LOCUS LT727173 5399 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pGal4-p65f520-550, complete sequence.
ACCESSION LT727173
VERSION LT727173.1
KEYWORDS .
SOURCE Mammalian expression vector pGal4-p65f520-550
ORGANISM Mammalian expression vector pGal4-p65f520-550
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5399)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5399
/organism="Mammalian expression vector pGal4-p65f520-550"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4714."
/db_xref="taxon:1945038"
misc_feature 1..825
/note="SV40 VP1 with internal deletion"
regulatory 826..900
/note="polyA"
/regulatory_class="terminator"
regulatory complement(826..900)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(933..1723)
/note="3UTR"
CDS complement(1724..>1819)
/codon_start=1
/transl_table=11
/note="p65f"
/protein_id="SJL87665.1"
/translation="PGLPNGLLSGDEDFSSIADMDFSALLSQISS"
misc_feature complement(1820..1828)
/note="link"
CDS complement(<1829..>2266)
/codon_start=1
/transl_table=11
/note="GALbd"
/protein_id="SJL87666.1"
/translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVN
KDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
regulatory complement(2410..2591)
/note="LTR"
/regulatory_class="promoter"
regulatory 2988..3269
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3226..3301
/note="SV40 ori"
rep_origin complement(3388..3790)
/note="pMB1 ori"
CDS complement(4331..5191)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87667.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1422 a 1316 c 1272 g 1389 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
901 aattccgcca cggatcaagc ttgatatcga attccttttt tttttttttt cagcttggca
961 acagctttat tagttcagag tagaaagagc aagagtccaa gtgctttgat tgttcactaa
1021 aaactatgcc tcctgactgg agagctgcca gcctgctctc ccccactctt aacaacttac
1081 cctactatta aggcacttga gaagagggga gagcaaggaa gtccagacca aaccccttct
1141 ggatcctgga gagagccagt gctgttgcac tgtttccttc agccatggtt gagcaaggaa
1201 agagccggca gagacctctg tagggcagga aggcagcccc tcaaacgctg gtgttaggca
1261 cagggacaat gccagtgcca tacaggggct ggtatctggg gcgttatttt gattaagctg
1321 taatgaatcc atgatggaag acacttgata aggctttgtg ggctcaaagg ccttacctcc
1381 agcctgcttc tgtctctagg agagtaccag aagctggagg atggggatgg gggaccccac
1441 agttccctac agagaaggga gctgaccatc aggacagggg aaaagtttga gtttccccag
1501 ctcccccctt tccagagaag ttaatgcttc tgcttaagca cctccaaaaa gagagagaga
1561 tacagatact gacaataaaa gaataaaata tggctccccc ctccaaggaa gacatccaca
1621 aagttggggg cagttggaac acaccccacc agaatccgta agtgcttttg gagggcttca
1681 atcccctgca accagtgctc tggggagggc aggcgtcacc cccttaggag ctgatctgac
1741 tcagcagggc tgagaagtcc atgtccgcaa tggaggagaa gtcttcatct cctgaaagga
1801 ggccattggg gagccccggg aattccggcg atacagtcaa ctgtctttga cctttgttac
1861 tactctcttc cgatgatgat gtcgcactta ttctatgctg tctcaatgtt agaggcatat
1921 cagtctccac tgaagccaat ctatctgtga cggcatcttt attcacatta tcttgtacaa
1981 ataatcctgt taacaatgct tttatatcct gtaaagaatc cattttcaaa atcatgtcaa
2041 ggtcttctcg aggaaaaatc agtagaaata gctgttccag tctttctagc cttgattcca
2101 cttctgtcag atgtgcccta gtcagcggag accttttggt tttgggagag tagcgacact
2161 cccagttgtt cttcagacac ttggcgcact tcggtttttc tttggagcac ttgagctttt
2221 taagtcggca aatatcgcat gcttgttcga tagaagacag tagcttcaga tctggctgct
2281 gggagtccat cagtgaatat caactctggc agaggagccg ctcgcccgcc accgtccgca
2341 gttcccgccg cagccgagat aaacaactta gcttgtgaac gcagaaggag caggaggtac
2401 ccagcttgga ggtgcacacc aatgtggtga atggtcaaat ggcgtttatt gtatcgagct
2461 aggcacttaa atacaatatc tctgcaatgc ggaattcagt ggttcgtcca atccatgtca
2521 gacctgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc
2581 atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc
2641 aagactacaa gtgtattgca taagactaca tttccccctc cctatgcaaa agcgaaacta
2701 ctatatcctg aggggactcc taaccgcgta caaccgaagc cccgcttttc gcctaaacac
2761 accctagtcc cctcagatac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
2821 cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta
2881 aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct
2941 taactatgcg gcatcagagc agattgtact gagagtgcac catatggctg tggaatgtgt
3001 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
3061 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
3121 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
3181 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
3241 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
3301 tttttggagg cctaggcttt tgcaaaaagc tccatatgcg gtgtgaaata ccgcacagat
3361 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
3421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
3481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
3541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
3601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
3661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
3721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
3781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
3841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
3901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
3961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
4021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
4081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
4141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
4201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
4261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
4321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
4381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
4441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
4501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
4561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
4621 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
4681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
4741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
4801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
4861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
4921 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
4981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
5041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
5101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
5161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
5221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
5281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
5341 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//