LOCUS LT727169 5209 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pGal4-p65f286-518, complete sequence.
ACCESSION LT727169
VERSION LT727169.1
KEYWORDS .
SOURCE Mammalian expression vector pGal4-p65f286-518
ORGANISM Mammalian expression vector pGal4-p65f286-518
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5209)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5209
/organism="Mammalian expression vector pGal4-p65f286-518"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4710."
/db_xref="taxon:1945034"
misc_feature 1..825
/note="SV40 VP1 with internal deletion"
regulatory 826..900
/note="SV40 polyA"
/regulatory_class="terminator"
regulatory complement(826..900)
/note="polyA"
/regulatory_class="terminator"
CDS complement(<928..>1635)
/codon_start=1
/transl_table=11
/note="p65f"
/protein_id="SJL87653.1"
/translation="EFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPP
RRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQV
LPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQ
FDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITR
LVTGAQRPPDPAPAPLGA"
CDS complement(<1639..>2076)
/codon_start=1
/transl_table=11
/note="GALbd"
/protein_id="SJL87654.1"
/translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVN
KDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
regulatory complement(2220..2401)
/note="LTR"
/regulatory_class="promoter"
regulatory 2798..3079
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3036..3111
/note="SV40 ori"
rep_origin complement(3198..3600)
/note="pMB1 ori"
CDS complement(4141..5001)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87655.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1310 a 1268 c 1302 g 1329 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
901 aattccgcca cggatcaagc ttgatggggc ccccagtgga gcaggagctg ggtcgggggg
961 cctctgggcc cctgtcacta ggcgagttat agcctcaggg tactccatca gcatgggctc
1021 agttgtgtgg ggggccacag gtatgccctg gttcagcagc tgctgaaact cggagttgtc
1081 gacggatgcc aggtctgtga acacagctgg gtctgtgctg ttgccaagca aggcccccag
1141 gtcttcatca tcaaactgca gctgcagcag ggcctctgac agcgttcctt ccccagcctg
1201 ggtgggcttg ggggcaggtg gggccacagc ctgaggaggg cctggggcta ggactgggac
1261 aggggctggg gcctgggcca gagctgatac catggctgga gcaggggcag gggctggagc
1321 ctggggcagg acttggggag gggccggggc caaggccgag gcctggctga tctgcccaga
1381 aggaaacacc atggtgggaa actcatcata gttgatggtg ctcagggatg acgtaaaggg
1441 atagggctgg ggtgctggct tggggacaga agctgagctg cgggaaggca cagcaatgcg
1501 tcgaggtgga ggccgggggt cggtgggtcc gctgaaagga ctcttcttca tgatgctctt
1561 gaaggtctca tatgtccttt tacgtttctc ctcaatccgg tgacgatcgt ctgtatctgg
1621 caggtactgg aattccggcg atacagtcaa ctgtctttga cctttgttac tactctcttc
1681 cgatgatgat gtcgcactta ttctatgctg tctcaatgtt agaggcatat cagtctccac
1741 tgaagccaat ctatctgtga cggcatcttt attcacatta tcttgtacaa ataatcctgt
1801 taacaatgct tttatatcct gtaaagaatc cattttcaaa atcatgtcaa ggtcttctcg
1861 aggaaaaatc agtagaaata gctgttccag tctttctagc cttgattcca cttctgtcag
1921 atgtgcccta gtcagcggag accttttggt tttgggagag tagcgacact cccagttgtt
1981 cttcagacac ttggcgcact tcggtttttc tttggagcac ttgagctttt taagtcggca
2041 aatatcgcat gcttgttcga tagaagacag tagcttcaga tctggctgct gggagtccat
2101 cagtgaatat caactctggc agaggagccg ctcgcccgcc accgtccgca gttcccgccg
2161 cagccgagat aaacaactta gcttgtgaac gcagaaggag caggaggtac ccagcttgga
2221 ggtgcacacc aatgtggtga atggtcaaat ggcgtttatt gtatcgagct aggcacttaa
2281 atacaatatc tctgcaatgc ggaattcagt ggttcgtcca atccatgtca gacctgtctg
2341 ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg
2401 ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa
2461 gtgtattgca taagactaca tttccccctc cctatgcaaa agcgaaacta ctatatcctg
2521 aggggactcc taaccgcgta caaccgaagc cccgcttttc gcctaaacac accctagtcc
2581 cctcagatac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta
2641 agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta aattttgctc
2701 gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct taactatgcg
2761 gcatcagagc agattgtact gagagtgcac catatggctg tggaatgtgt gtcagttagg
2821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
2881 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
2941 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
3001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
3061 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
3121 cctaggcttt tgcaaaaagc tccatatgcg gtgtgaaata ccgcacagat gcgtaaggag
3181 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
3241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
3301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
3361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
3421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
3481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
3541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
3601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
3661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
3721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
3781 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
3841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
3901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
3961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
4021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
4081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
4141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
4201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
4261 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
4321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
4381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
4441 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
4501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
4561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
4621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
4681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
4741 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
4801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
4861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
4921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
4981 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
5041 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
5101 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
5161 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//