LOCUS LT727188 5439 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV25ShTNFR75m4, complete sequence.
ACCESSION LT727188
VERSION LT727188.1
KEYWORDS .
SOURCE Mammalian expression vector pSV25ShTNFR75m4
ORGANISM Mammalian expression vector pSV25ShTNFR75m4
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5439)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5439
/organism="Mammalian expression vector pSV25ShTNFR75m4"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4333."
/db_xref="taxon:1945096"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL87712.1"
/translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE
NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA
VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL
CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR
RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ"
regulatory complement(810..906)
/note="polyA"
/regulatory_class="terminator"
regulatory 810..906
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(907..1458)
/note="3UTR"
misc_feature complement(1459..1525)
/note="intron"
CDS complement(1526..>1600)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87713.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
CDS complement(1620..2780)
/transl_table=11
/note="R75m4"
/protein_id="SJL87714.1"
/translation="MPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVF
CTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGW
YCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPH
QICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTS
FLLPMGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREA
KVPHLPADKARASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTD
SSPSESPKDEQVPFSKEECAFRSQLETPETLLGSTEEKPLPLGVPDAGMKPS"
misc_feature complement(2781..2846)
/note="sTNFR"
rep_origin complement(2893..2968)
/note="SV40 ori"
regulatory complement(2925..3206)
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin complement(3430..3830)
/note="pMB1 ori"
CDS complement(4371..5231)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87715.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1359 a 1295 c 1393 g 1392 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtacct
1621 taactgggct tcatcccagc atcaggcact ccaaggggca ggggcttctc ttcggtgctc
1681 cccagcaggg tctctggcgt ctccagctgt gaccgaaagg cacattcctc cttggagaag
1741 gggacctgct cgtccttcgg ggactccgag gggctggaat ctgtgtctcc cattgtggag
1801 ctggcttggg aggagcactg tgagctgtgg tcagagctgc tacagacgtt cacgatgcag
1861 gtgacattga cctgggtccc atggccacca ggggaagaat ctgagctccc ggtgctggcc
1921 cgggccttat cggcaggcaa gtgaggcacc ttggcttctc tctgcaggca caagggcttc
1981 tttttcacct gggtcatgat gacacagttc accactccta ttattagtag acccaaggct
2041 gtcacaccca caatcagtcc aactggaaga gcgaagtcgc cagtgctccc ttcagctggg
2101 gggctggggc ccattgggag caggaaggag gtgcttggag cagtgctggg ttctggagtt
2161 ggctgcgtgt gttgggatcg tgtggacact ggctggggta agtgtactgc ccctggggcc
2221 atactccggg tgggggacgt ggacgtgcag actgcatcca tgcttgcatt cccagggatg
2281 gccaccacgt tacagatctg gtggggcctg caaatatccg tggatgaagt cgtgttggag
2341 aacgtccccg gggcacaggg cttgcacacc acgtctgatg tttcagttcc tggtctggcc
2401 acgccgaagc ccgggcggca cttgcgcagc ggcgcgcaca gccggcaccc ctcctgcttg
2461 ctcagcgcgc agtaccagcc gggcctgcag gtgcagatgc ggttctgttc ccgagtgcag
2521 gcttgagttt ccacctggtc agagctacag cgggagccac agctcaagca ctcgggaacc
2581 cagttccaga gctgggtgta tgtgctgtcc tcacaggagt cacacacggt gtccgaggtc
2641 ttggtacaga agacttttgc atgttggccc ggcgagcatt tgctgcagca catctgagct
2701 gtctggtcat agtattctct gagccggcat gtgctcccgg gctccggggc gtagggtgta
2761 aatgccacct gggcgggcaa ggcgtgcgcc gcagcccaga gctccagtcc gacggccagc
2821 gcggcccaga cggcgacggg cgccatggtg gcggcaagct tgagcttttt gcaaaagcct
2881 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
2941 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
3001 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
3061 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
3121 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
3181 caccctaact gacacacatt ccacagtcgg gcagcgttgg gtcctggcca cgggtgcgca
3241 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
3301 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
3361 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
3421 agtcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
3481 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
3541 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
3601 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
3661 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
3721 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
3781 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
3841 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
3901 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
3961 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
4021 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
4081 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
4141 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
4201 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
4261 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
4321 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
4381 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
4441 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
4501 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
4561 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
4621 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
4681 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
4741 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
4801 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
4861 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
4921 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
4981 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
5041 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
5101 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
5161 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
5221 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
5281 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
5341 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
5401 aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//