LOCUS LT727177 4664 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pAT153MIL2F2, complete sequence.
ACCESSION LT727177
VERSION LT727177.1
KEYWORDS .
SOURCE Vector pAT153MIL2F2
ORGANISM Vector pAT153MIL2F2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4664)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4664
/organism="Vector pAT153MIL2F2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4731."
/db_xref="taxon:1945241"
CDS 86..1276
/transl_table=11
/note="TET"
/protein_id="SJL87677.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(1647..2049)
/note="pMB1 ori"
misc_feature complement(2951..3332)
/note="3UTR"
CDS complement(3333..3842)
/transl_table=11
/note="mIL2"
/protein_id="SJL87678.1"
/translation="MYSMQLASCVTLTLVLLVNSAPTSSSTSSSTAEAQQQQQQQQQQ
QQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRH
VLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFC
QSIISTSPQ"
misc_feature complement(3843..3889)
/note="5UTR"
BASE COUNT 1090 a 1206 c 1176 g 1192 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg
1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
2881 tttgcgcaac gttgttgcca ttgctgcagg gggggggggg gggggggggt tttttttttt
2941 tttttttttt agaggagagc tttatttctt gaaaacactg attaacatag agttcacagg
3001 aataactgag aagttatttt agcgcttact ttgtgctgtc ctaaaaatga cagacatctg
3061 agcttattta tatttgaatc atctaaatac ttttattaat gagtctacct acacatgata
3121 tttaacaatt caatataata aataatttca gataaatagt ttaaaacatt tttgagccct
3181 tggggcttac aaaaagaatc tttaaagatc catatttatc atctgaagac tagtagttac
3241 aaaagatagt aaacaataca tccaaaaata aattaaagtt aaatatttaa ataaatagag
3301 agccttatgt gttgtaagca ggaggtacat agttattgag ggcttgttga gatgatgctt
3361 tgacagaagg ctatccatct cctcagaaag tccaccacag ttgctgactc atcatcgaat
3421 tggcactcaa atgtgttgtc agagcccttt agttttacaa cagttactct gatattgctg
3481 atgaaattct cagcatcttc caattgaaag cttttgcttt gagtcaaatc cagaacatgc
3541 cgcagaggtc caagttcatc ttctaggcac tgaagatctt tcaattctgt ggcctgcttg
3601 ggcaagtaaa atttgaaggt gagcatcctg gggagtttca ggttcctgta attctccatc
3661 ctgctcagga gctcctgtag gtccatcaac agctgctcca ggtgctgctg ctgctgctgc
3721 tgctgctgct gctgctgctg tgcttccgct gtagagcttg aagtggagct tgaagtgggt
3781 gcgctgttga caaggagcac aagtgtcaat gtgacacagg atgcgagctg catgctgtac
3841 atgcctgcag gacttgaggt cactgtgagg agtgattagc aagggtgatc cccccccccc
3901 cccccccccc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
3961 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
4021 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
4081 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
4141 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
4201 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
4261 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
4321 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
4381 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
4441 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
4501 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
4561 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
4621 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//