDSGIRTADLRGTSSTTEVGDAIAETVTKILKQNT"
rep_origin complement(2562..3230)
/note="Candida ARS2"
CDS complement(<4143..4952)
/transl_table=11
/note="Candida URA3"
/protein_id="SJL87733.1"
/translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTK
EFLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNT
VKKQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSV
GSLAYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLG
QQYRTVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"
rep_origin 6092..6732
/note="Candida ARS3"
regulatory complement(6843..6926)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(7018..7420)
/note="pMB1 ori"
CDS complement(7961..8821)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87734.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2596 a 1994 c 1830 g 2601 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaatt cagtcagttt attgacaacg aatttggaaa caagcccgtg cccaagttgt
241 cgaaacctct gttgcctcac aataccagcg ctataacatt gacgagcagt agtgaggaga
301 agagtttttc tctccgaatg aagagccaaa tcatatacga gtgccaagtc aatccgaatc
361 aagcgaggcc accattacat ctaccaggca aaaaaatttt gcaaataagc aacctttatt
421 gagtgaagat tcggtaactc agttgtgtgc atatttagag aaatctgtag actttggagt
481 atttgaaatt taaatgctaa ctactgtata tactgtttat gtattttgtt ttaaaatttt
541 agtgactgtt tcggctattg cgtcaccgac ttctgtagta gagctagtac ctcttaaatc
601 tgctgttctg ataccgctat ccaaaacctg tttgacagct tcttccaatg cttcagtttc
661 cttaacacaa tccaaggcta atctcaacat ggaagcggca gacaagattg tagcaattgg
721 attcactttg ttggctggta aatctggagc agacccatgg caaggctcat aaagaccaaa
781 agcagtgttg gtatctggca atgaagccaa tgaagcagat ggcaataaac ccaaagatcc
841 tgggataacg gatgcctcat ctgagatgat atcaccaaac atgttagatg taatgatgat
901 accgctcaat ttggttgggt tttggatcaa aatcattgca gccgaatcaa tcaattgatg
961 ctgcacagac aaagctggaa attcttcaga aatcacttta tcaacggtct ttctccacaa
1021 tctcgacgat gccaaaacat tagctttgtc caaagaccat attggcaatg gtggattgtg
1081 ctgcaaagcc ataaatgctg ccatacgagt gatacgagta acttcgtcaa cagtatactt
1141 tggtctatac caagctgttt ggtggtcttc actttcttct tgttcttgac gttcaccaaa
1201 atatatacca ccaactaatt cacgaacaat aattagattg gttcctttga ccacttcagc
1261 ttttaatgga gacaattcta ataacgaatc actggcaaag ttacatggtc taatgttggc
1321 ataaaggttc aattctttac ggattttcaa caatccttgt tcaggacgaa cagtgccagt
1381 accccatttg ggtccaccaa cagcacccaa caaaactgca tcggatgatt tagcactttc
1441 aagagcatca tctggaagcg ggacgccagt ggcatcaatg gcagcaccac caatcaagtg
1501 gtgtttgaaa tcaaattgga ttttttggta gggagtggga gcttcaattg ctttcaagac
1561 tttggttggt tcattgacaa tttcggtacc gacatggtca ccaggcaaga cagtaatggt
1621 ttgggtttta acagacatgt ttttgggata ttggttttaa aagaaaggaa taagaaaagg
1681 ggagtatttc tggagtgaaa aaaaaaaatt catttgttga gaaaagggca ccccagaagg
1741 agcgccggaa tagcaaaatc acaacctcat gctcgctccg gtacaggcaa aaaaaaaaaa
1801 ttattcaaag taatctcttt ctatacatga taaatacacc aacaaaaact acctaatgac
1861 cactccattc aattgtattc aaatcaatac cttcttgaac catttcccat aacggggaca
1921 tttttcttaa aaagtcaact ctttcattaa ttgcttgaat gacatagtcg acttcggctt
1981 cagttgtaaa tctaccaatc ccaaatctaa ttgaagaatg agccaaggca tcatcagctc
2041 ccaatgcatg caaaacatat gatggttcca aagatgccga agtacatgct gacccagaac
2101 tcaaagcaat atctttcaag gccattaaca acgattcacc ttcaatgtaa gcaaaagata
2161 cattcacgca accagggtat tgagatttgg cgtcgttaca accgttaaat tgggtagatg
2221 gaattgacaa caatccattt ttcaacttag aggataattt ctcaatgtgt cttttgtcaa
2281 acagcgattc ttgcttcatt aatcttgctg cttcaccaaa cccagcaact aatggaggag
2341 ctaaagtacc tgaacgcaaa cctctttctt gaccaccacc agtaatgatt ggatctatgc
2401 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc gatagattgt
2461 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
2521 gttggaattt aatcgcggcc gctacgaatg ttagagacaa aatgtagtcc aggaccggcc
2581 agacgcagtg tgcgtgctgg ttgtgcaatt atactagcac atctaggtgt tttaggaaga
2641 aacgtccacc accaaaaaat taatttccaa atattggcac ttttattaca ccagtggtgt
2701 tacacagccc cccaaacact atcccggttg ttagattgaa gtttgtttaa caaaaattgg
2761 aagttctatt ttttactttt tgtacaaaat ttggcaagaa aattggagta aaatattttt
2821 atataaattt caaactaaaa atatccaaaa cataaaataa tacacaaaat gagatgctct
2881 atttagccaa accaaccatt acgggtgtgt tgtttggtgt tgtctgacca tgggtatacc
2941 atttgttagt gtatagctgc actgttttat gtttttcgat ttttccacat aaaaggagta
3001 ctcctatact aataacaaac actccactat aattggcaac cacaattatg cctggggaaa
3061 tattttccac tccgagtgta catacatttt tggtgtgttg ttggtggttg aaaattgtgt
3121 gggtcacgtg catttaatta gggttagggc tacggtttgt gtgcagtacc acgttcaaat
3181 tgaggctctc tggtataact tgcactacct catcgctacc ccggattttt ttttggtatg
3241 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
3301 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
3361 gcttgtttcg gcgtgggtaa ggtggcaggc tcggtggccg ggggactgtt gggcgccatc
3421 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
3481 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag caagacgttt cccgttgaat
3541 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
3601 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
3661 ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc
3721 agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc
3781 tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta
3841 tgagtcagca acaccttctt cacgaggcag acctcagcgc tattctgacc ttgccatcac
3901 gactgtgctg gtcattaaac gcgtattcag gctgaccctg cgcgctgcgc agggctttat
3961 tgattccatt tttacactga tgaatgttcc gttgcgctgc ccggattaca gctagaagga
