LOCUS LT727189 5487 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV25ShTNFR75m5, complete sequence.
ACCESSION LT727189
VERSION LT727189.1
KEYWORDS .
SOURCE Mammalian expression vector pSV25ShTNFR75m5
ORGANISM Mammalian expression vector pSV25ShTNFR75m5
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5487)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5487
/organism="Mammalian expression vector pSV25ShTNFR75m5"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4334."
/db_xref="taxon:1945097"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL87716.1"
/translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE
NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA
VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL
CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR
RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ"
regulatory complement(810..906)
/note="polyA"
/regulatory_class="terminator"
regulatory 810..906
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(907..1458)
/note="3UTR"
misc_feature complement(1459..1525)
/note="intron"
CDS complement(1526..>1600)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87717.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
CDS complement(1620..2828)
/transl_table=11
/note="R75m5"
/protein_id="SJL87718.1"
/translation="MPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVF
CTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGW
YCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPH
QICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTS
FLLPMGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREA
KVPHLPADKARGTQGPEQQHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEAS
GAGEARASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTDSSPSE
SPKDEQVPFS"
misc_feature complement(2829..2894)
/note="sTNFR"
rep_origin complement(2941..3016)
/note="SV40 ori"
regulatory complement(2973..3254)
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin complement(3476..3878)
/note="pMB1 ori"
CDS complement(4419..5279)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87719.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1355 a 1316 c 1417 g 1399 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtacct
1621 taggagaagg ggacctgctc gtccttcggg gactccgagg ggctggaatc tgtgtctccc
1681 attgtggagc tggcttggga ggagcactgt gagctgtggt cagagctgct acagacgttc
1741 acgatgcagg tgacattgac ctgggtccca tggccaccag gggaagaatc tgagctcccg
1801 gtgctggccc gggcctcccc ggccccactg gcctccacgc ctggtgcctg tggctggttc
1861 cgagtgggcg cccttctgtc caacgcactg gccgagctct ccagggagct gctgctggag
1921 ctcggcgctg tgatcagcag gtgctgctgc tcggggccct gtgtaccccg ggccttatcg
1981 gcaggcaagt gaggcacctt ggcttctctc tgcaggcaca agggcttctt tttcacctgg
2041 gtcatgatga cacagttcac cactcctatt attagtagac ccaaggctgt cacacccaca
2101 atcagtccaa ctggaagagc gaagtcgcca gtgctccctt cagctggggg gctggggccc
2161 attgggagca ggaaggaggt gcttggagca gtgctgggtt ctggagttgg ctgcgtgtgt
2221 tgggatcgtg tggacactgg ctggggtaag tgtactgccc ctggggccat actccgggtg
2281 ggggacgtgg acgtgcagac tgcatccatg cttgcattcc cagggatggc caccacgtta
2341 cagatctggt ggggcctgca aatatccgtg gatgaagtcg tgttggagaa cgtccccggg
2401 gcacagggct tgcacaccac gtctgatgtt tcagttcctg gtctggccac gccgaagccc
2461 gggcggcact tgcgcagcgg cgcgcacagc cggcacccct cctgcttgct cagcgcgcag
2521 taccagccgg gcctgcaggt gcagatgcgg ttctgttccc gagtgcaggc ttgagtttcc
2581 acctggtcag agctacagcg ggagccacag ctcaagcact cgggaaccca gttccagagc
2641 tgggtgtatg tgctgtcctc acaggagtca cacacggtgt ccgaggtctt ggtacagaag
2701 acttttgcat gttggcccgg cgagcatttg ctgcagcaca tctgagctgt ctggtcatag
2761 tattctctga gccggcatgt gctcccgggc tccggggcgt agggtgtaaa tgccacctgg
2821 gcgggcaagg cgtgcgccgc agcccagagc tccagtccga cggccagcgc ggcccagacg
2881 gcgacgggcg ccatggtggc ggcaagcttg agctttttgc aaaagcctag gcctccaaaa
2941 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
3001 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
3061 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
3121 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
3181 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
3241 cacacattcc acagtcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
3301 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
3361 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
3421 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgctct
3481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
3541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
3601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
3661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
3721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
3781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
3841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
3901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
3961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4081 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
4141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
4201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
4261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
4321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
4381 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
4441 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
4501 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
4561 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
4621 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
4681 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
4741 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
4801 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
4861 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
4921 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
4981 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
5041 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
5101 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
5161 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
5221 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
5281 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
5341 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
5401 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
5461 atcacgaggc cctttcgtct tcaagaa
//