LOCUS LT727182 9886 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMC1403, complete sequence.
ACCESSION LT727182
VERSION LT727182.1
KEYWORDS .
SOURCE Vector pMC1403
ORGANISM Vector pMC1403
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9886)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9886
/organism="Vector pMC1403"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 474."
/db_xref="taxon:1945344"
CDS <15..3062
/codon_start=1
/transl_table=11
/note="lacZ"
/protein_id="SJL87690.1"
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVN
PPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLP
SEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGG
YADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLL
LLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYT
LCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQS
LIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLS
GQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPES
AGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFC
IELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAW
VERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQM
AITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPL
SDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEE
GTWLNIDGFHMGIGGDDSWSPSVSAXXQLSAGRYHYQLVWCQK"
CDS 3114..4367
/transl_table=11
/note="lacY"
/protein_id="SJL87691.1"
/translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
TLISVFTLSGPGPLSLLRRQVNEVA"
CDS 4431..5042
/transl_table=11
/note="lacA"
/protein_id="SJL87692.1"
/translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
rep_origin complement(7870..8280)
/note="pMB1 ori"
CDS complement(8818..9678)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87693.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2305 a 2655 c 2588 g 2332 t
ORIGIN
1 ttcccgggga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
61 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
121 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
181 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
241 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa
301 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
361 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
421 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
481 acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc
541 tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc
601 ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg
661 atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag
721 ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg
781 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg
841 gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg
901 aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg
961 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc
1021 tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg
1081 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact
1141 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc
1201 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
1261 atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg
1321 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc
1381 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg
1441 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg
1501 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc
1561 tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta
1621 acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg
1681 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc
1741 cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga
1801 acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc
1861 agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt
1921 tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg
1981 caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg
2041 aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga
2101 acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg
2161 aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg
2221 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct
2281 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt
2341 tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
2401 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc
2461 agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc
2521 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa
2581 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
2641 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
2701 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
2761 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
2821 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
2881 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
2941 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
3001 cggcgnnnnn ncagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
3061 aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact
3121 atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac ttttttatca
3181 tgggagccta cttcccgttt ttcccgattt ggctacatga catcaaccat atcagcaaaa
3241 gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc caaccgctgt
3301 ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt attaccggca
3361 tgttagtgat gtttgcgccg ttctttattt ttatcttcgg gccactgtta caatacaaca
3421 ttttagtagg atcgattgtt ggtggtattt atctaggctt ttgttttaac gccggtgcgc
3481 cagcagtaga ggcatttatt gagaaagtca gccgtcgcag taatttcgaa tttggtcgcg
3541 cgcggatgtt tggctgtgtt ggctgggcgc tgtgtgcctc gattgtcggc atcatgttca
3601 ccatcaataa tcagtttgtt ttctggctgg gctctggctg tgcactcatc ctcgccgttt
3661 tactcttttt cgccaaaacg gatgcgccct cttctgccac ggttgccaat gcggtaggtg
3721 ccaaccattc ggcatttagc cttaagctgg cactggaact gttcagacag ccaaaactgt
3781 ggtttttgtc actgtatgtt attggcgttt cctgcaccta cgatgttttt gaccaacagt
3841 ttgctaattt ctttacttcg ttctttgcta ccggtgaaca gggtacgcgg gtatttggct
3901 acgtaacgac aatgggcgaa ttacttaacg cctcgattat gttctttgcg ccactgatca
3961 ttaatcgcat cggtgggaaa aacgccctgc tgctggctgg cactattatg tctgtacgta
4021 ttattggctc atcgttcgcc acctcagcgc tggaagtggt tattctgaaa acgctgcata
