LOCUS LT727174 8192 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1-hRIP1, complete sequence.
ACCESSION LT727174
VERSION LT727174.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1-hRIP1
ORGANISM Mammalian expression vector pEF1-hRIP1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8192)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8192
/organism="Mammalian expression vector pEF1-hRIP1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4717."
/db_xref="taxon:1944980"
regulatory 508..1651
/note="hEF1 promoter"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron"
regulatory 1668..1684
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1785..3800
/transl_table=11
/note="hRIP1"
/protein_id="SJL87668.1"
/translation="MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIM
KTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMH
VLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLA
SFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVL
WAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARP
TFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSN
SATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMD
RQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTVYSSAASHGNAVH
QPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPET
NYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKE
EPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGL
KEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN"
misc_feature 3823..3864
/note="V5 epitope"
misc_feature 3874..3891
/note="His-tag"
regulatory 3920..4144
/note="bovine growth hormone polyA"
/regulatory_class="terminator"
rep_origin 4190..4618
/note="f1 ori"
regulatory 4623..4904
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4861..4936
/note="SV40 ori"
CDS 5028..5822
/transl_table=11
/note="neomycin"
/protein_id="SJL87669.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5998..6072
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5998..6072)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(6386..6777)
/note="pMB1 ori"
CDS complement(7318..8178)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87670.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1959 a 2056 c 2195 g 1982 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
1741 tgtggtggaa ttctgcagat atccagcaca gtggcggccg caccatgcaa ccagacatgt
1801 ccttgaatgt cattaagatg aaatccagtg acttcctgga gagtgcagaa ctggacagcg
1861 gaggctttgg gaaggtgtct ctgtgtttcc acagaaccca gggactcatg atcatgaaaa
1921 cagtgtacaa ggggcccaac tgcattgagc acaacgaggc cctcttggag gaggcgaaga
1981 tgatgaacag actgagacac agccgggtgg tgaagctcct gggcgtcatc atagaggaag
2041 ggaagtactc cctggtgatg gagtacatgg agaagggcaa cctgatgcac gtgctgaaag
2101 ccgagatgag tactccgctt tctgtaaaag gaaggataat tttggaaatc attgaaggaa
2161 tgtgctactt acatggaaaa ggcgtgatac acaaggacct gaagcctgaa aatatccttg
2221 ttgataatga cttccacatt aagatcgcag acctcggcct tgcctccttt aagatgtgga
2281 gcaaactgaa taatgaagag cacaatgagc tgagggaagt ggacggcacc gctaagaaga
2341 atggcggcac cctctactac atggcgcccg agcacctgaa tgacgtcaac gcaaagccca
2401 cagagaagtc ggatgtgtac agctttgctg tagtactctg ggcgatattt gcaaataagg
2461 agccatatga aaatgctatc tgtgagcagc agttgataat gtgcataaaa tctgggaaca
2521 ggccagatgt ggatgacatc actgagtact gcccaagaga aattatcagt ctcatgaagc
2581 tctgctggga agcgaatccg gaagctcggc cgacatttcc tggcattgaa gaaaaattta
2641 ggccttttta tttaagtcaa ttagaagaaa gtgtagaaga ggacgtgaag agtttaaaga
2701 aagagtattc aaacgaaaat gcagttgtga agagaatgca gtctcttcaa cttgattgtg
2761 tggcagtacc ttcaagccgg tcaaattcag ccacagaaca gcctggttca ctgcacagtt
2821 cccagggact tgggatgggt cctgtggagg agtcctggtt tgctccttcc ctggagcacc
2881 cacaagaaga gaatgagccc agcctgcaga gtaaactcca agacgaagcc aactaccatc
2941 tttatggcag ccgcatggac aggcagacga aacagcagcc cagacagaat gtggcttaca
3001 acagagagga ggaaaggaga cgcagggtct cccatgaccc ttttgcacag caaagacctt
3061 acgagaattt tcagaataca gagggaaaag gcactgttta ttccagtgca gccagtcatg
3121 gtaatgcagt gcaccagccc tcagggctca ccagccaacc tcaagtactg tatcagaaca
3181 atggattata tagctcacat ggctttggaa caagaccact ggatccagga acagcaggtc
3241 ccagagtttg gtacaggcca attccaagtc atatgcctag tctgcataat atcccagtgc
3301 ctgagaccaa ctatctagga aatacaccca ccatgccatt cagctccttg ccaccaacag
3361 atgaatctat aaaatatacc atatacaata gtactggcat tcagattgga gcctacaatt
3421 atatggagat tggtgggacg agttcatcac tactagacag cacaaatacg aacttcaaag
3481 aagagccagc tgctaagtac caagctatct ttgataatac cactagtctg acggataaac
3541 acctggaccc aatcagggaa aatctgggaa agcactggaa aaactgtgcc cgtaaactgg
3601 gcttcacaca gtctcagatt gatgaaattg accatgacta tgagcgagat ggactgaaag
3661 aaaaggttta ccagatgctc caaaagtggg tgatgaggga aggcataaag ggagccacgg
3721 tggggaagct ggcccaggcg ctccaccagt gttccaggat cgaccttctg agcagcttga
3781 tttacgtcag ccagaactaa tctagagggc ccgcggttcg aaggtaagcc tatccctaac
3841 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
3901 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
3961 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
4021 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
4081 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
4141 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
4201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
4261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
4321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
4381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
4441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
4501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
4561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
4621 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
4681 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
4741 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
4801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
4861 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
4921 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
4981 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
5041 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
5101 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
5161 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
5221 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
5281 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
5341 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
5401 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
5461 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
5521 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
5581 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
5641 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
5701 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
5761 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
5821 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga
5881 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
5941 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
6001 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
6061 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
6121 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
6181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
6241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
6301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
6361 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
6421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
6481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagga accgtaaaaa
6541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
6601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
6661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
6721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
6781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
6841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
6901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
6961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
7021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
7081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
7141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
7201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
7261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
7321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
7381 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
7441 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
7501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
7561 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
7621 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
7681 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
7741 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
7801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
7861 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
7921 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
7981 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
8041 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
8101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
8161 gaaatgttga atactcatac tcttcctttt tc
//