LOCUS LT727185 5613 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV25ShTNFR75m1, complete sequence.
ACCESSION LT727185
VERSION LT727185.1
KEYWORDS .
SOURCE Mammalian expression vector pSV25ShTNFR75m1
ORGANISM Mammalian expression vector pSV25ShTNFR75m1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5613)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5613
/organism="Mammalian expression vector pSV25ShTNFR75m1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4330."
/db_xref="taxon:1945093"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL87700.1"
/translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE
NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA
VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL
CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR
RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ"
regulatory complement(810..906)
/note="polyA"
/regulatory_class="terminator"
regulatory 810..906
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(907..1458)
/note="3UTR"
misc_feature complement(1459..1525)
/note="intron"
CDS complement(1526..>1600)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87701.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
CDS complement(1998..2954)
/transl_table=11
/note="R75m1"
/protein_id="SJL87702.1"
/translation="MPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVF
CTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGW
YCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPH
QICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTS
FLLPMGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREA
KVPHLPADKARGTQGPEQQHLLITAPSSSSSSLESSATSRGL"
misc_feature complement(2955..3020)
/note="sTNFR"
rep_origin complement(3067..3142)
/note="SV40 ori"
regulatory complement(3099..3380)
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin complement(3605..4005)
/note="pMB1 ori"
CDS complement(4545..5405)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87703.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1376 a 1357 c 1452 g 1428 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtacct
1621 taactgggct tcatcccagc atcaggcact ccaaggggca ggggcttctc ttcggtgctc
1681 cccagcaggg tctctggcgt ctccagctgt gaccgaaagg cacattcctc cttggagaag
1741 gggacctgct cgtccttcgg ggactccgag gggctggaat ctgtgtctcc cattgtggag
1801 ctggcttggg aggagcactg tgagctgtgg tcagagctgc tacagacgtt cacgatgcag
1861 gtgacattga cctgggtccc atggccacca ggggaagaat ctgagctccc ggtgctggcc
1921 cgggcctccc cggccccact ggcctccacg cctggtgcct gtggctggtt ccgagtgggc
1981 gcccttctgt ccaacctcta gaggcctcta gaggtggccg agctctccag ggagctgctg
2041 ctggagctcg gcgctgtgat cagcaggtgc tgctgctcgg ggccctgtgt accccgggcc
2101 ttatcggcag gcaagtgagg caccttggct tctctctgca ggcacaaggg cttctttttc
2161 acctgggtca tgatgacaca gttcaccact cctattatta gtagacccaa ggctgtcaca
2221 cccacaatca gtccaactgg aagagcgaag tcgccagtgc tcccttcagc tggggggctg
2281 gggcccattg ggagcaggaa ggaggtgctt ggagcagtgc tgggttctgg agttggctgc
2341 gtgtgttggg atcgtgtgga cactggctgg ggtaagtgta ctgcccctgg ggccatactc
2401 cgggtggggg acgtggacgt gcagactgca tccatgcttg cattcccagg gatggccacc
2461 acgttacaga tctggtgggg cctgcaaata tccgtggatg aagtcgtgtt ggagaacgtc
2521 cccggggcac agggcttgca caccacgtct gatgtttcag ttcctggtct ggccacgccg
2581 aagcccgggc ggcacttgcg cagcggcgcg cacagccggc acccctcctg cttgctcagc
2641 gcgcagtacc agccgggcct gcaggtgcag atgcggttct gttcccgagt gcaggcttga
2701 gtttccacct ggtcagagct acagcgggag ccacagctca agcactcggg aacccagttc
2761 cagagctggg tgtatgtgct gtcctcacag gagtcacaca cggtgtccga ggtcttggta
2821 cagaagactt ttgcatgttg gcccggcgag catttgctgc agcacatctg agctgtctgg
2881 tcatagtatt ctctgagccg gcatgtgctc ccgggctccg gggcgtaggg tgtaaatgcc
2941 acctgggcgg gcaaggcgtg cgccgcagcc cagagctcca gtccgacggc cagcgcggcc
3001 cagacggcga cgggcgccat ggtggcggca agcttgagct ttttgcaaaa gcctaggcct
3061 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
3121 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
3181 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
3241 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
3301 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
3361 aactgacaca cattccacag tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
3421 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
3481 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
3541 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
3601 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3661 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3721 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3781 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3841 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3901 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3961 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
4021 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
4081 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
4141 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
4201 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
4261 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
4321 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
4381 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4441 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4501 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4561 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4621 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4681 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4741 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4801 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4861 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4921 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4981 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
5041 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
5101 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
5161 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
5221 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
5281 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
5341 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
5401 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5461 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5521 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
5581 aggcgtatca cgaggccctt tcgtcttcaa gaa
//