LOCUS LT727176 8089 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1-mRIP, complete sequence.
ACCESSION LT727176
VERSION LT727176.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1-mRIP
ORGANISM Mammalian expression vector pEF1-mRIP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8089)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8089
/organism="Mammalian expression vector pEF1-mRIP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4720."
/db_xref="taxon:1944984"
regulatory 508..1651
/note="hEF1 promoter"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron hEF1 promoter"
regulatory 1668..1684
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1731..3701
/transl_table=11
/note="mRIP1"
/protein_id="SJL87674.1"
/translation="MQPDMSLDNIKMASSDLLEKTDLDSGGFGKVSLCYHRSHGFVIL
KKVYTGPNRAEYNEVLLEEGKMMHRLRHSRVVKLLGIIIEEGNYSLVMEYMEKGNLMH
VLKTQIDVPLSLKGRIIVEAIEGMCYLHDKGVIHKDLKPENILVDRDFHIKIADLGVA
SFKTWSKLTKEKDNKQKEVSSTTKKNNGGTLYYMAPEHLNDINAKPTEKSDVYSFGIV
LWAIFAKKEPYENVICTEQFVICIKSGNRPNVEEILEYCPREIISLMERCWQAIPEDR
PTFLGIEEEFRPFYLSHFEEYVEEDVASLKKEYPDQSPVLQRMFSLQHDCVPLPPSRS
NSEQPGSLHSSQGLQMGPVEESWFSSSPEYPQDENDRSVQAKLQEEASYHAFGIFAEK
QTKPQPRQNEAYNREEERKRRVSHDPFAQQRARENIKSAGARGHSDPSTTSRGIAVQQ
LSWPATQTVWNNGLYNQHGFGTTGTGVWYPPNLSQMYSTYKTPVPETNIPGSTPTMPY
FSGPVADDLIKYTIFNSSGIQIGNHNYMDVGLNSQPPNNTCKEESTSRHQAIFDNTTS
LTDEHLNPIRENLGRQWKNCARKLGFTESQIDEIDHDYERDGLKEKVYQMLQKWLMRE
GTKGATVGKLAQALHQCCRIDLLNHLIRASQS"
misc_feature 3720..3761
/note="V5 epitope"
misc_feature 3771..3788
/note="His-tag"
regulatory 3817..4041
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 4087..4515
/note="f1 ori"
regulatory 4520..4801
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4758..4833
/note="SV40 ori"
CDS 4925..5719
/transl_table=11
/note="neomycin"
/protein_id="SJL87675.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory complement(5895..5969)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 5895..5969
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(6108..6191)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(6283..6674)
/note="pMB1 ori"
CDS complement(7215..8075)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87676.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1941 a 2022 c 2162 g 1964 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc atgcaaccag
1741 acatgtcctt ggacaatatt aagatggcat ccagtgacct gctggagaag acagacctag
1801 acagcggagg cttcgggaag gtgtccttgt gttaccacag aagccatgga tttgtcatcc
1861 tgaaaaaagt atacacaggg cccaaccgcg ctgagtacaa tgaggttctc ttggaagagg
1921 ggaagatgat gcacagactg agacacagtc gagtggtgaa gctactgggc atcatcatag
1981 aagaagggaa ctattcgctg gtgatggagt acatggagaa gggcaacctg atgcacgtgc
2041 taaagaccca gatagatgtc ccactttcat tgaaaggaag gataatcgtg gaggccatag
2101 aaggcatgtg ctacttacat gacaaaggtg tgatacacaa ggacctgaag cctgagaata
2161 tcctcgttga tcgtgacttt cacattaaga tagccgatct tggtgtggct tcctttaaga
2221 catggagcaa actgactaag gagaaagaca acaagcagaa agaagtgagc agcaccacta
2281 agaagaacaa tggtggtacc ctttactaca tggcacccga acacctgaat gacatcaatg
2341 caaagcccac ggagaagtcg gacgtgtaca gctttggcat tgtcctttgg gcaatatttg
2401 caaaaaagga gccctatgag aatgtcatct gtactgagca gttcgtgatc tgcataaaat
2461 ctgggaacag gccaaatgta gaggaaatcc ttgagtactg tccaagggag atcatcagcc
2521 tcatggagcg gtgctggcag gcgatcccag aagacaggcc aacatttctt ggcattgaag
2581 aagaatttag gcctttttac ttaagtcatt ttgaagaata tgtagaagag gatgtggcaa
2641 gtttaaagaa agagtatcca gatcaaagcc cagtgctgca gagaatgttt tcactgcagc
2701 atgactgtgt acccttacct ccgagcaggt caaattcaga acaacctgga tcgctgcaca
2761 gttcccaggg gctccagatg ggtcctgtgg aggagtcctg gttttcttcc tccccagagt
2821 acccacagga cgagaatgat cgcagtgtgc aggctaagct gcaagaggaa gccagctatc
2881 atgcttttgg aatatttgca gagaaacaga caaaaccgca gccaaggcag aatgaggctt
2941 acaacagaga ggaggaaagg aaacgaaggg tctctcatga cccctttgca cagcagagag
3001 ctcgtgagaa tattaagagt gcaggagcaa gaggtcattc tgatcccagc acaacgagtc
3061 gtggaattgc agtgcaacag ctgtcatggc cagccaccca aacagtttgg aacaatggat
3121 tgtataatca gcatggattt ggaactacag gtacaggagt ttggtatccg ccaaatctaa
3181 gccaaatgta tagtacttat aaaactccag tgccagagac caacataccg ggaagcacac
3241 ccaccatgcc atacttctct gggccagtag cagatgacct cataaaatat actatattca
3301 atagttctgg tattcagatt ggaaaccaca attatatgga tgttggactg aattcacaac
3361 caccaaacaa tacttgcaaa gaagagtcga cttccagaca ccaagccatc tttgataaca
3421 ccactagtct gactgatgaa cacctgaacc ctatcaggga aaacctggga aggcagtgga
3481 aaaactgtgc ccgcaagctg ggcttcactg agtctcagat cgatgaaatc gaccatgact
3541 atgaaagaga tggactgaaa gagaaagttt accaaatgct tcagaagtgg ctgatgcggg
3601 aaggcaccaa aggggccaca gtgggaaagt tggcccaggc acttcaccaa tgttgcagga
3661 tagacctgct gaaccacttg attcgtgcca gccagagcta atctagaggg cccttcgaag
3721 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
3781 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
3841 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
3901 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
3961 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
4021 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
4081 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
4141 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
4201 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
4261 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
4321 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
4381 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
4441 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
4501 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
4561 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
4621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
4681 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
4741 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
4801 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
4861 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
4921 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
4981 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
5041 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
5101 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
5161 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
5221 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
5281 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
5341 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
5401 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
5461 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
5521 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
5581 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
5641 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
5701 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc
5761 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
5821 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
5881 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
5941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
6001 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
6061 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
6121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
6181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
6241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
6301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
6361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
6421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
6481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
6541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
6601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
6661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
6721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
6781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
6841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
6901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
6961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
7021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
7081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
7141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
7201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
7261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
7321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
7381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
7441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
7501 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
7561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
7621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
7681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
7741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
7801 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
7861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
7921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
7981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
8041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttc
//