LOCUS LT727181 5982 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector p1168hIL6mIgkB-luc+, complete sequence.
ACCESSION LT727181
VERSION LT727181.1
KEYWORDS .
SOURCE Reporter vector p1168hIL6mIgkB-luc+
ORGANISM Reporter vector p1168hIL6mIgkB-luc+
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5982)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5982
/organism="Reporter vector p1168hIL6mIgkB-luc+"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4736."
/db_xref="taxon:1945193"
misc_feature 32..672
/note="5' FR of hIL6"
regulatory 673..1208
/note="hIL6 promoter mutant"
/regulatory_class="promoter"
CDS 1256..2908
/transl_table=11
/note="LUCm"
/protein_id="SJL87688.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 2996..3092
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(2996..3092)
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(3301..3703)
/note="pMB1 ori"
CDS complement(4244..5104)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87689.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5236..5691
/note="f1 ori"
regulatory complement(5822..5975)
/note="synthetic polyA"
/regulatory_class="terminator"
BASE COUNT 1585 a 1429 c 1432 g 1536 t
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag
61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag
121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt
181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg
241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac
301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag
361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac
421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg
481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc
541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg
601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag
661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga
721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg
781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa
841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac
901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg
961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc
1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat
1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggac
1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc
1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga
1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc
2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
3181 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
3241 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
3301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
3601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
3661 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
3721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
3781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
3841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
3901 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
3961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
4021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
4081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
4141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
4201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
4261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
4321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
4381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
4441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
4501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
4561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
4621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
4681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
4741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
4801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
4861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
4921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
4981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
5041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
5101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
5161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
5221 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
5281 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
5341 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
5401 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
5461 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
5521 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
5581 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
5641 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc
5701 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
5761 agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg
5821 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
5881 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
5941 ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta
//