LOCUS LT727184 6750 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1mBID, complete sequence.
ACCESSION LT727184
VERSION LT727184.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1mBID
ORGANISM Mammalian expression vector pEF1mBID
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6750)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6750
/organism="Mammalian expression vector pEF1mBID"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4325."
/db_xref="taxon:1944982"
regulatory 508..1651
/note="hEF1 promoter"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron hEF1 promoter"
regulatory 1668..1684
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1754..2341
/transl_table=11
/note="mBID"
/protein_id="SJL87697.1"
/translation="MDSEVSNGSGLGAKHITDLLVFGFLQSSGCTRQELEVLGRELPV
QAYWEADLEDELQTDGSQASRSFNQGRIEPDSESQEEIIHNIARHLAQIGDEMDHNIQ
PTLVRQLAAQFMNGSLSEEDKRNCLAKALDEVKTAFPRDMENDKAMLIMTMLLAKKVA
SHAPSLLRDVFHTTVNFINQNLFSYVRNLVRNEMD"
misc_feature 2381..2422
/note="V5 epitope"
misc_feature 2432..2449
/note="His-tag"
regulatory 2478..2702
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 2748..3176
/note="f1 ori"
regulatory 3181..3462
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3419..3494
/note="SV40 ori"
CDS 3586..4380
/transl_table=11
/note="neomycin"
/protein_id="SJL87698.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory complement(4556..4630)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 4556..4630
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4769..4852)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4944..5335)
/note="pMB1 ori"
CDS complement(5876..6736)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87699.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1492 a 1763 c 1832 g 1663 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
1741 tgtggtggaa ttcatggact ctgaggtcag caacggttcc ggcctggggg ccaagcacat
1801 cacagacctg ctggtgttcg gctttctcca aagctctggc tgtactcgcc aagagctgga
1861 ggtgctgggt cgggaactgc ctgtgcaagc ttactgggag gcagacctcg aagacgagct
1921 gcagacagac ggcagccagg ccagccgctc cttcaaccaa ggaagaatag agccagattc
1981 tgaaagtcag gaagaaatca tccacaacat tgccagacat ctcgcccaaa taggcgatga
2041 gatggaccac aacatccagc ccacactggt gagacagcta gccgcacagt tcatgaatgg
2101 cagcctgtcg gaggaagaca aaaggaactg cctggccaaa gcccttgatg aggtgaagac
2161 agccttcccc agagacatgg agaacgacaa ggccatgctg ataatgacaa tgctgttggc
2221 caaaaaagtg gccagtcacg caccatcttt gctccgtgat gtcttccaca cgactgtcaa
2281 ctttattaac cagaacctat tctcctatgt gaggaacttg gttagaaacg agatggactg
2341 atccagcaca gtggcggccg ctcgaggtca cccattcgaa ggtaagccta tccctaaccc
2401 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac
2461 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
2521 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
2581 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
2641 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
2701 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
2761 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
2821 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
2881 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
2941 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
3001 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
3061 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
3121 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
3181 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
3241 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
3301 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
3361 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
3421 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
3481 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
3541 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
3601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
3661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
3721 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
3781 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
3841 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
3901 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
3961 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
4021 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
4081 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
4141 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
4201 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
4261 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
4321 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
4381 gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
4441 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
4501 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
4561 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
4621 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
4681 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
4741 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
4801 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
4861 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4921 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
5041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccaggaac cgtaaaaagg
5101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
5161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
5221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
5281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
5341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
5401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
5461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
5521 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
5581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
5641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
5701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
5761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
5821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
5881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
5941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
6001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
6061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
6121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
6181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
6241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
6301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
6361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
6421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
6481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
6541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
6601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
6661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
6721 aatgttgaat actcatactc ttcctttttc
//