4081 tgtttgaagt accgttcctg ctggtgggct gctttaaata tattaccagc cagtttgaag
4141 tgcgtttttc agcgacgatt tatctggtct gtttctgctt ctttaagcaa ctggcgatga
4201 tttttatgtc tgtactggcg ggcaatatgt atgaaagcat cggtttccag ggcgcttatc
4261 tggtgctggg tctggtggcg ctgggcttca ccttaatttc cgtgttcacg cttagcggcc
4321 ccggcccgct ttccctgctg cgtcgtcagg tgaatgaagt cgcttaagca atcaatgtcg
4381 gatgcggcgc gacgcttatc cgaccaacat atcataacgg agtgatcgca ttgaacatgc
4441 caatgaccga aagaataaga gcaggcaagc tatttaccga tatgtgcgaa ggcttaccgg
4501 aaaaaagact tcgtgggaaa acgttaatgt atgagtttaa tcactcgcat ccatcagaag
4561 ttgaaaaaag agaaagcctg attaaagaaa tgtttgccac ggtaggggaa aacgcctggg
4621 tagaaccgcc tgtctatttc tcttacggtt ccaacatcca tataggccgc aatttttatg
4681 caaatttcaa tttaaccatt gtcgatgact acacggtaac aatcggtgat aacgtactga
4741 ttgcacccaa cgttactctt tccgttacgg gacaccctgt acaccatgaa ttgagaaaaa
4801 acggcgagat gtactctttt ccgataacga ttggcaataa cgtctggatc ggaagtcatg
4861 tggttattaa tccaggcgtc accatcgggg ataattctgt tattggcgcg ggtagtatcg
4921 tcacaaaaga cattccacca aacgtcgtgg cggctggcgt tccttgtcgg gttattcgcg
4981 aaataaacga ccgggataag cactattatt tcaaagatta taaagttgaa tcgtcagttt
5041 aaattataaa aattgcctga tacgctgcgc ttatcaggcc tacaagttca gcgatctaca
5101 ttagccgcat ccggcatgaa caaagcgcag gaacaagcgt cgcatcatgc ctctttgacc
5161 cacagctgcg gaaaacgtac tggtgcaaaa cgcagggtta tgatcatcag cccaacgacg
5221 cacagcgcat gaaatgccca gtccatcagg taattgccgc tgatactacg cagcacgcca
5281 gaaaaccacg gggcaagccc ggcgatgata aaaccgattc cctgcataaa cgccaccagc
5341 ttgccagcaa tagccggttg cacagagtga tcgagcgcca gcagcaaaca gagcggaaac
5401 gcgccgccca gacctaaccc acacaccatc gcccacaata ccggcaattg catcggcagc
5461 cagataaagc cgcagaaccc caccagttgt aacaccagcg ccagcattaa cagtttgcgc
5521 cgatcctgat ggcgagccat agcaggcatc agcaaagctc ctgcggcttg cccaagcgtc
5581 atcaatgcca gtaaggaacc gctgtactgc gcgctggcac caatctcaat atagaaagcg
5641 ggtaaccagg caatcaggct ggcgtaaccg ccgttaatca gaccgaagta aacacccagc
5701 gtccacgcgc ggggagtgaa taccacgcga accggagtgg ttgttgtctt gtgggaagag
5761 gcgacctcgc gggcgctttg ccaccaccag gcaaagagcg caacaacggc aggcagcgcc
5821 accaggcgag tgtttgatac caggtttcgc tatgttgaac taaccagggc gttatggcgg
5881 caccaagccc accgccgccc atcagagccg cggaccacag ccccatcacc agtggcgtgc
5941 gctgctgaaa ccgccgttta atcaccgaag catcaccgcc tgaatgatgc cgatccccac
6001 cccaccaagc agtgcgctgc taagcagcag cgcactttgc gggtaaagct cacgcatcaa
6061 tgcaccgacg gcaatcagca acagactgat ggcgacactg cgacgttcgc tgacatgctg
6121 atgaagccag cttccggcca gcgccagccc gcccatggta accaccggca gagcggtcga
6181 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
6241 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
6301 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
6361 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc
6421 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
6481 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
6541 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
6601 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact
6661 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
6721 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
6781 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
6841 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
6901 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
6961 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
7021 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
7081 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
7141 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
7201 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
7261 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
7321 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
7381 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
7441 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
7501 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
7561 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
7621 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
7681 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
7741 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
7801 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
7861 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
7921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
7981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
8041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
8101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
8161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
8221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
8281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
8341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
8401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
8461 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
8521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
8581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
8641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
8701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
8761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
8821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
8881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
8941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
9001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
9061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
9121 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
9181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
9241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
9301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
9361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
9421 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
9481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
9541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
9601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
9661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
9721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
9781 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
9841 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaa
//