4021 ccacctttga ttgtaaatag taataattac cacccttatt taattattta tttaacttat
4081 ttatttattt attctacata tatacaaatc taataaagtg aaaatctccc ccttcacatt
4141 tataattggc cagtcttttt caaataagca ttccaaccag catttctata ccttttacct
4201 tcaatatctg gatctcttcc tttaccaaac aatcctctac caacaatgat aatatcagtt
4261 ccagtgctaa caacttcatc aacagttcta tattgttgtc ctaatccatc acctttatca
4321 tctaatccaa ctccaggtgt cataataagc caatcaaatc cttcttcttg gccacccata
4381 tcacgttggg caataaatcc aataacaaat tccttatcgg atttagcaat ttcaacagtt
4441 ttttgagaat attctccata tgctaatgat cccactgatg ataattcagc taacatcaat
4501 aaccctcttg gctcttggtt ggtggtggtt tctttagctc cctgttttaa tccttcaacc
4561 actccattcc cagtgacacc atgagcattg gtaatatctg cccaactact aattttataa
4621 actccaccaa tatattgttt ctttacggta ttaccaatat cagcaaattt tctatcttca
4681 aaaatcataa attgatgttt acgtgaaagt tctaataatg gttcaatagt ggattcatag
4741 gaaaaatcat tgattatatc aatatgagtc ttgattaagc atacataagg acctaattta
4801 tcaattaatt caaggaattc cttagtggta tcaacgtcaa ttgatgcaca taaattggtt
4861 ttcttcagtt ccattaatcg aaataatcgc tgtgctactg gtgaggcatg agtttctgct
4921 ctctcactat aggtcttagt gttgactgtc atatcttttg tttgctattt ctatttctat
4981 ttctttcttc aactgtaaaa ggggcggtat ataaaccagt gtgtatgggg ttgttgctca
5041 ggaattgatc aatgtttaaa agaatggttg aaatcatcat tgaagaacaa aaaaaaagat
5101 tttttctttc attatgttat ctgtataata tgcaggaaat aagattgcaa aaaaatagcc
5161 gtttttttgg atactatcaa acaagaggaa tgactaatac agaattagtg ttacgaatca
5221 atggcactac agcaactttc aacaaaataa tctacgttca aaatataata tatactatta
5281 aataaaatca aataataaaa taattttaaa gaaagtagta ccagctcttt tttttgtttc
5341 cgtttatacc atccaaatca attcctatta gtactcaacc aagtcattct gagaatagtg
5401 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
5461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
5521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
5581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
5641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
5701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
5761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
5821 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
5881 tcaagaattc tcatgtttga cagcttatca tcgcgccatt cgccattcag gctgcgcaac
5941 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
6001 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
6061 acgacggcca gtgaattccc ggggatccag acttgttaaa tctgatgttt tgacgattat
6121 cattgaatgt aagatcaata aactattctc tttcttttta tttcaaaacc ttgcagtacc
6181 atggaattat atcaagccat gttttaaatt gatcagtagt ctaatcgggc ttcaagggaa
6241 aaggtggttt actcgcttaa cgatactttt gacatttcta ctacttagat tgtaagagag
6301 cattggtagc atcaaaaaca cacaaaatat aatcattttc ccaattggga acaattcaaa
6361 ataaaaacaa ctttgatgcg aagaacttca atattttagc taattatatc aaaattggaa
6421 tggagtctct actactacat gcttttctca atgaaattac gttaaaactt ttctcgtctt
6481 cttccatttt tttttatatt ctttactttt tctttaattt ttttttgtca ttccttattt
6541 aaatttccag aatacccttt aaactacata atatcatgaa gaatccattt gacagtggca
6601 gtgacgatga agatccattt cttagtaatc cacaatctgc accatcaatg ccctacgcag
6661 catatactag tgacagaaca tcgccccgca agacatacca accattgaat tttgacagtg
6721 aggacgaaga tgctaaagaa agcgaattta tggctttccc actgtcgacc tgcaggcatg
6781 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
6841 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
6901 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
6961 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
7021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
7081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
7141 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
7201 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
7261 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
7321 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
7381 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
7441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
7501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
7561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
7621 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
7681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
7741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
7801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
7861 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
7921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
7981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
8041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
8101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
8161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
8221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
8281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
8341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
8401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
8461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
8521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
8581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
8641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
8701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
8761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
8821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
8881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
8941 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
9001 gtatcacgag gccctttcgt c
//