LOCUS LT727187 5472 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV25ShTNFR75m3, complete sequence.
ACCESSION LT727187
VERSION LT727187.1
KEYWORDS .
SOURCE Mammalian expression vector pSV25ShTNFR75m3
ORGANISM Mammalian expression vector pSV25ShTNFR75m3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5472)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5472
/organism="Mammalian expression vector pSV25ShTNFR75m3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4332."
/db_xref="taxon:1945095"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL87708.1"
/translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE
NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA
VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL
CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR
RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ"
regulatory complement(810..906)
/note="polyA"
/regulatory_class="terminator"
regulatory 810..906
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(907..1458)
/note="3UTR"
misc_feature complement(1459..1525)
/note="intron"
CDS complement(1526..>1600)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87709.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
CDS complement(1620..2813)
/transl_table=11
/note="R75m3"
/protein_id="SJL87710.1"
/translation="MPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVF
CTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGW
YCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPH
QICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTS
FLLPMGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREA
KVPHLPADKARGTQGPEQQHLLITAPSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCS
SQASSTMGDTDSSPSESPKDEQVPFSKEECAFRSQLETPETLLGSTEEKPLPLGVPDA
GMKPS"
misc_feature complement(2814..2879)
/note="sTNFR"
rep_origin complement(2926..3001)
/note="SV40 ori"
regulatory complement(2958..3239)
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin complement(3460..3860)
/note="pMB1 ori"
CDS complement(4404..5264)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87711.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1363 a 1304 c 1405 g 1400 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtacct
1621 taactgggct tcatcccagc atcaggcact ccaaggggca ggggcttctc ttcggtgctc
1681 cccagcaggg tctctggcgt ctccagctgt gaccgaaagg cacattcctc cttggagaag
1741 gggacctgct cgtccttcgg ggactccgag gggctggaat ctgtgtctcc cattgtggag
1801 ctggcttggg aggagcactg tgagctgtgg tcagagctgc tacagacgtt cacgatgcag
1861 gtgacattga cctgggtccc atggccacca ggggaagaat ctgagctcgg cgctgtgatc
1921 agcaggtgct gctgctcggg gccctgtgta ccccgggcct tatcggcagg caagtgaggc
1981 accttggctt ctctctgcag gcacaagggc ttctttttca cctgggtcat gatgacacag
2041 ttcaccactc ctattattag tagacccaag gctgtcacac ccacaatcag tccaactgga
2101 agagcgaagt cgccagtgct cccttcagct ggggggctgg ggcccattgg gagcaggaag
2161 gaggtgcttg gagcagtgct gggttctgga gttggctgcg tgtgttggga tcgtgtggac
2221 actggctggg gtaagtgtac tgcccctggg gccatactcc gggtggggga cgtggacgtg
2281 cagactgcat ccatgcttgc attcccaggg atggccacca cgttacagat ctggtggggc
2341 ctgcaaatat ccgtggatga agtcgtgttg gagaacgtcc ccggggcaca gggcttgcac
2401 accacgtctg atgtttcagt tcctggtctg gccacgccga agcccgggcg gcacttgcgc
2461 agcggcgcgc acagccggca cccctcctgc ttgctcagcg cgcagtacca gccgggcctg
2521 caggtgcaga tgcggttctg ttcccgagtg caggcttgag tttccacctg gtcagagcta
2581 cagcgggagc cacagctcaa gcactcggga acccagttcc agagctgggt gtatgtgctg
2641 tcctcacagg agtcacacac ggtgtccgag gtcttggtac agaagacttt tgcatgttgg
2701 cccggcgagc atttgctgca gcacatctga gctgtctggt catagtattc tctgagccgg
2761 catgtgctcc cgggctccgg ggcgtagggt gtaaatgcca cctgggcggg caaggcgtgc
2821 gccgcagccc agagctccag tccgacggcc agcgcggccc agacggcgac gggcgccatg
2881 gtggcggcaa gcttgagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
2941 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
3001 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
3061 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
3121 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
3181 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagt
3241 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
3301 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
3361 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
3421 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gctcttccgc ttcctcgctc
3481 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
3541 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
3601 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
3661 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
3721 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
3781 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
3841 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
3901 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
3961 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
4021 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
4081 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
4141 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
4201 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
4261 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
4321 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
4381 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
4441 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
4501 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
4561 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
4621 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
4681 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
4741 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
4801 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
4861 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
4921 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
4981 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
5041 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
5101 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
5161 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
5221 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
5281 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
5341 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
5401 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
5461 cgtcttcaag aa
//